FastQCFastQC Report
Thu 26 May 2016
SRR2132621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5981725
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4235147.080131567399037No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1002581.6760717017248368No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT292300.4886550284407926No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG226550.37873690281649525No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT156530.2616803681212359No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154550.2583702861632723No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA136610.22837893751384425No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131870.22045480191750705No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71970.1203164638962841No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62100.10381620686340479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT75250.087.600261
AACGAGT1130550.079.646291
ACGAGTG1230750.079.234782
ATCGAGT36250.078.990191
CGAGTGG1282050.078.532953
GTGGAAT1335600.078.192496
TGGAATC1341450.078.000837
GAATCAC1348900.077.8138969
GGAATCA1346700.077.777018
AGTGGAA1349550.077.4042365
GAGTGGA1352300.077.257254
TCGAGTG42550.073.574272
ATGAGTG66850.056.0413062
GACGAGT23850.053.6221731
TGAGTGG74450.053.533793
AATGAGT69350.051.1875571
ACGATTG10950.044.215492
GAGTACT654600.042.35310420-21
AGTACTT662450.042.28395520-21
GTACTTT657400.041.5007522-23