FastQCFastQC Report
Thu 26 May 2016
SRR2099299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158571
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGACTGACAGGAGCCAGATCGTCAGGAAAAGGAGTACCTAGACTG14560.9182006798216572No Hit
CTAGACTGTTTACAGACGTCCGACGATCGTCGGACTGTAGAACTCTG9690.6110827326560342Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGGCCTCCTCAGTCGGACTGTAGAACTCTGAACCCCTATAG9170.5782898512338321Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTATAGTGAGATTATGAGTCCGACGATCTTCGGACTGTAGAACTCTG8390.5291005291005291Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTAGACTGCGAGGACGAGATCGGGGCGTCGAACACGCGCTTGGTCGT8390.5291005291005291No Hit
CTATAGTGGATAATGGAGCCGGTAGAACTCTGAACCCCTATAGTGAG8030.5063977650390047No Hit
CTAGACTGTCCGTCTGACGACTCACTATAGTGAGTCGTATTACCGGC7000.44144263452964283No Hit
CTAGACTGTACCCAGTTGCAGTGGTGGTAGTAGGAAGATGTCGGGCG5690.3588297986390954No Hit
CTAGACTGCGAGGACGAGGATCGGGGCGTCGAACACGCGCTTGGTCG4880.3077485795006653No Hit
CTAGACTGCCAGTATTCCTCACTATAGTGAGTCGTATTACCGGCCGA4770.3008116238151995No Hit
CTAGACTGTAAATCAATGTCATAGGCAAGTGATGATTGTGTTTTCTC4430.2793701244237597No Hit
CTATAGTGTCACTCTAAGTCGGACTGTAGAACTCTGAACCCCTATAG3150.1986491855383393Illumina DpnII expression Adapter 1 (95% over 23bp)
CTAGACCGCAGAACAGCGGAACAATGCAGGCGCAAGACGCACAAGGC3020.19045096518278878No Hit
CTATAGTGTCACTCTAACTCTGAACCCCTATAGTGAGTCGTATTACG2680.16900946579134898No Hit
CTAGACTGACGTGAAGTGTCCGACGATCGTCAAAATACGAAGTACCT2550.16081124543579847No Hit
CTATAGTGTCACTCTAAAGTGAAGCTGATCGTCGGACTGTAGAACTC2510.15828871609562908Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTAGACTGAAAATGAATAATTAGCTGGGTGTGGTCGCGGGTGCCTGT2310.14567606939478214No Hit
CTAGACTGCACTGTGCGCTCAATCTTTAATCAAGCAGGGACAAATCC2160.13621658436914694No Hit
CTAGACTGGAGAGACCTCGTCCGACGATCGTCATTTGTGGAGGTACC2120.13369405502897755No Hit
CTAGACTGGATTAGTTGGTTTCAGGTTAAATAAACCTTTTGCGTCGG2090.1318021580238505No Hit
CTAGACCGGTTAACACGGGTGAACTTGTGCTCCTTAGCCTTATCAGC2090.1318021580238505No Hit
CTAGACTGATATAATTCGGGTGAGAGTAGTAGCGCTTTACTGAAAAA2020.12738773167855408No Hit
CTAGACTGGACATGAAGAAACAAGGTCCCCATCAAATGACCATACTT1960.12360393766830001No Hit
CTAGACTGAACGGTAATGGTACATTACCGTTTGACGATCGTCGGACT1940.12234267299821532No Hit
CTAGACTGTGAGGTGCGAAAGGACCCAAGGAGCTGAAGGGATTTGCA1920.12108140832813061No Hit
CTAGACTGCCTTCCCATTGCAGCGCCAGCGGCGCGGCGGTGGAGATG1870.11792824665291887No Hit
CTAGACTGCCGCCTCCTCATCAACCTGGAAATCCCGGAAAAGCGCGT1830.1154057173127495No Hit
CTATAGTGGCCTCCTCAAGTCGGACTGTAGAACTCTGAACCCCTATA1740.10973002629736837Illumina NlaIII expression Adapter 1 (96% over 26bp)
CGACACTGACAACAACCGTTCGCCACGGCTCGGTGGGGGAGAAGGCC1720.10846876162728368No Hit
CTAGACTTAGTCTGGAAAGTCTAGGTACTTCCAGACTTGACGATCGT1640.10342370294694489No Hit
CTAGACTGCGTGTCAGTGGGAAACTTTGCCAGGGCACCAAGGACTTG1640.10342370294694489No Hit
CTATAGTGCTATTCTGAGTCGGACTGTAGAACTCTGAACCCCTATAG1640.10342370294694489Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTATAGTGGCCTCCTCAGGTCGGACTGTAGAACTCTGAACCCCTATA1630.10279307061190254Illumina NlaIII expression Adapter 1 (96% over 26bp)
CTATAGTGTCACTCTAATAGTGAGTCGTATTACGGCCTCACTATAGG1610.10153180594181786No Hit
CTATAGTGTCACTCTAAGCCTCACTATAGGACTTTAGAGTGAAGCTG1590.10027054127173317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAC650.041.01072716
AGATCGG650.041.01072717
CTCGTCC256.702186E-541.01072716
TTAATCG256.702186E-541.01072712
ATAGGGT304.0782033E-641.01072714
TAAGTCG650.041.01072715
CGTTCGC256.702186E-541.01072717
TAACCGA256.702186E-541.01072715
TCTAACG256.702186E-541.01072713
AGGATCG553.6379788E-1241.01072717
AACACGG256.702186E-541.01072712
CAGCGGA352.4945984E-741.01072314
AACCGGT200.001107842141.01072314
TCGCATC200.001107842141.01072312
CGAACCT200.001107842141.01072314
GTCGAGA200.001107842141.01072315
CGCATAC200.001107842141.01072313
CAACCGG200.001107842141.01072313
AAGGTAA200.001107842141.01072316
TCCTAGA459.458745E-1041.01072317
Produced by FastQC (version 0.11.5)