FastQCFastQC Report
Thu 9 Feb 2017
SRR2131963.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2131963.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829792
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT45030.5426661139177047No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC41880.5047047934904169No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGG34330.4137181365932667No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGG32430.3908208322085534No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATTC32430.3908208322085534No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG28540.34394161428406156No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACT26710.32188789479773244No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT26370.3177904824341522No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT25060.30200339362153406No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCC24460.2947726659210983No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGA20880.25162932397516485No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGA18880.22752689830704562No Hit
ACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGC18310.22065770699163165No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG18210.21945258570822568No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGAC17110.2061962515907601No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGA17020.2051116424356947No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACGA16890.20354498476726698No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGT16860.2031834483822452No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGAT16710.20137576645713626No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA16650.20065269368709265No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTA16330.19679630558019362No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGG14280.17209131927037138No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCC14130.17028363734526242No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAAT14100.16992210096024063No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATC13730.1654631522116386No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCA13480.16245034900312366No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGT13020.15690679109945624No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACC12430.1497965755273611No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGG12160.14654274806216497No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12080.1455786510354402No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCC11940.14389148123867185No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC11570.1394325324900698No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT11320.13641972928155488No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAG11230.13533512012648952No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGT11030.1329248775596776No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGC10890.13123770776290927No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATT10850.13075565924954688No Hit
CCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC10520.1267787590143072No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAA10480.12629671050094482No Hit
GAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGG10310.12424800431915467No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGA10230.12328390729242991No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAC10180.12268134665072693No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGAT10130.12207878600902396No Hit
CATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGA9900.11930700705719023No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCG9820.11834291003046547No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACA9710.11701727661871891No Hit
ATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAAC9650.11629420384867532No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACG9610.11581215533531294No Hit
ATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAAC9480.11424549766688519No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAA9280.11183525510007326No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGG9120.10990706104662375No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACA9080.10942501253326134No Hit
TATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGC9050.10906347614823957No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC8990.10834040337819599No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAA8850.10665323358142764No Hit
TAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCA8770.10568913655470287No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8490.10231479696116616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.034.946951
GTATCAA9150.029.034321
TTAACGG450.001227023724.99735535
ATCGACC450.001227454924.9958488
TACCCTG903.2880052E-722.497625
CGTCGTA7450.021.43939210
ACCGTCG7600.021.0162478
ATACCCT754.6044024E-520.9977784
CCGTCGT7850.020.346949
TACCGTC7900.020.218167
GGTCCTA7400.020.06423613
ATACCGT8250.019.9057856
GTCGTAG8350.019.66739311
ATAATAC1051.4460438E-619.2836743
CGGTCCT7850.018.91405712
TCTGCGC600.006512983718.7480143
CGCGCTA600.0065152518.74688542
AGAGCGT600.0065152518.7468856
ATATTAA11400.018.5640931
GCAGTTA11150.018.5619415
Produced by FastQC (version 0.11.5)