FastQCFastQC Report
Thu 26 May 2016
SRR2132620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4848758
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3849687.939517707421158No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920371.8981561876257795No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT270070.5569879956887929No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG215220.4438662436854964No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175410.3617627441913991No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140180.28910496254917234No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122570.25278638364711126No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA87180.17979862059521223No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC71690.1478522953713095No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62400.1286927497722097No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60070.12388739549385637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT68250.089.900741
AACGAGT1040300.079.729681
ACGAGTG1131000.079.380562
CGAGTGG1172800.078.785223
GTGGAAT1217550.078.402096
ATCGAGT29400.078.041911
GAATCAC1228800.078.035349
TGGAATC1235350.077.424577
AGTGGAA1232800.077.4109655
GAGTGGA1236650.077.2345664
GGAATCA1238650.077.190958
TCGAGTG34650.075.1536252
GACGAGT16550.065.625011
ATGAGTG55450.055.8633842
TGAGTGG59800.055.7224543
AATGAGT55000.051.376821
ACGATTG8400.045.885762
AGTACTT623700.042.73380320-21
GAGTACT617650.042.56652520-21
GTACTTT623850.041.54084422-23