FastQCFastQC Report
Thu 26 May 2016
SRR2132623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5169669
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3035505.871749235782794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370250.7161967236200228No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT217290.4203170454433349No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113640.2198206500261429No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97480.18856139532337565No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA88170.1705525053925116No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80280.15529040640706399No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG69170.1337996687989115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT56100.087.571151
AACGAGT756350.086.409621
ACGAGTG830000.085.252132
CGAGTGG868700.084.1142353
GAGTGGA921700.082.35324
GTGGAAT921350.082.3114856
TGGAATC927900.081.937317
GAATCAC930550.081.926179
GGAATCA930750.081.7217568
AGTGGAA931950.081.386945
ATCGAGT29200.075.830811
TCGAGTG33300.071.559342
GACGAGT14650.054.232141
ATGAGTG53250.051.457842
TGAGTGG59600.049.2043083
AATGAGT57200.044.3818471
GAGTACT496050.042.1164320-21
AGTACTT506850.041.7428820-21
TGAGTAC929800.041.44866618-19
ACGATTG8750.041.3604552
Produced by FastQC (version 0.11.5)