FastQCFastQC Report
Thu 26 May 2016
SRR2099305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125331
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGTGAATAACACTGGACTTCTGATGTCCTGAGGTGGAAGTC9050.7220879112111129No Hit
TCTGCTGGGACACCCAAGGACACAGGAGGTTCAAAAAGCAATAGTGA4190.3343147345828247No Hit
ACTGCTAGTAGAGCTGCAGGTGGTGACCCGAGAATACACCATCAACA3810.30399502118390503No Hit
ACTGCTAGTAATGTAGCGTTCGCAGCAGCGTATACCGCAGGGTATGC3000.23936615841252365No Hit
ACTGGTAGCGCGGGCAAGGTGCAGTGTGTTCCTTCAGTTCAGATCAC2620.20904644501360398No Hit
ACTGCCCGACCTAGAGGCCGGAATTTCAGTGGCGCCTCGATGAACGA2600.20745067062418718No Hit
ACTGCTAGCCGCTGGGAATCGTACTGGCATAATGTCAACTGCTAGTT2560.2042591218453535No Hit
ACTGCTATTAGGCATCTGGGCCTGAAGATATAAGCATGGCGACAGCC2380.1898971523406021No Hit
ACTGCTAGGAGGGACAAACGATCGTACGCGGGTTACGTCAACTGTAG1970.15718377735755718No Hit
ACTGCTAGGGACCGGACTGTTCCTGCTCCTTCTACATCTGCAGATAA1900.1515985669945983No Hit
ACTGCTAGTAATGTAGCGGTTCGCAGCAGCGTATACCGCAGGGTATG1700.13564082310043007No Hit
ACTGCTAGTGAATAACAGCTGGACTTCTGATGTCCTGAGGTGGAAGT1700.13564082310043007No Hit
ACTGCTCCCTGAGGACAGCCGGGCGCGGTGGCGCACGCCTGTAGTCC1670.13324716151630484No Hit
ACTGCTAGTGACTATCTCGGCCAACGATCGTACTGACTAGCAGTTGA1650.131651387126888No Hit
ACTGCTATTAGGCATCTGGCCTGAAGATATAAGCATGGCGACAGCCA1620.12925772554276277No Hit
ACTGCTAGGTCGGATAAGTCAACTGCTAGTTTTTTTTTTTTTTTTTT1540.12287462798509546No Hit
ACTGGCAGCACAGCACCCCGCGATCCCCTCGGCCTGTCGTTCATGGA1520.12127885359567864No Hit
ACTGGTAGCGCGGGCAAGTGCAGTGTGTTCCTTCAGTTCAGATCACT1510.12048096640097022No Hit
ACTGCTAGTGAATAACATCTGGACTTCTGATGTCCTGAGGTGGAAGT1390.1109063200644693No Hit
ACTGCTAGTCGAGGGTCGGTCCGACGATCGTACAGCACGTAAGTCAA1390.1109063200644693No Hit
ACTGCTAGTCGAGGGTCGTCCGACGATCGTACAGCACGTAAGTCAAC1260.10053378653325992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA2000.041.05
CGGGTTA352.4941255E-741.029
ACACGTA256.7044566E-541.015
TCGCACC256.7044566E-541.011
TGCCCGC352.4941255E-741.09
CTCGTGT200.001108377841.036
AGTCAGC352.4941255E-741.07
CGCGGTG256.7044566E-541.024
TAGACGC200.001108377841.06
GCGAACG200.001108377841.08
GCGCGGT256.7044566E-541.023
TAGCGTG256.7044566E-541.06
TAGCGTA200.001108377841.06
TAGCGGG352.4941255E-741.06
TAGCGGA800.041.06
TAGCGAT256.7044566E-541.06
GAATCTA352.4941255E-741.08
CGACAGT1000.041.016
CGTTCGC256.7044566E-541.017
TGGTTCG200.001108377841.023
Produced by FastQC (version 0.11.5)