FastQCFastQC Report
Thu 9 Feb 2017
SRR2132002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096089
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT97060.3134922800991832No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC95720.30916423914170427No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG94650.3057082661383442No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA86490.27935243463608445No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT85190.27515358893106756No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC83770.2705671574686645No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA83270.2689522168128888No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA81460.26310613163898067No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC81120.2620079719930532No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA80050.25855199898969317No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC79560.2569693571470329No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA73580.23765466690395526No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT68610.22160215678554462No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA67960.21950273393303615No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA65750.21236469623450746No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC64180.2072937825753717No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA62340.20135080096211705No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA60940.19682896712594503No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT59090.19085368669957486No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG58150.1878175982667165No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC56650.18297277629938932No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG53440.1726048572893092No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG52880.17079612375484038No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT51780.1672432543121338No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT49700.1605251011841068No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT49620.1602667106791827No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA49480.15981452729556547No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG49180.15884556290210003No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC48530.1567461400495916No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG48410.15635855429220544No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC47300.15277338603638332No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT46970.15170752520357134No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG46840.15128764063306965No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG44070.14234086940007215No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC44010.14214707652137906No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG43570.14072592874429643No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT41440.13384628155069186No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG41430.13381398273757636No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC40360.1303580097342163No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG39680.1281616904423613No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG39160.12648215216035458No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC39090.12625606046854598No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG38850.1254808889537736No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT38700.1249964067570409No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG38690.12496410794392539No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA38670.12489951031769435No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG38180.12331686847503415No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA37730.121863421884836No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC37170.1200546883503672No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG36800.11885963226509315No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC36740.11866583938640006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36690.1185043453208225No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGC36690.1185043453208225No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT36550.11805216193720529No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA36220.1169863011043933No Hit
GCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCC35530.11475768299942282No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT35500.11466078656007628No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC35490.11462848774696076No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG35440.11446699368138319No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG34960.1129166506518385No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT34660.11194768625837306No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA33650.10868550613370612No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG33570.10842711562878199No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC33420.10794263343204928No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCG33370.10778113936647171No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAG32970.10648918684185113No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC32920.10632769277627355No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC32210.10403447704507203No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC31870.10293631739914454No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA31770.10261332926798938No Hit
GGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAAC31530.10183815775321704No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG31190.10073999810728954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.034.0256271
GTATCAA40850.030.8604531
CGACGAG2000.026.39975724
TAGACCG651.8140261E-523.693245
CAATGCG2400.022.91682619
TAGGTCG3150.021.65164621
CGTCATT3300.020.0001426
ATCAACG62450.019.9047053
ATGCGAC3000.019.8007821
TCAACGC63250.019.6877294
CAACGCA63050.019.6803935
TGCGACG2700.019.55632222
GTCGTCA3400.019.41158724
CATTGCG3300.019.33565329
AACGCAG64900.019.2189226
GCTCGTC3700.019.02562339
ACGAGTC2800.018.85727326
TTAGGTC3950.018.38017320
CTAGTAC5100.018.1183573
TCGACGA1254.8614675E-717.59898637
Produced by FastQC (version 0.11.5)