FastQCFastQC Report
Thu 26 May 2016
SRR2099301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163589
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACTGAATAAAAAAACGACTCACTATAGTGAGTCGTATTACCGGC10170.6216799418053781No Hit
TGCACTGAATAAAAAAACGGCCGACGATCAGTCATAAAAAAAATCAG4210.25735226696171504No Hit
TGCACTGAATCTGTAGTCTGATTTTTTTTTTTTTTTTTTTTTTTTTT3720.22739915275476955No Hit
TGCACTGACCGTCCACAGTGCAAGCTTGTGGACGGGACTGATCGTCG3500.2139508157638961No Hit
GACACTGACAGTCTGGTGATCGGTCGCTGGGTGGAAGCGGGCGAAGC3360.20539278313334028No Hit
TGCACTGAAAAAGAATGGTGGGCTGTTGGTACGTAATGGGCGACAAC3300.20172505486310202No Hit
TGCACTGAGCCCCACGAGAAAGAGAAGGCAGATTCTCTGGCCAAGGA3280.20050247877302263No Hit
TGCAGCGAATTGGCGCCTGATGCCCTGGTGGAGCCGCCTGGGCAAGA3170.1937783102775859No Hit
TGCAATGCAACGAAAGTAATAATCTCCATCACTCGTTCACTGGAGAA2560.156489739530164No Hit
TGCACTGAAGGGAAGATATTGAGTACTACCTTCAGTTACTTGCCGAA2460.15037685907976697No Hit
GACACTGACAGTCTGGTGGATCGGTCGCTGGGTGGAAGCGGGCGAAG2440.14915428298968755No Hit
TGCACTGAATAAAAAAACCGGCCGACGATCAGTCATAAAAAAAATCA2370.14487526667440964No Hit
TGTACTGACATTAGGCGACAAACACTGCCTGTACGTAGCTTATAGCA2230.1363172340438538No Hit
TGCACTGAACTGTTGCGCAGTCAAAGCCTCAAGCTTGCACTGATTGT2110.12898177750337736No Hit
TGCACTGACCGTCCACACTCTGAACCCCTATAGTGAGTCGTATTAAC1870.11431086442242448No Hit
TGCACTGAAAAGACCCGCTTGTGCTTCAACCAATCTAAATTGGATTC1720.10514154374682895No Hit
TGCACTGAATAAAAAAAAGTGAGTCGTATTACCGGCCGACGATCAGT1700.10391896765674954No Hit
TGCACTGACCCGTATGCGGAACTAGGAGGGCTGTGATGGGTGTGCCC1690.10330767961170984No Hit
TGCACTGAAAAAGAATGGGTGGGCTGTTGGTACGTAATGGGCGACAA1670.10208510352163042No Hit
TGCACTGAATTTGGAAATCGTCCTTGTCCGGGGAGGAGCTCGTCACC1660.10147381547659072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTG200.001106848941.01896710
GATACGT352.491306E-741.0189677
ACACGGC200.001106848941.0189678
GAGTCGA401.5306796E-841.0189677
ACACCCG200.001106848941.01896711
GACCGTC3950.041.0189677
GAGTAGC352.491306E-741.0189677
GAGTACG304.0733594E-641.0189677
GGCGTAG200.001106848941.01896710
CGCTGAG650.041.0189673
CTGACCG5300.041.0189675
ACTTAAC200.001106848941.0189678
CATTAGG553.6379788E-1241.0189679
ATCCGGT352.491306E-741.0189678
GGCTTAT200.001106848941.0189679
ACGATGT200.001106848941.0189679
CCCGTCC200.001106848941.01896710
GAACGTA900.041.0189677
TCCGTGC401.5306796E-841.0189679
GACATTA600.041.0189677
Produced by FastQC (version 0.11.5)