FastQCFastQC Report
Thu 26 May 2016
SRR2132622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5699790
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3166675.555766089627864No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA634201.112672572147395No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT231620.4063658485663507No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150640.2642904387705512No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG149890.26297460081862667No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121240.21270959105510906No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100450.1762345630277607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT58700.086.98121
AACGAGT830050.079.5861
ACGAGTG908050.078.9026262
CGAGTGG945200.078.296783
GAATCAC991950.077.7410669
GTGGAAT986100.077.7311556
ATCGAGT28200.077.524241
GGAATCA994500.077.324378
TGGAATC994800.077.2774357
GAGTGGA1001000.076.674064
AGTGGAA1006650.076.220375
TCGAGTG35050.069.063722
ATGAGTG60450.047.664492
GACGAGT17000.047.291261
TGAGTGG68850.044.0959173
GAGTACT500750.042.1982920-21
AATGAGT63350.042.1536671
AGTACTT513300.041.8165820-21
GTACTTT507850.040.94689622-23
CATGGGG266050.039.86680224-25
Produced by FastQC (version 0.11.5)