##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132620_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4848758 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.729719033203967 31.0 23.0 33.0 16.0 34.0 2 28.00392079786205 31.0 25.0 34.0 16.0 34.0 3 28.177988466324777 31.0 26.0 34.0 16.0 34.0 4 33.943835720405104 35.0 33.0 37.0 28.0 37.0 5 34.96372452491958 37.0 35.0 37.0 32.0 37.0 6 35.34753497699823 37.0 35.0 37.0 33.0 37.0 7 35.58026797790279 37.0 35.0 37.0 33.0 37.0 8 35.71560717198095 37.0 35.0 37.0 35.0 37.0 9 37.49811395000534 39.0 37.0 39.0 35.0 39.0 10-11 37.694635512846794 39.0 37.0 39.0 35.0 39.0 12-13 37.71783310282757 39.0 38.0 39.0 35.0 39.0 14-15 39.13151862806929 41.0 39.0 41.0 36.0 41.0 16-17 39.056439504714405 40.5 39.0 41.0 36.0 41.0 18-19 39.033347302546346 40.0 38.5 41.0 35.0 41.0 20-21 38.970207525308545 40.0 39.0 41.0 35.0 41.0 22-23 38.88577868806816 40.0 39.0 41.0 35.0 41.0 24-25 38.81432317306824 40.0 38.5 41.0 35.0 41.0 26-27 38.80166632362349 40.0 38.5 41.0 35.0 41.0 28-29 38.707571815297854 40.0 38.0 41.0 35.0 41.0 30-31 38.58120378455679 40.0 38.0 41.0 34.5 41.0 32-33 38.45606349089808 40.0 38.0 41.0 34.0 41.0 34-35 38.38024087817953 40.0 38.0 41.0 34.0 41.0 36-37 38.21930915092071 40.0 38.0 41.0 34.0 41.0 38-39 37.732734444573225 40.0 36.0 41.0 33.5 41.0 40-41 37.504636857521035 40.0 35.0 41.0 33.0 41.0 42-43 37.35343318845774 40.0 35.0 41.0 33.0 41.0 44-45 37.190759778071005 40.0 35.0 41.0 33.0 41.0 46-47 37.049747069249484 40.0 35.0 41.0 33.0 41.0 48-49 36.874145812185304 40.0 35.0 41.0 32.5 41.0 50-51 36.59176308654711 40.0 35.0 41.0 31.5 41.0 52-53 35.99858376103737 39.0 35.0 41.0 30.0 41.0 54-55 35.0821121615061 39.0 35.0 41.0 25.0 41.0 56-57 34.28658008916923 39.0 35.0 41.0 17.5 41.0 58-59 33.948844838203925 39.0 34.0 41.0 11.0 41.0 60-61 33.661289963326695 38.0 34.0 41.0 7.5 41.0 62-63 33.395430231824314 38.0 34.0 40.0 6.5 41.0 64-65 33.14701610185536 37.0 34.0 40.0 2.0 41.0 66-67 32.85092553598262 36.5 33.5 40.0 2.0 41.0 68-69 32.53217741532987 36.0 33.0 39.5 2.0 41.0 70-71 32.23804673279219 36.0 33.0 39.0 2.0 41.0 72-73 31.91851552500661 35.0 33.0 39.0 2.0 41.0 74-75 31.635234837457347 35.0 33.0 38.5 2.0 40.0 76-77 31.38698281085589 35.0 33.0 37.0 2.0 39.5 78-79 31.08056640484017 35.0 33.0 37.0 2.0 39.0 80-81 30.771906847073005 35.0 33.0 36.5 2.0 39.0 82-83 30.462039041750486 35.0 32.5 36.0 2.0 38.0 84-85 30.176079833227398 35.0 32.0 36.0 2.0 37.0 86-87 29.988187696725635 35.0 32.0 35.0 2.0 37.0 88-89 29.810370305137937 35.0 32.0 35.0 2.0 36.5 90-91 29.620978918725164 35.0 32.0 35.0 2.0 36.0 92-93 29.459594291981574 35.0 32.0 35.0 2.0 36.0 94-95 29.291045871953187 35.0 32.0 35.0 2.0 36.0 96-97 29.130508884955695 35.0 32.0 35.0 2.0 36.0 98-99 28.977701300003012 35.0 32.0 35.0 2.0 35.0 100 28.873186700594257 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 3.8564873321896123 1101 2 3.9198450038489696 1101 3 3.2879141000393375 1101 4 1.8176328222881182 1101 5 0.9873608708542179 1101 6 0.8973841780573366 1101 7 0.5183855255151641 1101 8 0.5573967150727697 1101 9 0.6262381807636217 1101 10-11 0.4445278641285313 1101 12-13 0.39786964216542486 1101 14-15 0.5167404457973745 1101 16-17 0.5201758126625933 1101 18-19 0.4221694029212131 1101 20-21 0.4747994648537315 1101 22-23 0.45503124807668627 1101 24-25 0.5174367326864768 1101 26-27 0.4353299431081652 1101 28-29 0.44867135263673674 1101 30-31 0.4929137360308289 1101 32-33 0.4967417199080373 1101 34-35 0.4965882656746956 1101 36-37 0.40829909662615904 1101 38-39 0.39691382569026956 1101 40-41 0.36386316503994465 1101 42-43 0.44208729958231174 1101 44-45 0.47299886439442673 1101 46-47 0.45368220907530343 1101 48-49 0.3899952895756016 1101 50-51 0.41870285523484796 1101 52-53 0.42071956967496504 1101 54-55 0.4866759002563228 1101 56-57 0.4826153813483458 1101 58-59 0.4154322466317595 1101 60-61 0.37035281565643174 1101 62-63 0.4139745543979316 1101 64-65 0.4590099616372427 1101 66-67 0.4234392305628063 1101 68-69 0.39761938115270823 1101 70-71 0.40456140538786656 1101 72-73 0.3695902070935979 1101 74-75 0.30427407075688606 1101 76-77 0.357299950914161 1101 78-79 0.37638719627295103 1101 80-81 0.38154152601727276 1101 82-83 0.3843547022205307 1101 84-85 0.3779050102005961 1101 86-87 0.36471255826185356 1101 88-89 0.3589885855127193 1101 90-91 0.4040842174220387 1101 92-93 0.40002838649742145 1101 94-95 0.4519779984313921 1101 96-97 0.437620344813066 1101 98-99 0.4423732400346303 1101 100 0.4907365281340752 1102 1 2.8819907083440626 1102 2 3.479232248434595 1102 3 4.15153665392447 1102 4 2.1200188357952854 1102 5 1.137236319313189 1102 6 0.9162603005482595 1102 7 0.5305944532850617 1102 8 0.5630893267078463 1102 9 0.6445600235347655 1102 10-11 0.44444925899228593 1102 12-13 0.41566044800357105 1102 14-15 0.5462624119956061 1102 16-17 0.552298736268547 1102 18-19 0.47208158934932953 1102 20-21 0.5132159516141925 1102 22-23 0.41108173655297975 1102 24-25 0.4958401222562969 1102 26-27 0.47053117473997474 1102 28-29 0.46006254400895585 1102 30-31 0.4710368908408782 1102 32-33 0.49503760063708313 1102 34-35 0.5011365269936405 1102 36-37 0.43284629060369895 1102 38-39 0.43594388300320475 1102 40-41 0.458326931867596 1102 42-43 0.5103834839485302 1102 44-45 0.47211958471544335 1102 46-47 0.5132292001551093 1102 48-49 0.5242899922208082 1102 50-51 0.5394417722476135 1102 52-53 0.5017783407773067 1102 54-55 0.3384247598965757 1102 56-57 0.34060470311017355 1102 58-59 0.34956396244922416 1102 60-61 0.4249719723524379 1102 62-63 0.3605269015522623 1102 64-65 0.41586655181595944 1102 66-67 0.34177113663591285 1102 68-69 0.3548725796474912 1102 70-71 0.3838266225937943 1102 72-73 0.3984299207921218 1102 74-75 0.44974906818696425 1102 76-77 0.4457035579250892 1102 78-79 0.4880741341495636 1102 80-81 0.4945114816869527 1102 82-83 0.4612753882191889 1102 84-85 0.41410460864753773 1102 86-87 0.40004936247071754 1102 88-89 0.41074424151762656 1102 90-91 0.47982994108624766 1102 92-93 0.5028913391518017 1102 94-95 0.49992017910387787 1102 96-97 0.525197595819769 1102 98-99 0.506854397184533 1102 100 0.5931792040260149 1103 1 2.676941909084835 1103 2 3.2814807140014075 1103 3 3.100285737765798 1103 4 1.7475505062898975 1103 5 0.904091050407466 1103 6 0.7800982700048777 1103 7 0.4141384791779785 1103 8 0.4780212625591318 1103 9 0.5513310954635884 1103 10-11 0.40019810326217 1103 12-13 0.34624270578694905 1103 14-15 0.4420484758308376 1103 16-17 0.4750855547162942 1103 18-19 0.41100048291791325 1103 20-21 0.41806424488721206 1103 22-23 0.4016517341310788 1103 24-25 0.4119418131320316 1103 26-27 0.3515160848818297 1103 28-29 0.34966639522686904 1103 30-31 0.40324043322621606 1103 32-33 0.3926803649901842 1103 34-35 0.3445440598454894 1103 36-37 0.3684316909616925 1103 38-39 0.3303234401925508 1103 40-41 0.31702447790416954 1103 42-43 0.3343860702934691 1103 44-45 0.2848844789101861 1103 46-47 0.2695122498093241 1103 48-49 0.2584835193289834 1103 50-51 0.28688940923002093 1103 52-53 0.18060380968895373 1103 54-55 0.16831385286224787 1103 56-57 0.2061955197977241 1103 58-59 0.1592181381623874 1103 60-61 0.10744841334430077 1103 62-63 0.07579607423878798 1103 64-65 0.13610982202955313 1103 66-67 0.19757264611585157 1103 68-69 0.25066693707911725 1103 70-71 0.26093838830343685 1103 72-73 0.24235288756414874 1103 74-75 0.24925706760512156 1103 76-77 0.23637974147323604 1103 78-79 0.20120609942827627 1103 80-81 0.22893393824566033 1103 82-83 0.24053499110817356 1103 84-85 0.22545743512177197 1103 86-87 0.22968589360122138 1103 88-89 0.24913643584311984 1103 90-91 0.24453031012335202 1103 92-93 0.25247719623379083 1103 94-95 0.27471909300243524 1103 96-97 0.2654620354817858 1103 98-99 0.27749143600535575 1103 100 0.28398514831019384 1104 1 2.3692968448132703 1104 2 3.177096233106603 1104 3 1.5812448358268085 1104 4 1.0722020154890401 1104 5 0.6067933215483876 1104 6 0.5745876469975499 1104 7 0.2966382829948202 1104 8 0.3873438127812321 1104 9 0.43110282405730516 1104 10-11 0.33140108999451456 1104 12-13 0.30231388212288834 1104 14-15 0.40320086604207006 1104 16-17 0.466574698530259 1104 18-19 0.37521459979237193 1104 20-21 0.3766167486308021 1104 22-23 0.3255066416532628 1104 24-25 0.3657209118394533 1104 26-27 0.2916188449381423 1104 28-29 0.35565357085364013 1104 30-31 0.341929746578856 1104 32-33 0.3113285749506005 1104 34-35 0.34801847148597886 1104 36-37 0.3056694940506688 1104 38-39 0.2980683724602571 1104 40-41 0.35748295190839485 1104 42-43 0.3333843981231084 1104 44-45 0.2544749773351782 1104 46-47 0.3100076055472627 1104 48-49 0.34572574157277103 1104 50-51 0.4498294946303574 1104 52-53 0.3528153659048172 1104 54-55 0.28896697767917345 1104 56-57 0.25936310832972964 1104 58-59 0.21881807438929712 1104 60-61 0.23562041315226168 1104 62-63 0.23479342089 1104 64-65 0.21882838976677021 1104 66-67 0.21638990275427972 1104 68-69 0.23729541330726533 1104 70-71 0.136660392231164 1104 72-73 0.10370315751253045 1104 74-75 0.2083502875046115 1104 76-77 0.19865183765869787 1104 78-79 0.20146161902090398 1104 80-81 0.24889337306587578 1104 82-83 0.22214976638067085 1104 84-85 0.18124513073108872 1104 86-87 0.22021319716707666 1104 88-89 0.20987971281869022 1104 90-91 0.2403402107599497 1104 92-93 0.22406507734255854 1104 94-95 0.17811395285307086 1104 96-97 0.18680428406890925 1104 98-99 0.19916403567331642 1104 100 0.1787900746663098 1105 1 1.350535678106695 1105 2 2.583124247777171 1105 3 1.3709131906552905 1105 4 0.9443273660401275 1105 5 0.6027766748909542 1105 6 0.5772748043674554 1105 7 0.2767055313451223 1105 8 0.35976093069808 1105 9 0.5099928310868833 1105 10-11 0.31612189449498374 1105 12-13 0.3261516477286506 1105 14-15 0.4604880295425815 1105 16-17 0.4303876791677155 1105 18-19 0.27943078668184285 1105 20-21 0.37358452129912934 1105 22-23 0.3211984475621179 1105 24-25 0.36896863822308035 1105 26-27 0.33173257936523015 1105 28-29 0.358825644045929 1105 30-31 0.33008489325016654 1105 32-33 0.3313848837429063 1105 34-35 0.3162308836540504 1105 36-37 0.3669549020809981 1105 38-39 0.312498922315136 1105 40-41 0.2031203555609693 1105 42-43 0.27614257842726175 1105 44-45 0.3201158820311889 1105 46-47 0.3150132763579023 1105 48-49 0.27637082016686065 1105 50-51 0.2800099358250634 1105 52-53 0.24579587690045912 1105 54-55 0.2883362365933664 1105 56-57 0.26899872695874194 1105 58-59 0.1986812842096981 1105 60-61 0.19369100213306467 1105 62-63 -0.1265105507805515 1105 64-65 0.03155655209423003 1105 66-67 0.14170058084054205 1105 68-69 0.24467361837026402 1105 70-71 0.2338359113464179 1105 72-73 0.20444573404957467 1105 74-75 0.17056331397040836 1105 76-77 0.19703947794441845 1105 78-79 0.17630508581542514 1105 80-81 0.17852839650376495 1105 82-83 0.21149854274743163 1105 84-85 0.21308407613488 1105 86-87 0.2768705474201525 1105 88-89 0.22146777828806563 1105 90-91 0.24575048782761044 1105 92-93 0.17626726047063457 1105 94-95 0.20151243853838352 1105 96-97 0.20564261599971445 1105 98-99 0.243491273131486 1105 100 0.29426997191405135 1106 1 1.8934116153201899 1106 2 2.8624573963470326 1106 3 1.2000039206490811 1106 4 0.9101582483159021 1106 5 0.560669207425704 1106 6 0.5469457695673725 1106 7 0.2860999666891999 1106 8 0.3284610529090983 1106 9 0.371695585461822 1106 10-11 0.2719736413683975 1106 12-13 0.20927454230757547 1106 14-15 0.23103225256767956 1106 16-17 0.2922537512521828 1106 18-19 0.09620262701186277 1106 20-21 0.2172423681337179 1106 22-23 0.22401373720033035 1106 24-25 0.24202462680145231 1106 26-27 0.23969968113237883 1106 28-29 0.19581787443993193 1106 30-31 0.2200513305366485 1106 32-33 0.192376469690835 1106 34-35 0.22481321438320379 1106 36-37 0.26642596497340065 1106 38-39 0.19689103221537607 1106 40-41 0.10713789966330722 1106 42-43 0.13960694594946688 1106 44-45 0.13631715384623533 1106 46-47 0.053766177796291004 1106 48-49 0.03969656738562577 1106 50-51 0.07248846064936032 1106 52-53 -0.0036367148031217766 1106 54-55 -0.0021798521388873837 1106 56-57 0.016835289041651436 1106 58-59 0.019945583900501163 1106 60-61 -0.08846529488263855 1106 62-63 -0.02411112205266619 1106 64-65 -0.2514524672887646 1106 66-67 -0.2878306597682396 1106 68-69 -0.07688625119499193 1106 70-71 0.024496893117799345 1106 72-73 -0.059816570768173705 1106 74-75 0.02546933264837392 1106 76-77 -0.02944807866522936 1106 78-79 -0.023183829330442052 1106 80-81 0.023921859216045505 1106 82-83 -0.028568413339602472 1106 84-85 -0.030634840432490762 1106 86-87 0.010000346778468838 1106 88-89 -0.013630002451439083 1106 90-91 -0.032995396733067395 1106 92-93 -0.014104603042266461 1106 94-95 0.024347545728222286 1106 96-97 0.06915557933545813 1106 98-99 0.10348739023654119 1106 100 0.10281355717126317 1107 1 1.741041781505917 1107 2 1.0725276109458974 1107 3 0.6042935900025057 1107 4 0.42664466139503077 1107 5 0.23022026351138436 1107 6 0.34351887831375194 1107 7 0.12763208898144995 1107 8 0.20975618210231062 1107 9 0.3080878689012181 1107 10-11 0.1838168098149282 1107 12-13 0.15443830591890872 1107 14-15 0.2237504214393553 1107 16-17 0.24299815670201497 1107 18-19 0.2019138371022393 1107 20-21 0.24922727765547137 1107 22-23 0.13613452564842277 1107 24-25 0.0971297009006662 1107 26-27 0.2320966222227625 1107 28-29 0.2905636107165961 1107 30-31 0.2779811684128788 1107 32-33 0.2040142199861279 1107 34-35 0.19462831010896053 1107 36-37 0.23078310370055277 1107 38-39 0.2557490841550347 1107 40-41 0.2502421735172504 1107 42-43 0.154940485943996 1107 44-45 0.13134878224090585 1107 46-47 0.17372583513389372 1107 48-49 0.16170919153418595 1107 50-51 0.16671837398283884 1107 52-53 0.15829476243411023 1107 54-55 0.06764355214433948 1107 56-57 0.1984309596515672 1107 58-59 0.13620841904268133 1107 60-61 0.18076585744626072 1107 62-63 0.11218924493292093 1107 64-65 0.12677074283659806 1107 66-67 0.06691431610536114 1107 68-69 0.07444033633468905 1107 70-71 0.10712956644718474 1107 72-73 0.0725454913866379 1107 74-75 0.13160593116381492 1107 76-77 0.1119303474607598 1107 78-79 0.08937404511705438 1107 80-81 0.07725417381981714 1107 82-83 0.07589730268556494 1107 84-85 -0.015733794866125805 1107 86-87 0.066104124062484 1107 88-89 0.09597377896255921 1107 90-91 0.1253001755832095 1107 92-93 0.08889770148919851 1107 94-95 0.1421978741105967 1107 96-97 0.15245858562420977 1107 98-99 0.17290824248075154 1107 100 0.16085429728395795 1108 1 1.4932702668981612 1108 2 -0.4882388038274108 1108 3 0.2943814456745848 1108 4 0.083688588097516 1108 5 0.07758924089765884 1108 6 0.08992694668486223 1108 7 0.019878532614903577 1108 8 0.04623076709605556 1108 9 0.0963134603442839 1108 10-11 0.05029454465640981 1108 12-13 0.1044308244991825 1108 14-15 0.10699383368088178 1108 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-0.5434304534576171 2215 8 -0.3302465489118447 2215 9 -0.3008732648321697 2215 10-11 -0.3764879732842985 2215 12-13 -0.3184142025570864 2215 14-15 -0.5126650073066159 2215 16-17 -0.5004857553801472 2215 18-19 -0.5100131381338358 2215 20-21 -0.4727790520364934 2215 22-23 -0.4757889998272802 2215 24-25 -0.4906772770140009 2215 26-27 -0.6241063274940117 2215 28-29 -0.6306389894096966 2215 30-31 -0.6587348693883683 2215 32-33 -0.7271248251807378 2215 34-35 -0.7367116296108591 2215 36-37 -0.7896878925082902 2215 38-39 -0.8433546407814063 2215 40-41 -0.8133823809948382 2215 42-43 -0.8305765561675926 2215 44-45 -0.9788083976277875 2215 46-47 -0.8898250451216612 2215 48-49 -0.9002437796490028 2215 50-51 -0.9499571641114883 2215 52-53 -0.9830893198214739 2215 54-55 -1.0845910601339668 2215 56-57 -1.0790736715917575 2215 58-59 -1.1591287415989058 2215 60-61 -1.2341924549036491 2215 62-63 -1.091129618356824 2215 64-65 -1.155660262509592 2215 66-67 -1.1552319328404188 2215 68-69 -1.0774380591136534 2215 70-71 -1.0383579863965373 2215 72-73 -1.1372102297705418 2215 74-75 -1.134016075213868 2215 76-77 -1.0673853636819608 2215 78-79 -1.086471962009572 2215 80-81 -1.0444398427341604 2215 82-83 -1.1238860084332067 2215 84-85 -1.1280720298985791 2215 86-87 -1.1293487964356181 2215 88-89 -1.1558313464547822 2215 90-91 -1.0927234343627 2215 92-93 -1.1591668334310476 2215 94-95 -1.134598310354754 2215 96-97 -1.1840163122654666 2215 98-99 -1.199193017455169 2215 100 -1.1754593349213067 2216 1 -3.1662682229069716 2216 2 -4.436391441682542 2216 3 -5.883377262995722 2216 4 -3.2463557243035552 2216 5 -1.63945246407215 2216 6 -0.7955104029455811 2216 7 -0.6302048405374663 2216 8 -0.4282126804260642 2216 9 -0.2791124331698924 2216 10-11 -0.44015895383091674 2216 12-13 -0.3667344331393352 2216 14-15 -0.5427013376685679 2216 16-17 -0.4478556030313925 2216 18-19 -0.47637344918618396 2216 20-21 -0.43916438953221615 2216 22-23 -0.4318494010646745 2216 24-25 -0.5063477241564698 2216 26-27 -0.5826999491988047 2216 28-29 -0.5656889294528256 2216 30-31 -0.7361972084670612 2216 32-33 -0.8068124035535433 2216 34-35 -0.7879860199764792 2216 36-37 -0.7760973357279752 2216 38-39 -0.9305326398227436 2216 40-41 -0.998485285307865 2216 42-43 -1.070250399859134 2216 44-45 -1.0759263168202295 2216 46-47 -1.1293896921246898 2216 48-49 -1.1022157488696323 2216 50-51 -1.1313897260037393 2216 52-53 -1.1408590932090945 2216 54-55 -1.1141400487406088 2216 56-57 -1.1865821366695073 2216 58-59 -1.2362717446695726 2216 60-61 -1.2095933638154435 2216 62-63 -1.2231294137055286 2216 64-65 -1.1716578416482548 2216 66-67 -1.1309413602760934 2216 68-69 -1.115916510905457 2216 70-71 -1.083195128027512 2216 72-73 -1.1264904993859375 2216 74-75 -1.1760462823160118 2216 76-77 -1.129679757707553 2216 78-79 -1.1773048612487784 2216 80-81 -1.1232022002783353 2216 82-83 -1.110243830759483 2216 84-85 -1.0921258295812848 2216 86-87 -1.1467106558857374 2216 88-89 -1.090505757711881 2216 90-91 -1.1112749175622234 2216 92-93 -1.1798606189432057 2216 94-95 -1.1454530478745788 2216 96-97 -1.1148192269890025 2216 98-99 -1.1796057689574901 2216 100 -1.1987001182178005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5135.0 3 129.0 4 264.0 5 546.0 6 1182.0 7 1935.0 8 2554.0 9 3667.0 10 5653.0 11 9417.0 12 9467.0 13 12232.0 14 13201.0 15 14685.0 16 17606.0 17 22820.0 18 33352.0 19 57755.0 20 109434.0 21 129470.0 22 58282.0 23 34693.0 24 33129.0 25 34360.0 26 36574.0 27 42556.0 28 51337.0 29 62300.0 30 74335.0 31 91856.0 32 116401.0 33 153013.0 34 213550.0 35 373723.0 36 529941.0 37 892257.0 38 1165851.0 39 430690.0 40 3406.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 16.56586869273448 43.76124939014767 25.035539047567966 14.63734286954988 2 17.70413131531273 43.029860871757016 23.357687118524574 15.908320694405683 3 18.8145785882631 23.61309885854313 20.93476001520485 36.63756253798892 4 36.584708908961844 24.922237818426904 21.585630794525112 16.90742247808614 5 16.323492469307368 43.324159471743975 22.537834960415033 17.814513098533627 6 38.13792793376212 22.91163869288012 22.956372277469494 15.994061095888265 7 17.397938065236236 22.75886945637708 42.79495300538469 17.048239473001995 8 37.589956025852395 23.26160637425089 22.507371990930462 16.641065608966255 9 37.17795361203839 24.377026034295792 22.416792093975406 16.02822825969042 10-11 16.004758331927473 45.047504948689955 22.70945466859761 16.238282050784964 12-13 15.870703384248088 23.95452608688658 33.08035789783693 27.094412631028398 14-15 16.096678778359326 34.20726503570605 22.80245786652994 26.89359831940468 16-17 26.753026238884264 23.64170371051721 33.18516824308411 16.42010180751442 18-19 16.371347054235333 23.808478377349417 33.15929563818199 26.660878930233267 20-21 26.876387726506458 34.342825523567065 22.72866577379197 16.052120976134507 22-23 26.54340554838992 23.932479203952848 33.30995277553551 16.214162472121725 24-25 16.226081217645966 34.11359745911275 22.841503908264073 26.818817414977207 26-27 16.50460266708741 28.247377178423022 39.04973602919225 16.19828412529732 28-29 25.86481693908942 23.66438812435627 34.27299486008042 16.197800076473886 30-31 23.490298304839357 24.869684531848595 34.84272996481851 16.79728719849354 32-33 19.02918967036376 26.84138545231346 35.978394124693516 18.151030752629268 34-35 18.843518409947645 26.28775168261077 36.416262585355184 18.4524673220864 36-37 18.718533383012016 26.440904351384344 36.71454306973623 18.126019195867414 38-39 19.26935590372685 26.24392966407688 36.224200782287134 18.26251364990913 40-41 18.54477003559888 27.235181046490993 35.99599779429046 18.224051123619667 42-43 18.75528735303827 26.503629613487856 36.29140120540563 18.449681828068247 44-45 18.7121807221473 26.87557062474108 35.81322088990079 18.599027763210827 46-47 19.10790562921591 26.51986230632573 35.92535136576754 18.44688069869082 48-49 18.710136542108994 26.87648709610547 35.84868258884416 18.564693772941375 50-51 19.172126890011832 27.22112310384884 35.19268117172737 18.414068834411964 52-53 19.464336081901166 27.07782601364504 34.65806705776737 18.79977084668643 54-55 19.78743368904396 28.006016751492506 32.94784007345832 19.258709486005205 56-57 20.359674912783113 28.74014781754114 31.295594864026217 19.60458240564953 58-59 20.95023628182957 29.32335727588496 30.22824402942738 19.49816241285809 60-61 20.709392275677764 29.594803009960525 29.955206612490787 19.740598101870923 62-63 19.978024673048942 29.680160398847853 30.089810277683444 20.25200465041976 64-65 20.341021362435363 29.634165458321903 30.38000466149502 19.644808517747713 66-67 20.230784311278725 29.092174192117238 30.81988017952137 19.857161317082674 68-69 20.483257654812515 29.227769711658492 30.637152610736234 19.65182002279276 70-71 19.491575761838185 29.825862022382278 30.8437499806644 19.838812235115142 72-73 19.566352408507626 29.25559456211666 31.05141685320621 20.1266361761695 74-75 19.79631862591073 29.48925077415855 30.496087417295303 20.21834318263542 76-77 20.231960397407743 29.19085584765727 30.606243255241512 19.970940499693473 78-79 20.19155868061048 29.625464325776598 29.906498591271014 20.27647840234191 80-81 20.435178413579948 29.867048296463487 29.686792977384762 20.010980312571796 82-83 20.462045115560777 29.884882640804634 29.66452478847956 19.988547455155025 84-85 20.346502257403152 30.22018420145717 29.21882569663331 20.21448784450637 86-87 20.353142102423565 30.43367250132406 29.164648955354888 20.04853644089749 88-89 21.18540300620971 30.045989675164275 28.810265933301256 19.958341385324758 90-91 20.74991704001239 30.56058265238084 28.663000546741895 20.026499760864873 92-93 20.389857863117676 30.69375837008428 28.644350291720027 20.27203347507802 94-95 20.48653766176528 30.902150573227367 28.581728763848602 20.029583001158755 96-97 20.44024262223405 30.360715987204788 28.848276544798697 20.350764845762466 98-99 20.925786518939564 30.521111335690037 28.072074710533805 20.48102743483659 100 20.52177550016994 31.09604285803852 28.144314248284918 20.23786739350662 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 98827.0 1 56434.0 2 10058.0 3 5067.5 4 3654.5 5 3103.5 6 2908.0 7 3018.0 8 3019.0 9 2946.0 10 3189.0 11 5377.0 12 38315.5 13 42402.0 14 13995.5 15 13497.5 16 16054.5 17 18662.5 18 21326.0 19 24186.0 20 27365.0 21 31313.0 22 35808.5 23 41029.0 24 46510.5 25 52684.5 26 61004.5 27 69631.0 28 76845.0 29 82991.0 30 88414.5 31 95082.0 32 101675.5 33 108744.0 34 117667.0 35 125041.0 36 132656.5 37 138858.5 38 141327.5 39 141944.0 40 143122.0 41 144891.5 42 145108.5 43 145218.5 44 145475.0 45 144880.0 46 143822.5 47 146297.0 48 146516.0 49 146984.5 50 162055.0 51 156649.5 52 129591.0 53 115894.0 54 109462.5 55 106625.0 56 98423.0 57 86313.0 58 81007.5 59 68068.0 60 50938.5 61 41039.5 62 32304.0 63 24812.5 64 18843.0 65 14677.0 66 11276.0 67 8752.5 68 7043.5 69 5347.5 70 3855.5 71 2902.0 72 2270.5 73 1607.5 74 1073.0 75 741.5 76 543.0 77 356.0 78 229.5 79 158.0 80 91.5 81 57.5 82 44.5 83 32.0 84 26.0 85 23.0 86 16.0 87 11.0 88 13.0 89 13.0 90 9.0 91 8.5 92 10.0 93 5.5 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026254145907054962 2 0.013219880224997824 3 0.006290270621878839 4 0.0 5 0.004124767620904158 6 0.001072439581435081 7 2.2686221914972865E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011961826100622057 26-27 0.004341317921001626 28-29 9.074488765989146E-4 30-31 8.971369575466543E-4 32-33 0.005403445583384446 34-35 0.008548580894323867 36-37 0.0054962528548547895 38-39 0.005826234264527123 40-41 0.00889918614210072 42-43 0.010610964704775945 44-45 0.009352910580400177 46-47 0.012477422053235076 48-49 0.016560941997930193 50-51 0.06094344159885892 52-53 0.009806635018699633 54-55 0.006073720321781372 56-57 0.004392877516262927 58-59 0.008589828570532908 60-61 0.014869787273359488 62-63 0.012240247915033087 64-65 0.010889386519186976 66-67 0.008692947761055511 68-69 0.005949977293154247 70-71 0.0038979054017544285 72-73 0.002959520767998733 74-75 0.0021964387581314636 76-77 0.0017633381579365273 78-79 0.001258054124375768 80-81 0.001649907048361663 82-83 0.0011136872576441225 84-85 9.280727147034354E-4 86-87 6.187151431356236E-4 88-89 0.001258054124375768 90-91 7.218343336582275E-4 92-93 3.40293328724593E-4 94-95 5.671555478743216E-4 96-97 0.0014024209911074135 98-99 0.0036710431826047 100 1.2374302862712473E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4848758.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.461609567407436 #Duplication Level Percentage of deduplicated Percentage of total 1 85.45551127044052 52.52233269087001 2 10.194855080479462 12.531844051054575 3 2.31581816979032 4.270017365422821 4 0.7328961843806158 1.8017991655137622 5 0.3259904993001808 1.001795039533596 6 0.19478902523669342 0.7183228210268114 7 0.1399133066356556 0.6019507918007934 8 0.09280883046939713 0.4563344082174234 9 0.06584455464176868 0.364222107857899 >10 0.3515128793133496 4.3263521934678435 >50 0.1052194739832975 4.660580614215986 >100 0.020630854438729775 2.1388051026653634 >500 0.002739757531985256 1.1807622691776976 >1k 0.001102585018452563 1.1553392397249567 >5k 1.336466689032768E-4 0.5777414935819234 >10k+ 2.3388167058073438E-4 11.691800645868632 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 384968 7.939517707421158 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92037 1.8981561876257795 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 27007 0.5569879956887929 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 21522 0.4438662436854964 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17541 0.3617627441913991 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 14018 0.28910496254917234 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 12257 0.25278638364711126 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA 8718 0.17979862059521223 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 7169 0.1478522953713095 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6240 0.1286927497722097 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 6007 0.12388739549385637 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.66201200389873E-4 2.0623838104520787E-5 0.0 0.0 0.0 2 9.074488765989146E-4 2.0623838104520787E-5 0.0 0.0 0.0 3 9.280727147034354E-4 2.0623838104520787E-5 0.0 0.0 0.0 4 9.693203909124769E-4 4.1247676209041574E-5 0.0 2.0623838104520787E-5 0.0 5 9.693203909124769E-4 4.1247676209041574E-5 0.0 2.0623838104520787E-5 0.0 6 9.693203909124769E-4 4.1247676209041574E-5 0.0 2.0623838104520787E-5 0.0 7 9.899442290169978E-4 8.249535241808315E-5 0.0 2.0623838104520787E-5 0.0 8 9.899442290169978E-4 8.249535241808315E-5 0.0 2.0623838104520787E-5 0.0 9 9.899442290169978E-4 1.2374302862712473E-4 0.0 2.0623838104520787E-5 0.0 10-11 9.899442290169978E-4 2.1655030009746826E-4 0.0 2.0623838104520787E-5 0.0 12-13 9.899442290169978E-4 2.4748605725424946E-4 0.0 2.0623838104520787E-5 0.0 14-15 9.899442290169978E-4 2.88733733463291E-4 0.0 2.0623838104520787E-5 0.0 16-17 9.899442290169978E-4 3.196694906200722E-4 0.0 2.0623838104520787E-5 0.0 18-19 0.0010311919052260394 3.9185292398589495E-4 0.0 2.0623838104520787E-5 0.0 20-21 0.00105181574333056 4.434125192471969E-4 0.0 2.0623838104520787E-5 0.0 22-23 0.001072439581435081 4.8466019545623847E-4 0.0 2.0623838104520787E-5 0.0 24-25 0.0010930634195396016 4.949721145084989E-4 0.0 2.0623838104520787E-5 0.0 26-27 0.0011136872576441225 5.155959526130197E-4 0.0 3.093575715678118E-5 0.0 28-29 0.0011755587719576848 5.77467466926582E-4 0.0 4.1247676209041574E-5 0.0 30-31 0.001237430286271247 6.187151431356236E-4 0.0 4.1247676209041574E-5 0.0 32-33 0.0012786779624802888 6.496509002924047E-4 0.0 4.1247676209041574E-5 0.0 34-35 0.0014024209911074135 7.012104955537068E-4 0.0 4.1247676209041574E-5 0.0 36-37 0.0015364759387867986 7.321462527104879E-4 0.0 4.1247676209041574E-5 0.0 38-39 0.0017117785626752254 7.424581717627483E-4 0.0 4.1247676209041574E-5 0.0 40-41 0.002041759972347558 7.630820098672691E-4 0.0 4.1247676209041574E-5 0.0 42-43 0.002567667844012838 7.630820098672691E-4 0.0 4.1247676209041574E-5 0.0 44-45 0.0031245114728348992 7.733939289195294E-4 0.0 4.1247676209041574E-5 0.0 46-47 0.0037741623731273035 8.249535241808315E-4 0.0 4.1247676209041574E-5 0.0 48-49 0.004702235087830739 8.352654432330919E-4 0.0 4.1247676209041574E-5 0.0 50-51 0.006331518298087882 8.558892813376126E-4 0.0 5.155959526130197E-5 0.0 52-53 0.008641388165794208 8.971369575466541E-4 0.0 7.218343336582276E-5 0.0 54-55 0.011209056009807048 9.486965528079562E-4 0.0 1.753026238884267E-4 0.0 56-57 0.014374815158850989 9.693203909124769E-4 0.0 6.393389812401444E-4 0.0 58-59 0.018231472884396374 9.899442290169976E-4 0.0 0.0011136872576441225 0.0 60-61 0.02360398271062404 0.0010311919052260394 0.0 0.0011961826100622057 0.0 62-63 0.030636711504265626 0.00105181574333056 0.0 0.0011961826100622057 0.0 64-65 0.04030929157528587 0.0010621276623828204 0.0 0.0011961826100622057 0.0 66-67 0.05300326392861842 0.0010827515004873413 0.0 0.0012168064481667264 0.0 68-69 0.07092537924144698 0.0011033753385918622 0.0 0.0012168064481667264 0.0 70-71 0.09427156397576451 0.0011136872576441225 0.0 0.0012168064481667264 0.0 72-73 0.12583634819473358 0.0011136872576441225 0.0 0.0012168064481667264 0.0 74-75 0.16565066765551095 0.001154934933853164 0.0 0.0012168064481667264 0.0 76-77 0.21608626374011655 0.001154934933853164 0.0 0.0012168064481667264 0.0 78-79 0.27795777805367894 0.001154934933853164 0.0 0.0012374302862712472 0.0 80-81 0.35899914988539333 0.0011652468529054244 0.0 0.0012374302862712472 0.0 82-83 0.4593134984257824 0.0012168064481667264 0.0 0.0012374302862712472 0.0 84-85 0.5853663969206135 0.0012271183672189867 0.0 0.0012374302862712472 0.0 86-87 0.7343220676305149 0.001258054124375768 0.0 0.0012374302862712472 0.0 88 0.8643863026366752 0.0012786779624802888 0.0 0.0012374302862712472 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 6825 0.0 89.90074 1 AACGAGT 104030 0.0 79.72968 1 ACGAGTG 113100 0.0 79.38056 2 CGAGTGG 117280 0.0 78.78522 3 GTGGAAT 121755 0.0 78.40209 6 ATCGAGT 2940 0.0 78.04191 1 GAATCAC 122880 0.0 78.03534 9 TGGAATC 123535 0.0 77.42457 7 AGTGGAA 123280 0.0 77.410965 5 GAGTGGA 123665 0.0 77.234566 4 GGAATCA 123865 0.0 77.19095 8 TCGAGTG 3465 0.0 75.153625 2 GACGAGT 1655 0.0 65.62501 1 ATGAGTG 5545 0.0 55.863384 2 TGAGTGG 5980 0.0 55.722454 3 AATGAGT 5500 0.0 51.37682 1 ACGATTG 840 0.0 45.88576 2 AGTACTT 62370 0.0 42.733803 20-21 GAGTACT 61765 0.0 42.566525 20-21 GTACTTT 62385 0.0 41.540844 22-23 >>END_MODULE