FastQCFastQC Report
Thu 26 May 2016
SRR2099300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843741
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGACTGACAGGAGCCAGATCGTCAGGAAAAGGAGTACCTAGACTG48710.5773098616755616No Hit
CTAGACTGTTTACAGACGTCCGACGATCGTCGGACTGTAGAACTCTG40320.4778717639654823Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGGCCTCCTCAGTCGGACTGTAGAACTCTGAACCCCTATAG28480.3375443412137137Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTATAGTGGATAATGGAGCCGGTAGAACTCTGAACCCCTATAGTGAG26320.3119440681441343No Hit
CTATAGTGAGATTATGAGTCCGACGATCTTCGGACTGTAGAACTCTG26320.3119440681441343Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTAGACTGCGAGGACGAGATCGGGGCGTCGAACACGCGCTTGGTCGT24920.2953512985619995No Hit
CTATAGTGTCACTCTAAAGTGAAGCTGATCGTCGGACTGTAGAACTC17160.20337994716388086Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTAGACTGTACCCAGTTGCAGTGGTGGTAGTAGGAAGATGTCGGGCG16820.1993502745510767No Hit
CTAGACTGTAAATCAATGTCATAGGCAAGTGATGATTGTGTTTTCTC15120.17920191148705586No Hit
CTAGACTGCGAGGACGAGGATCGGGGCGTCGAACACGCGCTTGGTCG14960.1773055949633833No Hit
CTATAGTGTCACTCTAAGTCGGACTGTAGAACTCTGAACCCCTATAG10300.12207537621142034Illumina DpnII expression Adapter 1 (95% over 23bp)
CTAGACCGCAGAACAGCGGAACAATGCAGGCGCAAGACGCACAAGGC9500.11259379359305759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAT200.001111668141.002499
TGTAACG1350.041.002497
TTTCCGC200.001114264640.9830511
ACTCGCG304.118352E-640.9830510
CACTCTA24700.040.81712710
TGCGAGG8500.040.76137
GACTGCG45650.040.7330324
AGACTGC162650.040.6369633
AGACTGG176850.040.5619773
AGACTGA202600.040.4560623
ACTGCGA20550.040.3041535
GACTGCA45700.040.2398644
ACTCTAA25150.040.08680311
CAGTTGC2200.040.06586513
GACTGAA65600.039.9711764
GACTGGT34700.039.879944
GACTGAG53400.039.8507354
ACTGCCG11100.039.709625
GACTGGG61100.039.6603474
ATAGTGG30250.039.6470383
Produced by FastQC (version 0.11.5)