FastQCFastQC Report
Thu 26 May 2016
SRR2099303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119834
Sequences flagged as poor quality0
Sequence length47
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTCACCTTTCGCGCAAGCGCTGAAAGCGCAGTGTGTTGCCAATC10400.8678672163159037No Hit
CATCTGATCACTAAAACCCAGAGCGCGGTGATGGGTGGGTGTCGTCG6400.5340721331174793No Hit
GATCTGACAAAAATTATTGACGACCCAAGACGTCACGGTTTCGGTGT3680.30709147654255053No Hit
GATCTGACAGTCGGAGCTGTAAATCATGGATCTGACTTTTTTTTTTT3050.25451875093879867No Hit
GATCTGACTAAATCCACGGCAAAAGCTTCCTGGAGATCAAATGTGCA2870.2394979721948696No Hit
GATCTGACGACCCGAACGATTAGAGGCAGAGGTTAAAGCCTTGCAGG2280.19026319742310197No Hit
GATCTGACAGTCGGAGCGTAAATCATGGATCTGACTTTTTTTTTTTT2010.1677320293072083No Hit
GATCTGACCAAAAAAGAGGTGCGGACCAGGGGAATCCGACTGTTTAA1960.16355959076722798No Hit
GATCTGACAGAGCGAGGATGACCGTCCATGGATCTGACTTTTTTTTT1590.13268354557137374No Hit
GATCTGACACGCTATCTGGCTCAGTTCTACATTTGTGTTGCACGTCA1370.11432481599546039No Hit
GATCTGACGGATATATAAGTTGAACGCACACATCGCAGAGCAGTTTC1310.109317889747484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAC553.6379788E-1241.00000411
GGTCGGA750.041.0000041
CTGACCG3550.041.0000044
TCGGAGC750.041.00000411
GTCGGAG750.041.00000410
AGCCGGC200.001108182541.017
ACACGAC505.820766E-1141.07
CGTGCGC256.7026864E-541.041
GACGTTT352.4928886E-741.06
TTTCGCG1400.041.010
TAGGTGA200.001108182541.016
GACGTGT200.001108182541.06
GACGTAT800.041.06
GACCGAA505.820766E-1141.06
TGAACGC256.7026864E-541.021
CGTCTCA256.7026864E-541.016
TGACGTT1050.041.05
TGACGTG650.041.05
TGACGTA1650.041.05
TGACGGT1700.041.05
Produced by FastQC (version 0.11.5)