##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132621_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5981725
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.637574278322724	31.0	23.0	33.0	16.0	34.0
2	27.919351524852782	31.0	25.0	34.0	16.0	34.0
3	28.081244289899654	31.0	25.0	34.0	16.0	34.0
4	33.869940527189065	35.0	33.0	37.0	28.0	37.0
5	34.91036883173332	35.0	35.0	37.0	32.0	37.0
6	35.298379982362945	37.0	35.0	37.0	33.0	37.0
7	35.54840301083718	37.0	35.0	37.0	33.0	37.0
8	35.67675444792263	37.0	35.0	37.0	35.0	37.0
9	37.45409676974452	39.0	37.0	39.0	35.0	39.0
10-11	37.649307515808566	39.0	37.0	39.0	35.0	39.0
12-13	37.66970363900046	39.0	37.0	39.0	35.0	39.0
14-15	39.07871525354308	40.0	39.0	41.0	36.0	41.0
16-17	39.01257898014369	40.0	39.0	41.0	35.5	41.0
18-19	39.00402392286506	40.0	38.5	41.0	35.0	41.0
20-21	38.94955451813649	40.0	39.0	41.0	35.0	41.0
22-23	38.876192068341496	40.0	39.0	41.0	35.0	41.0
24-25	38.797200389519745	40.0	38.0	41.0	35.0	41.0
26-27	38.78091938362262	40.0	38.5	41.0	35.0	41.0
28-29	38.6903844626759	40.0	38.0	41.0	35.0	41.0
30-31	38.577264501460704	40.0	38.0	41.0	34.5	41.0
32-33	38.45469642954164	40.0	38.0	41.0	34.0	41.0
34-35	38.379717472802575	40.0	38.0	41.0	34.0	41.0
36-37	38.23479071672469	40.0	38.0	41.0	34.0	41.0
38-39	37.800273332525315	40.0	36.0	41.0	33.5	41.0
40-41	37.58792564017905	40.0	35.0	41.0	33.0	41.0
42-43	37.44757323347362	40.0	35.0	41.0	33.0	41.0
44-45	37.29549118356327	40.0	35.0	41.0	33.0	41.0
46-47	37.16213466851117	40.0	35.0	41.0	33.0	41.0
48-49	36.99661510684627	40.0	35.0	41.0	32.5	41.0
50-51	36.74248448399082	40.0	35.0	41.0	31.5	41.0
52-53	36.21362775787921	39.0	35.0	41.0	30.5	41.0
54-55	35.38264856709394	39.0	35.0	41.0	27.0	41.0
56-57	34.64245932402443	39.0	35.0	41.0	22.0	41.0
58-59	34.31141727511713	39.0	35.0	41.0	19.5	41.0
60-61	34.00626976332078	38.0	34.0	40.5	17.5	41.0
62-63	33.71650936811706	37.5	34.0	40.0	14.5	41.0
64-65	33.44135228550293	37.0	34.0	40.0	12.5	41.0
66-67	33.1204112526069	36.5	34.0	40.0	9.0	41.0
68-69	32.78460059598193	36.0	33.5	39.5	7.5	41.0
70-71	32.473570082208724	35.5	33.0	39.0	6.0	41.0
72-73	32.143144159920425	35.0	33.0	39.0	2.0	40.5
74-75	31.847443672184863	35.0	33.0	37.5	2.0	40.0
76-77	31.603003147085495	35.0	33.0	37.0	2.0	39.5
78-79	31.294419251971632	35.0	33.0	37.0	2.0	39.0
80-81	30.99473730403855	35.0	33.0	36.0	2.0	39.0
82-83	30.6952099937727	35.0	33.0	36.0	2.0	37.0
84-85	30.413809478035184	35.0	33.0	35.5	2.0	37.0
86-87	30.241818622554533	35.0	32.5	35.0	2.0	37.0
88-89	30.08424417705595	35.0	33.0	35.0	2.0	36.0
90-91	29.906150148995483	35.0	32.0	35.0	2.0	36.0
92-93	29.747985990663228	35.0	32.0	35.0	2.0	36.0
94-95	29.588579381365744	35.0	32.0	35.0	2.0	36.0
96-97	29.432180683665663	35.0	32.0	35.0	2.0	35.0
98-99	29.277129506956605	35.0	32.0	35.0	2.0	35.0
100	29.172893939457264	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.9187588622803027
1101	2	4.0177971234214525
1101	3	3.3223108418221017
1101	4	1.870426324312838
1101	5	1.0709100316966627
1101	6	0.8797050967704934
1101	7	0.5008180597631195
1101	8	0.5464237582454246
1101	9	0.6371174620133644
1101	10-11	0.4602473305354309
1101	12-13	0.4162370855069568
1101	14-15	0.531690032842242
1101	16-17	0.5672325609786384
1101	18-19	0.48634901623475457
1101	20-21	0.5283416359575881
1101	22-23	0.4703008018220487
1101	24-25	0.533635868648922
1101	26-27	0.44470834550959637
1101	28-29	0.500973566789213
1101	30-31	0.5362373815315067
1101	32-33	0.4319434710076351
1101	34-35	0.44761832979769167
1101	36-37	0.3935193504378134
1101	38-39	0.43002960622702346
1101	40-41	0.4098441775296706
1101	42-43	0.46508960772334973
1101	44-45	0.4555083794751127
1101	46-47	0.49619573545400186
1101	48-49	0.4464283133572451
1101	50-51	0.4389955330855173
1101	52-53	0.4714929184079679
1101	54-55	0.4379316894294476
1101	56-57	0.4359724968011065
1101	58-59	0.3489397681991022
1101	60-61	0.3756795926779617
1101	62-63	0.33265307832174074
1101	64-65	0.36641985255421616
1101	66-67	0.3275411974734368
1101	68-69	0.36591945281519145
1101	70-71	0.3631240352525822
1101	72-73	0.3530406876244996
1101	74-75	0.3590788469407613
1101	76-77	0.3887464304569157
1101	78-79	0.3831794567338882
1101	80-81	0.372045892780811
1101	82-83	0.4159447830094294
1101	84-85	0.43830101163485224
1101	86-87	0.3995258989124295
1101	88-89	0.43022187395783007
1101	90-91	0.4308752785965986
1101	92-93	0.4381497997491728
1101	94-95	0.4493151492812473
1101	96-97	0.4217102093460525
1101	98-99	0.449616781841101
1101	100	0.43629586185194924
1102	1	2.943447941447367
1102	2	3.475247105537214
1102	3	4.2011709747851285
1102	4	2.178191273054736
1102	5	1.131714822180605
1102	6	0.9550198571173212
1102	7	0.5078661447807349
1102	8	0.5649154860671075
1102	9	0.6607109557490105
1102	10-11	0.48246653126826544
1102	12-13	0.4250072953522377
1102	14-15	0.5715383060811519
1102	16-17	0.6020633295568842
1102	18-19	0.5033947497865299
1102	20-21	0.5266946109815294
1102	22-23	0.45639462743686465
1102	24-25	0.5780490023776608
1102	26-27	0.46547517445483777
1102	28-29	0.4777966804075504
1102	30-31	0.46282095227517317
1102	32-33	0.48369154210701737
1102	34-35	0.482190149879294
1102	36-37	0.4757481083449022
1102	38-39	0.5118186523161086
1102	40-41	0.5314727255543588
1102	42-43	0.5851018185123849
1102	44-45	0.6011143785358968
1102	46-47	0.6041739190712363
1102	48-49	0.5668359066012272
1102	50-51	0.6027345231198211
1102	52-53	0.6875507289568432
1102	54-55	0.5971786373409813
1102	56-57	0.6806437409059782
1102	58-59	0.6447403814385027
1102	60-61	0.6821875380011093
1102	62-63	0.615940913178953
1102	64-65	0.6198105464283827
1102	66-67	0.6056074884777161
1102	68-69	0.5931846779477112
1102	70-71	0.5721051712999667
1102	72-73	0.5446341984299039
1102	74-75	0.5277965563983571
1102	76-77	0.5194819969167845
1102	78-79	0.55105525701779
1102	80-81	0.5466449561522033
1102	82-83	0.5252015200264388
1102	84-85	0.5271159425965379
1102	86-87	0.5297497863110259
1102	88-89	0.5488030856228328
1102	90-91	0.5925487626464587
1102	92-93	0.5726608094016008
1102	94-95	0.578971650385661
1102	96-97	0.625801385176139
1102	98-99	0.6209534227832592
1102	100	0.6370137090970296
1103	1	2.6188726072118413
1103	2	3.2092407320772764
1103	3	3.0415019694845
1103	4	1.7340717679575306
1103	5	0.9107392077410523
1103	6	0.741913118049844
1103	7	0.39772194568816843
1103	8	0.47505111342012185
1103	9	0.5389578656068963
1103	10-11	0.3782670551183358
1103	12-13	0.33551506867832614
1103	14-15	0.45685294292881196
1103	16-17	0.4924806555674124
1103	18-19	0.4332205445280337
1103	20-21	0.45122347918786687
1103	22-23	0.4171256168002415
1103	24-25	0.4516331190227376
1103	26-27	0.33235781326185787
1103	28-29	0.366015115195907
1103	30-31	0.35704823649605544
1103	32-33	0.3393865606292721
1103	34-35	0.36122171779124557
1103	36-37	0.35478434986282537
1103	38-39	0.3946026642354923
1103	40-41	0.3650108936134515
1103	42-43	0.373196150021478
1103	44-45	0.35550022886030064
1103	46-47	0.3734729575685449
1103	48-49	0.37841301943809214
1103	50-51	0.42554167642803975
1103	52-53	0.36263626402402593
1103	54-55	0.299842405802643
1103	56-57	0.28493546596002517
1103	58-59	0.28837874514742623
1103	60-61	0.23115939315356826
1103	62-63	0.2087475342730727
1103	64-65	0.25181700879068103
1103	66-67	0.27190627503881615
1103	68-69	0.2770655932673307
1103	70-71	0.2946235373020727
1103	72-73	0.2841101609550236
1103	74-75	0.33845703085637524
1103	76-77	0.28335527891076495
1103	78-79	0.2707380828059769
1103	80-81	0.23922558704884977
1103	82-83	0.2847874320747614
1103	84-85	0.32201444433770376
1103	86-87	0.3488493622291742
1103	88-89	0.3720586743093186
1103	90-91	0.3783581624243588
1103	92-93	0.3462538878303896
1103	94-95	0.3625111432100958
1103	96-97	0.34135875550123274
1103	98-99	0.38071103503727244
1103	100	0.4072455993470072
1104	1	2.405751414947538
1104	2	3.205102598174282
1104	3	1.5689191673235143
1104	4	1.07306196557073
1104	5	0.5972150356472099
1104	6	0.5857724147550272
1104	7	0.3643713645502942
1104	8	0.37530518951272995
1104	9	0.4499254836042752
1104	10-11	0.3360559165923789
1104	12-13	0.29326370836453464
1104	14-15	0.41243657908002973
1104	16-17	0.49240746207291153
1104	18-19	0.3518406462785322
1104	20-21	0.39345410519312196
1104	22-23	0.3541239007937733
1104	24-25	0.3786351111787951
1104	26-27	0.3904988693631708
1104	28-29	0.3556270765368055
1104	30-31	0.31776848588037865
1104	32-33	0.3004913067540471
1104	34-35	0.2505110851547059
1104	36-37	0.3017221487843642
1104	38-39	0.30701922480622557
1104	40-41	0.2918634509756757
1104	42-43	0.3023679944929185
1104	44-45	0.2282395080914128
1104	46-47	0.22840262023658653
1104	48-49	0.20593071402311125
1104	50-51	0.275767248618763
1104	52-53	0.23257747371623338
1104	54-55	0.20070730123293146
1104	56-57	0.236624400073417
1104	58-59	0.25173964033388074
1104	60-61	0.2865532803654034
1104	62-63	0.29587974850790033
1104	64-65	0.28173378755654
1104	66-67	0.2788029262863603
1104	68-69	0.34083012633208654
1104	70-71	0.32116506849150994
1104	72-73	0.24659385404476808
1104	74-75	0.3183682335247724
1104	76-77	0.2883463017402086
1104	78-79	0.3088690649887873
1104	80-81	0.3621533683900964
1104	82-83	0.3755680418481475
1104	84-85	0.3186258048916315
1104	86-87	0.32766850410587267
1104	88-89	0.36761641797323463
1104	90-91	0.39704997994582314
1104	92-93	0.3292291964552625
1104	94-95	0.3503189657244121
1104	96-97	0.3576040400622986
1104	98-99	0.36474411664963924
1104	100	0.40123521279761576
1105	1	1.331876717117737
1105	2	2.5555585882955114
1105	3	1.365805783909991
1105	4	0.8814776628358345
1105	5	0.5740124915745142
1105	6	0.5559737103003144
1105	7	0.2752053126844771
1105	8	0.3751828107960904
1105	9	0.48793961473192127
1105	10-11	0.3138472178986049
1105	12-13	0.30745952548416966
1105	14-15	0.36658884766379884
1105	16-17	0.39630009487189
1105	18-19	0.31873316176085353
1105	20-21	0.3503562579708799
1105	22-23	0.31880165679176997
1105	24-25	0.3425319703416889
1105	26-27	0.33232279906529527
1105	28-29	0.33791637713229505
1105	30-31	0.3588758205884446
1105	32-33	0.30970204953950287
1105	34-35	0.287527352038353
1105	36-37	0.27651046290524306
1105	38-39	0.287751269343687
1105	40-41	0.24230544632565199
1105	42-43	0.33490877343218983
1105	44-45	0.34524559499033103
1105	46-47	0.3216347374556463
1105	48-49	0.30742055005683966
1105	50-51	0.29203653788845685
1105	52-53	0.2321425698104278
1105	54-55	0.3957957236750431
1105	56-57	0.38256189184328804
1105	58-59	0.3461868571301636
1105	60-61	0.3270904948848923
1105	62-63	-0.01503093429671054
1105	64-65	0.03688486667026325
1105	66-67	0.18588617881485447
1105	68-69	0.2955927664607927
1105	70-71	0.3274681025465185
1105	72-73	0.28273386013064794
1105	74-75	0.29897087060712124
1105	76-77	0.28632154621546846
1105	78-79	0.2865092113644039
1105	80-81	0.28659380271027146
1105	82-83	0.323903348130699
1105	84-85	0.33283528045197386
1105	86-87	0.3599149368073249
1105	88-89	0.35001034587285673
1105	90-91	0.40922369228150046
1105	92-93	0.3805545326461477
1105	94-95	0.3280164251062274
1105	96-97	0.3617162454068108
1105	98-99	0.33813774778030137
1105	100	0.3612583591801872
1106	1	1.7729021484971383
1106	2	2.7923777040203284
1106	3	1.1760523423512268
1106	4	0.8576294466575618
1106	5	0.5984999167122069
1106	6	0.5821847209590558
1106	7	0.3133498443061242
1106	8	0.4033959672749745
1106	9	0.4128474774387243
1106	10-11	0.2833713539754328
1106	12-13	0.24338379817763212
1106	14-15	0.21437323778379636
1106	16-17	0.318003242585263
1106	18-19	0.11901874486272845
1106	20-21	0.24027591748973265
1106	22-23	0.22873197193129613
1106	24-25	0.30626388075908295
1106	26-27	0.2554477295729072
1106	28-29	0.28176753858920733
1106	30-31	0.2568255503962078
1106	32-33	0.2580680708633736
1106	34-35	0.27572963977065257
1106	36-37	0.31146962853100746
1106	38-39	0.3062929033845876
1106	40-41	0.2607382837496246
1106	42-43	0.26564986659462875
1106	44-45	0.15004811408014973
1106	46-47	0.1813081610353109
1106	48-49	0.09687156098878802
1106	50-51	0.15181830235117388
1106	52-53	0.13718724900321178
1106	54-55	0.11657010937869217
1106	56-57	0.1076868369118884
1106	58-59	0.1816448780593305
1106	60-61	0.01898133957728021
1106	62-63	0.11519536412933462
1106	64-65	-0.13161801930108652
1106	66-67	-0.22758564427920192
1106	68-69	0.01849114209360181
1106	70-71	0.0925951730228789
1106	72-73	0.12060884035544461
1106	74-75	0.12293788816749185
1106	76-77	0.10702109253970704
1106	78-79	0.07451509580021565
1106	80-81	0.06275029690732126
1106	82-83	0.0784798924217931
1106	84-85	0.05597834579206662
1106	86-87	0.1094939202873988
1106	88-89	0.052165020757765745
1106	90-91	0.0894395240195145
1106	92-93	0.13362867425760783
1106	94-95	0.1417616693966366
1106	96-97	0.12384344482772747
1106	98-99	0.14215056445725338
1106	100	0.130528759513588
1107	1	1.6688341229622026
1107	2	0.8956394137214403
1107	3	0.4896437530051898
1107	4	0.40220487699493646
1107	5	0.2710734249316147
1107	6	0.3829813561682087
1107	7	0.1714538459566839
1107	8	0.2325872979310546
1107	9	0.3110174722578378
1107	10-11	0.20491292963207997
1107	12-13	0.19558117854638368
1107	14-15	0.25530745985947334
1107	16-17	0.2746545349062899
1107	18-19	0.21514043472025435
1107	20-21	0.22392236029224222
1107	22-23	0.17794460786881672
1107	24-25	0.17587280780868042
1107	26-27	0.19019211843942685
1107	28-29	0.23379279699948086
1107	30-31	0.17459417318727333
1107	32-33	0.10163078586403174
1107	34-35	0.12643830498590347
1107	36-37	0.18914982440720962
1107	38-39	0.1942569443258364
1107	40-41	0.1990165185707511
1107	42-43	0.11337474753396037
1107	44-45	0.10141066200248616
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2216	72-73	-1.1979746115986813
2216	74-75	-1.1200668920139165
2216	76-77	-1.0573709836991867
2216	78-79	-1.10444175997403
2216	80-81	-1.0521549979456637
2216	82-83	-1.0947694708524374
2216	84-85	-1.0864593740716586
2216	86-87	-1.0698635581235827
2216	88-89	-1.1002245277434533
2216	90-91	-1.0810528210036416
2216	92-93	-1.1091528862046651
2216	94-95	-1.1190429463684666
2216	96-97	-1.1636387366626977
2216	98-99	-1.179377235366207
2216	100	-1.183696526859439
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6787.0
3	138.0
4	394.0
5	732.0
6	1327.0
7	2123.0
8	3016.0
9	4300.0
10	6429.0
11	10685.0
12	10523.0
13	13914.0
14	14928.0
15	16282.0
16	19704.0
17	25218.0
18	36813.0
19	62961.0
20	119313.0
21	144715.0
22	66040.0
23	41446.0
24	40577.0
25	42256.0
26	46543.0
27	53453.0
28	65541.0
29	79290.0
30	95443.0
31	117270.0
32	148116.0
33	194099.0
34	268962.0
35	455889.0
36	677177.0
37	1169881.0
38	1448308.0
39	468968.0
40	2164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.36293670335734	42.05390310484225	25.111907260239043	15.471252931561366
2	18.656225808770788	41.33802263504871	23.275360768033384	16.730390788147112
3	19.68530976327513	23.494735490541135	21.18245740861548	35.637497337568256
4	35.539881890257405	24.865803760620892	21.669167338852922	17.925147010268777
5	17.255975467019223	41.48252804188382	22.636716983485243	18.62477950761172
6	37.053994448356406	23.06326078481329	22.90325481990594	16.97948994692436
7	18.2107502966541	22.868829903937232	40.929004629776685	17.99141516963198
8	36.45642352331476	23.378975128411955	22.509794415490514	17.65480693278277
9	36.16476852412975	24.252067756374622	22.46000944543589	17.12315427405974
10-11	17.064843335325513	43.044648491864805	22.654685730286833	17.23582244252285
12-13	16.892727432304227	23.980641035821606	32.0701135542005	27.056517977673668
14-15	17.095946403420417	33.25521484187254	22.786712862928336	26.86212589177871
16-17	26.722968708859067	23.743410136708057	32.127137238839964	17.40648391559291
18-19	17.35513585128036	23.855969640864465	32.147658075220775	26.641236432634397
20-21	26.829601828903872	33.33555955848856	22.70523135048836	17.129607262119205
22-23	26.5766146053187	23.939733103745155	32.248740956831014	17.234911334105128
24-25	17.259962266317945	33.17430274563484	22.749217462013252	26.816517526033955
26-27	17.499201076254565	28.013279980914437	37.24696603326482	17.24055290956618
28-29	26.218120294020807	23.767682202616637	32.76120083545015	17.252996667912413
30-31	24.167648761816174	24.73809008148123	33.263163578754444	17.831097577948153
32-33	19.893031928419568	26.560463743421074	34.35733642112204	19.18916790703732
34-35	19.7504344867539	26.09837164646864	34.72985749503235	19.42133637174511
36-37	19.58485868463434	26.21618083632134	34.99336861933367	19.205591859710648
38-39	20.11291881451378	26.12284763190817	34.514962022958926	19.24927153061912
40-41	19.559911275373867	26.941503239398497	34.38138615258502	19.117199332642613
42-43	19.735513025764455	26.19916090524325	34.56011393243889	19.505212136553403
44-45	19.682681768985645	26.700605476549562	34.13659214568286	19.48012060878193
46-47	20.076537797026617	26.320261114988693	34.253276623435355	19.349924464549336
48-49	19.721192944474513	26.619438326184543	34.17922491414511	19.480143815195834
50-51	20.019794200866226	26.94830816290395	33.66599121932394	19.365906416905883
52-53	20.158368425025664	26.896316097504535	33.24905977002789	19.696255707441914
54-55	20.655512086320698	27.690506329695904	31.599251642955682	20.05472994102772
56-57	21.068342821107755	28.32472856377517	30.15249011969383	20.45443849542324
58-59	21.612115948159296	28.965288363595477	29.17036401094595	20.252231677299275
60-61	21.38877684056613	29.098712087550123	29.02645589784983	20.48605517403392
62-63	20.729837867960462	29.34408422997752	29.079736968581205	20.84634093348081
64-65	21.05964981798711	29.27691832472069	29.317471523520393	20.345960333771803
66-67	20.920731194017335	28.716566726273392	29.711099799645503	20.65160228006377
68-69	21.153940839240796	28.782323511938323	29.644136148942383	20.419599499878498
70-71	20.28621714803504	29.279554490971915	29.806498316259084	20.62773004473397
72-73	20.373733341971022	28.73691755673186	29.972477811933807	20.91687128936331
74-75	20.59063112792271	28.90267620561074	29.54902668348161	20.957665982984945
76-77	20.953000232044783	28.67025299568477	29.75046492546147	20.626281846808983
78-79	20.869268294313933	29.136875505714148	29.126343277094573	20.86751292287734
80-81	20.968827385592096	29.234784098769357	28.998326120256035	20.798062395382516
82-83	21.04675658293655	29.352933201084184	28.846742506527058	20.75356770945221
84-85	20.94682571837703	29.61346602669944	28.50033030125999	20.939377953663534
86-87	21.026530836398667	29.946105450959354	28.330004558919143	20.697359153722843
88-89	21.63427250814744	29.531370525177568	28.03082736255679	20.803529604118207
90-91	21.47229920301268	29.97271154736376	27.83157191885652	20.72341733076704
92-93	21.117749612003365	30.104367688153605	27.843158332300323	20.93472436754271
94-95	21.25779025366315	30.273570621463257	27.636189741565616	20.83244938330798
96-97	21.105004259712747	29.75207903405497	27.94449300056306	21.19842370566923
98-99	21.55951546229395	29.970906739398146	27.254786210844383	21.214791587463516
100	21.20786784523633	30.355379745671556	27.31315806807213	21.123594341019984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	101434.0
1	57510.5
2	9719.0
3	4910.5
4	3668.5
5	3161.0
6	2923.0
7	2985.5
8	2992.5
9	2950.5
10	3172.0
11	5485.0
12	40111.5
13	44629.0
14	15247.5
15	14834.0
16	17250.0
17	20018.5
18	22836.5
19	26007.5
20	29676.5
21	33680.5
22	38658.0
23	45314.5
24	51738.5
25	58297.5
26	67998.5
27	77903.0
28	84619.5
29	90324.5
30	96568.5
31	102556.5
32	109995.5
33	120005.5
34	130997.5
35	139641.0
36	146623.5
37	153911.5
38	158943.5
39	161517.0
40	165429.0
41	170316.0
42	173636.5
43	175554.0
44	177957.5
45	181190.5
46	184131.5
47	189429.5
48	190790.5
49	194201.5
50	214656.5
51	210621.5
52	180751.5
53	167372.5
54	161358.5
55	155234.5
56	142409.5
57	126716.5
58	120482.0
59	104243.0
60	79122.0
61	62436.5
62	50170.0
63	38740.5
64	28567.5
65	21545.5
66	16245.5
67	12396.5
68	9755.0
69	7253.5
70	5166.5
71	3946.0
72	3008.5
73	2078.0
74	1448.5
75	1044.0
76	691.5
77	444.0
78	307.5
79	215.5
80	141.5
81	101.0
82	62.5
83	46.0
84	41.0
85	32.0
86	27.5
87	20.0
88	16.5
89	17.5
90	13.5
91	10.5
92	10.0
93	7.0
94	3.0
95	4.0
96	3.5
97	1.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025912257751735494
2	0.012287425450016508
3	0.005867872561844618
4	0.0
5	0.0037781743560594983
6	0.0012203837521785105
7	1.838934421090906E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001111719445477684
26-27	0.004020579347930571
28-29	6.60344633028098E-4
30-31	6.77062218674379E-4
32-33	0.0052493218929322225
34-35	0.00886032039252891
36-37	0.005282757064224784
38-39	0.0058177198049057756
40-41	0.008726579707358663
42-43	0.010640743263857834
44-45	0.009503947439910728
46-47	0.012680288712704112
48-49	0.016617280132403277
50-51	0.0620473191261718
52-53	0.010172650865761967
54-55	0.005943101697252882
56-57	0.004271343132624786
58-59	0.008651350571950399
60-61	0.01542197275869419
62-63	0.01281402939787436
64-65	0.011576928060049568
66-67	0.009637688125080975
68-69	0.0063276061671173455
70-71	0.004221190375685944
72-73	0.003134547308677681
74-75	0.0023488207833024756
76-77	0.0020228278631999968
78-79	0.001420994779933882
80-81	0.0018807283852066083
82-83	0.0014377123655801628
84-85	0.0010782842741851222
86-87	7.355737684363624E-4
88-89	0.0015213002938115677
90-91	0.0012371013378247914
92-93	6.101918760892552E-4
94-95	8.693144536066102E-4
96-97	0.0016884761502743773
98-99	0.004530465710142141
100	1.838934421090906E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5981725.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.79905812023184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.14633520257041	54.96055046433764
2	9.652305136318184	12.316159527723523
3	2.1574044203113862	4.129211100008737
4	0.7132458297981782	1.8201764859722784
5	0.34581870687234817	1.103145388940618
6	0.20652541279522893	0.7905676088536608
7	0.14759239859101356	0.6591379211068749
8	0.10008125596470578	0.5108071892830451
9	0.0710063030979595	0.4077121732424564
>10	0.33879999770814606	4.087014562906488
>50	0.10000319215835132	4.453471317243587
>100	0.017403961581082875	1.8453331497450671
>500	0.0022490549321101953	1.0144892055223298
>1k	9.153075645605107E-4	0.9263569756593102
>5k	1.0460657878943182E-4	0.40434893020170243
>10k+	2.0921315757886364E-4	10.571517999252785
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	423514	7.080131567399037	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	100258	1.6760717017248368	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29230	0.4886550284407926	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	22655	0.37873690281649525	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15653	0.2616803681212359	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15455	0.2583702861632723	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	13661	0.22837893751384425	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13187	0.22045480191750705	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7197	0.1203164638962841	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6210	0.10381620686340479	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011033606526545435	1.6717585646280965E-5	0.0	0.0	1.6717585646280965E-5
2	0.0011033606526545435	1.6717585646280965E-5	0.0	1.6717585646280965E-5	1.6717585646280965E-5
3	0.0011535134095933864	1.6717585646280965E-5	0.0	1.6717585646280965E-5	1.6717585646280965E-5
4	0.0011535134095933864	5.0152756938842894E-5	0.0	1.6717585646280965E-5	3.343517129256193E-5
5	0.0011535134095933864	6.687034258512386E-5	0.0	1.6717585646280965E-5	6.687034258512386E-5
6	0.0011535134095933864	1.1702309952396675E-4	0.0	1.6717585646280965E-5	6.687034258512386E-5
7	0.0011535134095933864	1.3374068517024772E-4	0.0	3.343517129256193E-5	8.358792823140482E-5
8	0.0011535134095933864	1.5045827081652867E-4	0.0	5.0152756938842894E-5	8.358792823140482E-5
9	0.0011535134095933864	1.6717585646280965E-4	0.0	6.687034258512386E-5	8.358792823140482E-5
10-11	0.001178589788062808	1.838934421090906E-4	0.0	9.194672105454531E-5	8.358792823140482E-5
12-13	0.0011869485808859485	2.0061102775537158E-4	0.0	1.0866430670082626E-4	8.358792823140482E-5
14-15	0.0011869485808859485	2.1732861340165253E-4	0.0	1.420994779933882E-4	1.0030551387768579E-4
16-17	0.0012120249593553698	2.507637846942145E-4	0.0	1.838934421090906E-4	1.3374068517024772E-4
18-19	0.0012371013378247914	2.841989559867764E-4	0.0	1.838934421090906E-4	1.3374068517024772E-4
20-21	0.0012788953019404938	3.0091654163305734E-4	0.0	1.838934421090906E-4	1.838934421090906E-4
22-23	0.0012872540947636343	3.0927533445619784E-4	0.0	2.0061102775537158E-4	2.0896982057851205E-4
24-25	0.0013123304732330556	3.1763412727933834E-4	0.0	2.0896982057851205E-4	2.2568740622479303E-4
26-27	0.001337406851702477	3.5106929857190025E-4	0.0	2.1732861340165253E-4	2.507637846942145E-4
28-29	0.001354124437348758	3.8450446986446215E-4	0.0	2.340461990479335E-4	2.6748137034049544E-4
30-31	0.0014209947799338819	3.9286326268760265E-4	0.0	2.507637846942145E-4	2.841989559867764E-4
32-33	0.001546376672280989	4.262984339801646E-4	0.0	2.507637846942145E-4	3.0927533445619784E-4
34-35	0.001663399771804956	4.68092398095867E-4	0.0	2.6748137034049544E-4	3.427105057487598E-4
36-37	0.0017887816641520631	4.931687765652884E-4	0.0	2.758401631636359E-4	3.5106929857190025E-4
38-39	0.0020228278631999963	5.516803263272718E-4	0.0	2.841989559867764E-4	3.5106929857190025E-4
40-41	0.0024909202612958636	5.851154976198338E-4	0.0	2.925577488099169E-4	3.5106929857190025E-4
42-43	0.003184700065616524	6.018330832661147E-4	0.0	3.343517129256193E-4	3.677868842181812E-4
44-45	0.004179396411570242	6.269094617355362E-4	0.0	3.677868842181812E-4	3.677868842181812E-4
46-47	0.005491726884803297	6.436270473818171E-4	0.0	3.761456770413217E-4	4.430160196264455E-4
48-49	0.007038103557084286	6.687034258512386E-4	0.0	4.0122205551074315E-4	4.51374812449586E-4
50-51	0.008902114356644612	6.854210114975196E-4	0.0	4.0122205551074315E-4	4.51374812449586E-4
52-53	0.011627080816988409	7.43932561259503E-4	0.0	4.095808483338836E-4	4.68092398095867E-4
54-55	0.014769986918489231	8.024441110214863E-4	0.0	4.3465722680330506E-4	4.68092398095867E-4
56-57	0.019551216413325585	8.525968679603292E-4	0.0	4.51374812449586E-4	5.098863622115694E-4
58-59	0.025218477947414834	8.943908320760316E-4	0.0	4.597336052727265E-4	5.767567047966933E-4
60-61	0.033309789400214826	9.19467210545453E-4	0.0	4.764511909190075E-4	6.018330832661147E-4
62-63	0.043465722680330506	9.36184796191734E-4	0.0	4.8480998374214796E-4	6.018330832661147E-4
64-65	0.057943151850009825	9.863375531305768E-4	0.0	4.931687765652884E-4	6.018330832661147E-4
66-67	0.07644116036761972	9.863375531305768E-4	0.0	5.01527569388429E-4	6.352682545586767E-4
68-69	0.10077360627578165	9.946963459537173E-4	0.0	5.01527569388429E-4	7.021385971438005E-4
70-71	0.1323531255616064	0.0010114139315999984	0.0	5.01527569388429E-4	7.355737684363624E-4
72-73	0.17136361166720301	0.0010364903100694197	0.0	5.01527569388429E-4	7.606501469057838E-4
74-75	0.2251273671056426	0.0010364903100694197	0.0	5.098863622115694E-4	7.773677325520647E-4
76-77	0.29178873987018794	0.0010699254813619817	0.0	5.182451550347099E-4	7.857265253752053E-4
78-79	0.37733262562220765	0.0010699254813619817	0.0	5.182451550347099E-4	8.358792823140482E-4
80-81	0.484157997901943	0.001095001859831403	0.0	5.349627406809909E-4	8.693144536066101E-4
82-83	0.6154328391893642	0.0011367958239471055	0.0	5.516803263272718E-4	8.693144536066101E-4
84-85	0.7777438782291062	0.0011869485808859485	0.0	5.516803263272718E-4	8.943908320760316E-4
86-87	0.9739999749236216	0.0012120249593553698	0.0	5.516803263272718E-4	9.02749624899172E-4
88	1.145689579510927	0.0012538189234710723	0.0	5.516803263272718E-4	9.02749624899172E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	7525	0.0	87.60026	1
AACGAGT	113055	0.0	79.64629	1
ACGAGTG	123075	0.0	79.23478	2
ATCGAGT	3625	0.0	78.99019	1
CGAGTGG	128205	0.0	78.53295	3
GTGGAAT	133560	0.0	78.19249	6
TGGAATC	134145	0.0	78.00083	7
GAATCAC	134890	0.0	77.813896	9
GGAATCA	134670	0.0	77.77701	8
AGTGGAA	134955	0.0	77.404236	5
GAGTGGA	135230	0.0	77.25725	4
TCGAGTG	4255	0.0	73.57427	2
ATGAGTG	6685	0.0	56.041306	2
GACGAGT	2385	0.0	53.622173	1
TGAGTGG	7445	0.0	53.53379	3
AATGAGT	6935	0.0	51.187557	1
ACGATTG	1095	0.0	44.21549	2
GAGTACT	65460	0.0	42.353104	20-21
AGTACTT	66245	0.0	42.283955	20-21
GTACTTT	65740	0.0	41.50075	22-23
>>END_MODULE