##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132622_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5699790
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.508761199974035	31.0	19.0	33.0	10.0	34.0
2	27.835273229364592	31.0	25.0	34.0	16.0	34.0
3	28.030408488733794	31.0	25.0	34.0	16.0	34.0
4	33.848883029023874	35.0	33.0	37.0	28.0	37.0
5	34.907051838751954	35.0	35.0	37.0	32.0	37.0
6	35.304992815524784	37.0	35.0	37.0	33.0	37.0
7	35.58424205102293	37.0	35.0	37.0	33.0	37.0
8	35.68636388358168	37.0	35.0	37.0	35.0	37.0
9	37.44870021527109	39.0	37.0	39.0	35.0	39.0
10-11	37.6458387063383	39.0	37.0	39.0	35.0	39.0
12-13	37.65575573836931	39.0	37.0	39.0	35.0	39.0
14-15	39.052682291803734	40.0	39.0	41.0	36.0	41.0
16-17	38.997675616119196	40.0	38.5	41.0	35.5	41.0
18-19	39.011969300623356	40.0	38.5	41.0	35.0	41.0
20-21	38.96705790915104	40.0	39.0	41.0	35.0	41.0
22-23	38.906207158509346	40.0	39.0	41.0	35.0	41.0
24-25	38.81125734456883	40.0	38.0	41.0	35.0	41.0
26-27	38.800560897857636	40.0	38.0	41.0	35.0	41.0
28-29	38.73045919235621	40.0	38.0	41.0	35.0	41.0
30-31	38.640346749617095	40.0	38.0	41.0	34.5	41.0
32-33	38.535161295416145	40.0	38.0	41.0	34.0	41.0
34-35	38.46055082731118	40.0	38.0	41.0	34.0	41.0
36-37	38.333589044508656	40.0	38.0	41.0	34.0	41.0
38-39	37.97141192921143	40.0	36.5	41.0	34.0	41.0
40-41	37.77687502522023	40.0	36.0	41.0	33.0	41.0
42-43	37.647703424161236	40.0	36.0	41.0	33.0	41.0
44-45	37.50553897599736	40.0	35.5	41.0	33.0	41.0
46-47	37.38198731883105	40.0	35.0	41.0	33.0	41.0
48-49	37.2306894639978	40.0	35.0	41.0	33.0	41.0
50-51	37.00414357371061	40.0	35.0	41.0	32.0	41.0
52-53	36.552945020781465	40.0	35.0	41.0	31.0	41.0
54-55	35.86932220309871	39.0	35.0	41.0	29.0	41.0
56-57	35.24600072985145	39.0	35.0	41.0	26.0	41.0
58-59	34.95926902570095	39.0	35.0	41.0	25.0	41.0
60-61	34.67289119423698	38.5	35.0	41.0	24.5	41.0
62-63	34.399165495570884	38.0	34.5	40.0	23.0	41.0
64-65	34.13357728968962	37.5	34.0	40.0	23.0	41.0
66-67	33.82890659129547	37.0	34.0	40.0	22.0	41.0
68-69	33.494231892753945	36.5	34.0	39.5	21.5	41.0
70-71	33.18231768187951	36.0	34.0	39.0	21.0	41.0
72-73	32.85785564029552	35.5	33.5	39.0	20.0	41.0
74-75	32.57097691318452	35.0	33.0	38.5	20.0	40.0
76-77	32.33062068953417	35.0	33.0	37.0	19.5	39.5
78-79	32.0258011260064	35.0	33.0	37.0	18.5	39.0
80-81	31.722676537205757	35.0	33.0	36.0	17.5	39.0
82-83	31.432614710366522	35.0	33.0	36.0	13.5	37.5
84-85	31.158125562520723	35.0	33.0	36.0	9.0	37.0
86-87	30.98631958370396	35.0	33.0	35.0	7.0	37.0
88-89	30.83923828772639	35.0	33.0	35.0	2.0	36.0
90-91	30.67325164611328	35.0	33.0	35.0	2.0	36.0
92-93	30.524471866507362	35.0	33.0	35.0	2.0	36.0
94-95	30.375880514896163	35.0	33.0	35.0	2.0	36.0
96-97	30.23670924718279	35.0	33.0	35.0	2.0	35.5
98-99	30.093435635347966	35.0	33.0	35.0	2.0	35.0
100	29.99330606917097	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	4.032645251069049
1101	2	4.141423733479073
1101	3	3.433890720382241
1101	4	1.9269788595690613
1101	5	1.0958659641395343
1101	6	0.8862137270202481
1101	7	0.5099823915996566
1101	8	0.5389677027611981
1101	9	0.6254342691458845
1101	10-11	0.46185058320102
1101	12-13	0.41304973868187744
1101	14-15	0.5529984347320465
1101	16-17	0.5219816172156797
1101	18-19	0.4546890288699288
1101	20-21	0.46478101566330565
1101	22-23	0.47503185340970333
1101	24-25	0.49755899014189
1101	26-27	0.43959230487531187
1101	28-29	0.49749255757198085
1101	30-31	0.5346650809303384
1101	32-33	0.4850235597176109
1101	34-35	0.4458564910692644
1101	36-37	0.4447792851694885
1101	38-39	0.4711985387866733
1101	40-41	0.40707315848978
1101	42-43	0.4580945302811372
1101	44-45	0.47746060307540006
1101	46-47	0.4251900971706135
1101	48-49	0.4157709855297398
1101	50-51	0.3777338425637069
1101	52-53	0.34978362604063307
1101	54-55	0.3737566137521
1101	56-57	0.3745092907532239
1101	58-59	0.376647110277851
1101	60-61	0.367350433377446
1101	62-63	0.3352592047044851
1101	64-65	0.35409213810226703
1101	66-67	0.297017756116027
1101	68-69	0.2896228628191224
1101	70-71	0.24587825500133675
1101	72-73	0.25009574508858634
1101	74-75	0.2763907709754534
1101	76-77	0.2488573686922564
1101	78-79	0.2818285524323052
1101	80-81	0.23386349125169303
1101	82-83	0.24172231717857784
1101	84-85	0.24711015959956484
1101	86-87	0.2588925993447653
1101	88-89	0.23051892372104987
1101	90-91	0.2375759544242655
1101	92-93	0.2372683457566218
1101	94-95	0.24277722822090197
1101	96-97	0.2990975908864222
1101	98-99	0.3178401168106113
1101	100	0.345111531140347
1102	1	2.958561575158818
1102	2	3.536121945759369
1102	3	4.286669128171841
1102	4	2.2290906267502635
1102	5	1.1904508392051198
1102	6	0.9355654570234435
1102	7	0.5378045484072942
1102	8	0.5471839843022721
1102	9	0.6673644018893157
1102	10-11	0.43472229139503327
1102	12-13	0.3856054324806877
1102	14-15	0.4968773232989179
1102	16-17	0.5406658147409331
1102	18-19	0.4573583663694336
1102	20-21	0.5068424655909496
1102	22-23	0.4155120513208175
1102	24-25	0.5094648226279261
1102	26-27	0.473200572587146
1102	28-29	0.4695278395788378
1102	30-31	0.478741440821004
1102	32-33	0.5269544633126841
1102	34-35	0.4836840818593018
1102	36-37	0.4766149418728389
1102	38-39	0.4736644468008677
1102	40-41	0.4311570789860184
1102	42-43	0.4869946042852078
1102	44-45	0.45492685942179634
1102	46-47	0.5117770967264832
1102	48-49	0.5448569773564884
1102	50-51	0.5396625015315522
1102	52-53	0.4496128540036253
1102	54-55	0.38923216163113494
1102	56-57	0.45718283884141897
1102	58-59	0.41185039173646487
1102	60-61	0.39515162070224363
1102	62-63	0.43709448421216024
1102	64-65	0.4561097616948828
1102	66-67	0.45009801201837263
1102	68-69	0.46398346920788214
1102	70-71	0.4962520195145359
1102	72-73	0.4411372331118315
1102	74-75	0.4769346224530011
1102	76-77	0.41920537934211666
1102	78-79	0.4448283242370401
1102	80-81	0.46594593755503766
1102	82-83	0.46689261801078885
1102	84-85	0.45049573534493703
1102	86-87	0.506031273421069
1102	88-89	0.5043569773209029
1102	90-91	0.5358582697825192
1102	92-93	0.5511213714976613
1102	94-95	0.5522351477315155
1102	96-97	0.5436350174257107
1102	98-99	0.5798756668188183
1102	100	0.6075609879055506
1103	1	2.6070959930597937
1103	2	3.2955645513237144
1103	3	3.015876617328974
1103	4	1.7326642659488698
1103	5	0.9070394583044674
1103	6	0.7746414352852682
1103	7	0.41700345627548074
1103	8	0.46383992861942147
1103	9	0.5603228286980126
1103	10-11	0.39494110755809686
1103	12-13	0.37190610529874846
1103	14-15	0.4770993160503565
1103	16-17	0.5228672998141803
1103	18-19	0.4314918885888588
1103	20-21	0.41564021189513767
1103	22-23	0.4248349779633358
1103	24-25	0.41786960362981773
1103	26-27	0.3841874055874044
1103	28-29	0.3259350724360175
1103	30-31	0.3190450400279232
1103	32-33	0.31346758070664293
1103	34-35	0.30457785327544684
1103	36-37	0.2952914030051801
1103	38-39	0.33841176971942133
1103	40-41	0.35608593815734935
1103	42-43	0.33570813940418276
1103	44-45	0.3245160192184855
1103	46-47	0.39424525293944157
1103	48-49	0.32981742239888234
1103	50-51	0.2977299018455781
1103	52-53	0.26066847482515954
1103	54-55	0.3135504776065332
1103	56-57	0.3632003600355844
1103	58-59	0.30626491588223104
1103	60-61	0.28775646567684987
1103	62-63	0.25868518561792087
1103	64-65	0.29413806356087946
1103	66-67	0.3367308719750355
1103	68-69	0.35878145230154956
1103	70-71	0.36700391498386153
1103	72-73	0.37070732405940277
1103	74-75	0.38676204387943613
1103	76-77	0.3482135384875562
1103	78-79	0.3138230499320649
1103	80-81	0.3374371296477854
1103	82-83	0.37535729529579953
1103	84-85	0.37446093542830283
1103	86-87	0.3766156854500373
1103	88-89	0.3809355520732467
1103	90-91	0.40350074639682987
1103	92-93	0.36442760041336086
1103	94-95	0.3914650857496298
1103	96-97	0.3639179842637965
1103	98-99	0.38263307554185033
1103	100	0.3734280366356124
1104	1	2.466432511470405
1104	2	3.333866641840231
1104	3	1.6812174292316726
1104	4	1.1315288141895863
1104	5	0.6116748947695854
1104	6	0.6270704386377801
1104	7	0.3566601322957652
1104	8	0.4057421115356874
1104	9	0.45087042667928046
1104	10-11	0.35328140403197494
1104	12-13	0.3454809172183033
1104	14-15	0.454251501506171
1104	16-17	0.4767322292999907
1104	18-19	0.3593190120976075
1104	20-21	0.4012336859527892
1104	22-23	0.3923229556610863
1104	24-25	0.41928319083655907
1104	26-27	0.3909803764857003
1104	28-29	0.458160103575203
1104	30-31	0.3955710268552366
1104	32-33	0.3085496554448568
1104	34-35	0.373020909380287
1104	36-37	0.31469219423181016
1104	38-39	0.29811065923542657
1104	40-41	0.32149826010585514
1104	42-43	0.3368652009296298
1104	44-45	0.2715946835790959
1104	46-47	0.2823227603465952
1104	48-49	0.23748042270828051
1104	50-51	0.3211329305139685
1104	52-53	0.38207706551997944
1104	54-55	0.39190248228062785
1104	56-57	0.38172202952806344
1104	58-59	0.3267372885122697
1104	60-61	0.38045845508008824
1104	62-63	0.41630528661129773
1104	64-65	0.40711891939201905
1104	66-67	0.37161610093372843
1104	68-69	0.38946099555410996
1104	70-71	0.3655895087514054
1104	72-73	0.30712281385152096
1104	74-75	0.3098048058835161
1104	76-77	0.3987132880761237
1104	78-79	0.40501622901304657
1104	80-81	0.4379746130026483
1104	82-83	0.3790899367708036
1104	84-85	0.4255498659578194
1104	86-87	0.43143970884404936
1104	88-89	0.4132921699324612
1104	90-91	0.38201465311801286
1104	92-93	0.32649182483860173
1104	94-95	0.35169185440638273
1104	96-97	0.37508051426473443
1104	98-99	0.3768453563388583
1104	100	0.361767954196349
1105	1	1.3512805111599029
1105	2	2.666180482871649
1105	3	1.3893047523045858
1105	4	0.9325680940473049
1105	5	0.5948742202599746
1105	6	0.547386090005304
1105	7	0.20643609280659803
1105	8	0.3084477045871026
1105	9	0.4630615710029886
1105	10-11	0.3120345211784681
1105	12-13	0.297523800421132
1105	14-15	0.38087368255919074
1105	16-17	0.4191393698699528
1105	18-19	0.33548200749691404
1105	20-21	0.33104382937965227
1105	22-23	0.2825612103085575
1105	24-25	0.3250694914715595
1105	26-27	0.30668661895707316
1105	28-29	0.316598595118883
1105	30-31	0.25248653503155083
1105	32-33	0.2005008678571656
1105	34-35	0.23094417940730239
1105	36-37	0.2236930716764931
1105	38-39	0.20903176129485956
1105	40-41	0.1632602605133826
1105	42-43	0.21436222759813006
1105	44-45	0.22778396073379525
1105	46-47	0.2655341974845413
1105	48-49	0.20730996209834274
1105	50-51	0.2205043723926181
1105	52-53	0.12778136388998718
1105	54-55	0.22310080418499467
1105	56-57	0.22986873952635278
1105	58-59	0.20445376298666673
1105	60-61	0.1910617086948676
1105	62-63	-0.09631473825655945
1105	64-65	0.01501022090724291
1105	66-67	0.18508491144864792
1105	68-69	0.24020105360684596
1105	70-71	0.24584996635335443
1105	72-73	0.1912010741545629
1105	74-75	0.16729877951581784
1105	76-77	0.19954237280290954
1105	78-79	0.21656432975423812
1105	80-81	0.190245590237609
1105	82-83	0.2017709461232826
1105	84-85	0.21867679186572886
1105	86-87	0.2607562030020425
1105	88-89	0.23299823636043016
1105	90-91	0.2744549924243458
1105	92-93	0.29309488369670333
1105	94-95	0.22494635633523785
1105	96-97	0.2569969061234936
1105	98-99	0.3065067985363221
1105	100	0.29156506763899515
1106	1	1.7590758370266109
1106	2	2.820091036948835
1106	3	1.2034462905484702
1106	4	0.8455733035586448
1106	5	0.5437925509080443
1106	6	0.5180683014368199
1106	7	0.26616888312958054
1106	8	0.3333012248932121
1106	9	0.35352226418353183
1106	10-11	0.22697729985753767
1106	12-13	0.1419072989193353
1106	14-15	0.1515936250190819
1106	16-17	0.25320641638325014
1106	18-19	0.003694471231845853
1106	20-21	0.18317135676518603
1106	22-23	0.19815549203915594
1106	24-25	0.2567618217529031
1106	26-27	0.17803827400309302
1106	28-29	0.19266589573197734
1106	30-31	0.14623834421283988
1106	32-33	0.0928801541624864
1106	34-35	0.10604668028921083
1106	36-37	0.1671818340429141
1106	38-39	0.16201675916805414
1106	40-41	0.11886576731218668
1106	42-43	0.1310945483484076
1106	44-45	0.08243736115692002
1106	46-47	0.052723088110866456
1106	48-49	-0.056574393172276416
1106	50-51	0.07044238321000762
1106	52-53	0.06565775503986515
1106	54-55	0.036409420960609395
1106	56-57	0.12727867836555617
1106	58-59	0.09460350967801645
1106	60-61	-0.08110414773781116
1106	62-63	0.05594200306704522
1106	64-65	-0.14556055193777695
1106	66-67	-0.2557068793592592
1106	68-69	-0.030471489466791013
1106	70-71	0.14844202866645162
1106	72-73	0.17075734888391736
1106	74-75	0.17053357238724232
1106	76-77	0.12620064161070843
1106	78-79	0.11170446160690162
1106	80-81	0.16537958599888825
1106	82-83	0.16969999084763998
1106	84-85	0.11907290367336287
1106	86-87	0.17589347739539463
1106	88-89	0.15413779253277937
1106	90-91	0.16199692354561535
1106	92-93	0.23745380990630593
1106	94-95	0.23660232381791246
1106	96-97	0.2514968553966064
1106	98-99	0.33342909146592703
1106	100	0.32091468849044347
1107	1	1.6431325288739878
1107	2	0.8893667793338089
1107	3	0.5915538442088533
1107	4	0.47369612310637166
1107	5	0.29916466161938615
1107	6	0.37685441861156477
1107	7	0.19518441345146442
1107	8	0.28209800065119595
1107	9	0.3289992731475877
1107	10-11	0.22831493616578058
1107	12-13	0.22884377355144636
1107	14-15	0.28458878929153997
1107	16-17	0.35037226532698895
1107	18-19	0.28069397943302477
1107	20-21	0.2992766501986779
1107	22-23	0.20943835615122453
1107	24-25	0.23326163457749516
1107	26-27	0.3331712932865756
1107	28-29	0.3110585891478408
1107	30-31	0.29400827957053366
1107	32-33	0.27765666225433705
1107	34-35	0.2534500260510839
1107	36-37	0.26736874466250526
1107	38-39	0.28695259525017747
1107	40-41	0.254624643257074
1107	42-43	0.2576367213036619
1107	44-45	0.2792008388748073
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2216	82-83	-1.017592520752416
2216	84-85	-1.0529405114399921
2216	86-87	-1.0975939025748147
2216	88-89	-1.1624223232322315
2216	90-91	-1.16333101846525
2216	92-93	-1.1903615932140958
2216	94-95	-1.231204113640679
2216	96-97	-1.2896816600085828
2216	98-99	-1.3669577467063618
2216	100	-1.3825942015855262
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3169.0
3	118.0
4	206.0
5	464.0
6	1031.0
7	1746.0
8	2512.0
9	3594.0
10	5405.0
11	8352.0
12	8625.0
13	11320.0
14	12356.0
15	13244.0
16	15735.0
17	20493.0
18	29654.0
19	50475.0
20	93039.0
21	111864.0
22	52469.0
23	33916.0
24	32909.0
25	35602.0
26	39060.0
27	46148.0
28	58234.0
29	72445.0
30	89439.0
31	112012.0
32	144187.0
33	189762.0
34	263523.0
35	432277.0
36	656051.0
37	1145687.0
38	1435625.0
39	464653.0
40	2389.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.765740008398907	38.576022122852514	26.825906607180556	16.83233126156802
2	19.87226187718892	37.083166642101645	24.93967532057356	18.10489616013588
3	21.003479270879573	24.47160876362188	21.593165680317643	32.9317462851809
4	31.86498099052772	25.71519301588304	23.083183766419467	19.336642227169772
5	18.242312399816967	36.92606176468111	23.990344531375012	20.84128130412691
6	34.09951636271638	23.193904408738245	24.415331544007554	18.29124768453782
7	19.693279228292614	23.192100322521387	37.61299784184539	19.5016226073406
8	33.30461297696933	23.419354046377148	24.210032299435593	19.066000677217932
9	33.148326517292745	24.961586304056816	23.81921088320798	18.070876295442464
10-11	18.14360704517184	39.35803775226807	24.06972011249537	18.42863509006472
12-13	18.03698030980089	24.593344667084228	31.34671628252971	26.022958740585178
14-15	18.229978648336168	31.793469583967127	24.11430947455959	25.862242293137115
16-17	25.69040262886878	24.36855568363045	31.403244680944386	18.53779700655638
18-19	18.415038097894833	24.459208146265038	31.399218216811498	25.72653553902863
20-21	25.80827539260218	31.802978706233038	24.030780081371418	18.35796581979336
22-23	25.550046580663498	24.605678454820264	31.422183975199086	18.422090989317148
24-25	18.391816241055714	31.68855601530459	24.0564988364357	25.863128907203993
26-27	18.604518757977566	27.470627195887413	35.44947649605453	18.475377550080488
28-29	25.0326966467877	24.379912885553534	32.16358771232425	18.423802755334517
30-31	23.636794755977768	25.116790271568195	32.42219347630693	18.82422149614711
32-33	20.418778399391105	26.3973491737705	33.28829726646485	19.89557516037355
34-35	20.205666478544956	26.05685112332172	33.72108396354691	20.016398434586414
36-37	20.17047986665497	26.146407579612248	33.78434950434249	19.898763049390297
38-39	20.685375899280732	26.063352560507436	33.34483437145335	19.90643716875848
40-41	20.16628726294871	26.601549244520463	33.27837623396863	19.9537872585622
42-43	20.350893446433528	26.220029865623996	33.38561265105095	20.04346403689153
44-45	20.30735021223793	26.407436118067174	33.14988566944784	20.13532800024705
46-47	20.54025120936377	26.183706081219206	33.2304335215336	20.04560918788343
48-49	20.260721388661064	26.44242461889626	33.15463036174718	20.1422236306955
50-51	20.5515478137924	26.629529797343253	32.69660139187774	20.12232099698661
52-53	20.679598686619688	26.624578207382914	32.380565859656826	20.315257246340572
54-55	21.012216858069284	27.280511901082566	31.119778793098046	20.5874924477501
56-57	21.486100871540042	27.75632390255679	29.93443711328196	20.823138112621205
58-59	21.777280719862137	28.370627444866827	29.161670085353265	20.690421749917775
60-61	21.546351149879044	28.577790672996162	28.95878354612637	20.91707463099842
62-63	21.19348205191958	28.73533520024825	28.832965275786098	21.238217472046063
64-65	21.364023982607872	28.636809477937863	29.100485512701063	20.89868102675321
66-67	21.32838506775294	28.266990522940265	29.41252125723518	20.99210315207161
68-69	21.48169228982269	28.21653920885033	29.410155661944888	20.891612839382095
70-71	20.885158206646572	28.563607115577295	29.60563282176133	20.945601856014807
72-73	20.951341362968893	28.195154936022206	29.695402015002998	21.15810168600591
74-75	21.06131124775699	28.42287176531531	29.31442690096186	21.201390085965844
76-77	21.29757325445392	28.29251269876039	29.350258054391038	21.05965599239465
78-79	21.24714568060221	28.578660787989534	28.86132503581332	21.312868495594937
80-81	21.41600742568851	28.7601386289934	28.68805555265578	21.135798392662316
82-83	21.45417551507938	28.756940711307116	28.662137542581156	21.12674623103235
84-85	21.493667403779416	29.001409894808795	28.28987444286698	21.21504825854481
86-87	21.50012219836347	29.118554167911604	28.204847266081366	21.176476367643556
88-89	21.907190268126204	28.919826362912882	28.062957304449494	21.11002606451142
90-91	21.669506709738826	29.258786040585207	27.887468501838903	21.18423874783707
92-93	21.514601970626316	29.38608922496143	27.777434196150136	21.321874608262117
94-95	21.6403965086188	29.434396245449364	27.68946883635247	21.23573840957937
96-97	21.57827637416385	29.196837418876378	27.83819847723872	21.386687729721057
98-99	21.939740270221783	29.336569245702858	27.372028891615212	21.351661592460143
100	21.648591391765464	29.644380699238127	27.380782574750757	21.326245334245648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69836.0
1	40273.5
2	7828.0
3	4144.5
4	3053.0
5	2655.0
6	2545.5
7	2606.5
8	2507.0
9	2403.5
10	2513.5
11	3989.0
12	26428.0
13	29781.5
14	11199.0
15	11195.0
16	13197.0
17	15619.5
18	18171.5
19	20863.0
20	23963.0
21	27633.0
22	32004.5
23	37393.0
24	43086.5
25	49288.5
26	56901.5
27	65337.0
28	73360.5
29	80475.0
30	88259.5
31	96848.5
32	106048.0
33	115930.5
34	126542.0
35	135077.0
36	141628.5
37	149006.5
38	154965.0
39	159746.0
40	164879.5
41	170789.0
42	176024.5
43	180244.5
44	185073.0
45	188392.0
46	189794.5
47	192231.0
48	192749.5
49	192560.0
50	201248.5
51	195060.5
52	173291.0
53	161560.5
54	153704.5
55	145765.5
56	133929.5
57	120235.5
58	109130.0
59	93448.5
60	75887.0
61	62985.5
62	51566.0
63	40797.5
64	31553.0
65	23653.0
66	17927.5
67	13991.0
68	10570.5
69	7801.0
70	6013.0
71	5014.5
72	4003.5
73	2774.5
74	1852.5
75	1285.0
76	890.0
77	590.0
78	401.5
79	249.0
80	150.5
81	107.0
82	70.0
83	48.0
84	31.0
85	18.5
86	14.0
87	12.0
88	7.5
89	6.5
90	6.0
91	2.0
92	4.0
93	5.5
94	2.5
95	1.0
96	0.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02507109911066899
2	0.011053038796166174
3	0.00561424192821139
4	0.0
5	0.003894880337696652
6	0.0011228483856422782
7	2.2807857833358775E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0010877593735909568
26-27	0.003772068795517028
28-29	8.158195301932175E-4
30-31	7.544137591034056E-4
32-33	0.004982639711287609
34-35	0.008166967554945007
36-37	0.005087906747441572
38-39	0.005342302084813651
40-41	0.008702074988727654
42-43	0.010447753338280883
44-45	0.009228410169497473
46-47	0.012614499832449966
48-49	0.016632191712326244
50-51	0.05937938064384828
52-53	0.010579337133473338
54-55	0.005780914735455165
56-57	0.003929969349747973
58-59	0.008359957121227274
60-61	0.015167225459183583
62-63	0.012746083627642422
64-65	0.011579373976935993
66-67	0.00971965633821597
68-69	0.006807268337956311
70-71	0.004675610855838549
72-73	0.0034299509280166464
74-75	0.0025000921086566347
76-77	0.002096568470066441
78-79	0.0016667280724377567
80-81	0.0020439349519894593
82-83	0.0015088275182068111
84-85	0.001298293445898884
86-87	7.193247470520844E-4
88-89	0.0015088275182068111
90-91	9.210865663471812E-4
92-93	5.000184217313269E-4
94-95	7.017802410264238E-4
96-97	0.0013772437230143567
98-99	0.003728207530452877
100	2.631675903849089E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5699790.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.77164102478623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.63332849723052	63.61359435735186
2	8.372313819259272	12.017894039654673
3	1.612079333573978	3.4710473769824453
4	0.4884117033977793	1.4021643779427917
5	0.2286598628378047	0.8205646796185888
6	0.13285534207842986	0.5721147551926948
7	0.08967545086127286	0.4505307987565794
8	0.06568121377052406	0.377123879744824
9	0.050892356167871376	0.3287365126007427
>10	0.27989676399900354	4.631465642685993
>50	0.03654884326146879	1.6203854368010429
>100	0.007535906320679096	1.1589649407126326
>500	0.001413941591037244	0.6887569290477662
>1k	4.8756251748513794E-4	0.6895402568217549
>5k	4.875625174307347E-5	0.18793077640381445
>10k+	1.7064688110075713E-4	7.969185239681758
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	316667	5.555766089627864	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63420	1.112672572147395	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23162	0.4063658485663507	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15064	0.2642904387705512	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	14989	0.26297460081862667	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12124	0.21270959105510906	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10045	0.1762345630277607	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010877593735909568	0.0	0.0	0.0	0.0
2	0.0010877593735909568	0.0	0.0	0.0	0.0
3	0.0011053038796166174	0.0	0.0	1.7544506025660593E-5	0.0
4	0.001122848385642278	0.0	0.0	3.5089012051321186E-5	0.0
5	0.001122848385642278	0.0	0.0	3.5089012051321186E-5	0.0
6	0.001122848385642278	0.0	0.0	5.263351807698178E-5	0.0
7	0.001122848385642278	0.0	0.0	5.263351807698178E-5	0.0
8	0.001122848385642278	0.0	0.0	5.263351807698178E-5	0.0
9	0.001122848385642278	0.0	0.0	5.263351807698178E-5	0.0
10-11	0.001122848385642278	0.0	0.0	7.017802410264237E-5	0.0
12-13	0.001122848385642278	0.0	0.0	7.017802410264237E-5	0.0
14-15	0.001122848385642278	0.0	0.0	9.649478314113327E-5	0.0
16-17	0.0011491651446807688	0.0	0.0	1.1403928916679386E-4	0.0
18-19	0.00117548190371926	0.0	0.0	1.2281154217962415E-4	0.0
20-21	0.00117548190371926	0.0	0.0	1.4912830121811507E-4	0.0
22-23	0.00117548190371926	0.0	0.0	1.5790055423094536E-4	0.0
24-25	0.0011842541567320902	0.0	0.0	1.6667280724377566E-4	0.0
26-27	0.0012105709157705811	0.0	0.0	1.7544506025660595E-4	0.0
28-29	0.0012281154217962417	0.0	0.0	2.1053407230792713E-4	0.0
30-31	0.001236887674809072	0.0	0.0	2.1053407230792713E-4	0.0
32-33	0.0012544321808347326	0.0	0.0	2.3685083134641801E-4	0.0
34-35	0.0013509269639758658	0.0	0.0	2.456230843592483E-4	1.7544506025660593E-5
36-37	0.0013947882290400173	0.0	0.0	2.631675903849089E-4	1.7544506025660593E-5
38-39	0.001561461036283793	0.0	0.0	2.8948434942339984E-4	1.7544506025660593E-5
40-41	0.0018772621447456835	0.0	0.0	3.158011084618907E-4	1.7544506025660593E-5
42-43	0.002272013530323047	1.7544506025660593E-5	0.0	3.508901205132119E-4	1.7544506025660593E-5
44-45	0.0027018539279517317	1.7544506025660593E-5	0.0	3.508901205132119E-4	1.7544506025660593E-5
46-47	0.00324573361474721	1.7544506025660593E-5	0.0	4.0352363859019367E-4	1.7544506025660593E-5
48-49	0.003982602867824954	1.7544506025660593E-5	0.0	4.2984039762868455E-4	1.7544506025660593E-5
50-51	0.005184401530582706	1.7544506025660593E-5	0.0	4.3861265064151485E-4	1.7544506025660593E-5
52-53	0.006359883434301965	1.7544506025660593E-5	0.0	4.3861265064151485E-4	1.7544506025660593E-5
54-55	0.008184512060970667	1.7544506025660593E-5	0.0	4.6492940968000573E-4	1.7544506025660593E-5
56-57	0.010570564880460508	1.7544506025660593E-5	0.0	4.7370166269283603E-4	1.7544506025660593E-5
58-59	0.01353558639879715	1.7544506025660593E-5	0.0	4.824739157056663E-4	1.7544506025660593E-5
60-61	0.018193652748610036	1.7544506025660593E-5	0.0	5.087906747441572E-4	1.7544506025660593E-5
62-63	0.024018428749129357	1.7544506025660593E-5	0.0	5.175629277569875E-4	1.7544506025660593E-5
64-65	0.03208890152093323	1.7544506025660593E-5	0.0	5.61424192821139E-4	1.7544506025660593E-5
66-67	0.04288754497972733	1.7544506025660593E-5	0.0	5.8774095185963E-4	1.7544506025660593E-5
68-69	0.05695823881230712	1.7544506025660593E-5	0.0	6.316022169237814E-4	1.7544506025660593E-5
70-71	0.0759413943320719	1.7544506025660593E-5	0.0	6.754634819879329E-4	1.7544506025660593E-5
72-73	0.1009949489367152	1.7544506025660593E-5	0.0	7.017802410264238E-4	1.7544506025660593E-5
74-75	0.13325052326489223	1.7544506025660593E-5	0.0	7.280970000649147E-4	1.7544506025660593E-5
76-77	0.17571700010000368	1.7544506025660593E-5	0.0	7.544137591034056E-4	1.7544506025660593E-5
78-79	0.23007865202051303	1.7544506025660593E-5	0.0	7.719582651290662E-4	1.7544506025660593E-5
80-81	0.3003005373882196	1.7544506025660593E-5	0.0	7.719582651290662E-4	1.7544506025660593E-5
82-83	0.3885230859382539	1.7544506025660593E-5	0.0	7.807305181418965E-4	1.7544506025660593E-5
84-85	0.4973428845624137	1.7544506025660593E-5	0.0	7.895027711547267E-4	1.7544506025660593E-5
86-87	0.6319267902852561	1.7544506025660593E-5	0.0	8.158195301932176E-4	1.7544506025660593E-5
88	0.7479749253919882	1.7544506025660593E-5	0.0	8.245917832060479E-4	1.7544506025660593E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	5870	0.0	86.9812	1
AACGAGT	83005	0.0	79.586	1
ACGAGTG	90805	0.0	78.902626	2
CGAGTGG	94520	0.0	78.29678	3
GAATCAC	99195	0.0	77.741066	9
GTGGAAT	98610	0.0	77.731155	6
ATCGAGT	2820	0.0	77.52424	1
GGAATCA	99450	0.0	77.32437	8
TGGAATC	99480	0.0	77.277435	7
GAGTGGA	100100	0.0	76.67406	4
AGTGGAA	100665	0.0	76.22037	5
TCGAGTG	3505	0.0	69.06372	2
ATGAGTG	6045	0.0	47.66449	2
GACGAGT	1700	0.0	47.29126	1
TGAGTGG	6885	0.0	44.095917	3
GAGTACT	50075	0.0	42.19829	20-21
AATGAGT	6335	0.0	42.153667	1
AGTACTT	51330	0.0	41.81658	20-21
GTACTTT	50785	0.0	40.946896	22-23
CATGGGG	26605	0.0	39.866802	24-25
>>END_MODULE