FastQCFastQC Report
Thu 26 May 2016
SRR2099298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402898
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTAAAGAGAAACGACTCACTATAGTGAGTCGTATTACCGGC37050.9195875879254798No Hit
GACTGCGTGACTACTACGCAACTTCGTCAGCCGCACCGAGGACCTGT37020.9188429825911273No Hit
GACTACGTTATGCCGCGACAGACTTATCAGTGACTACGTTTTTTTTT20430.507076232694131No Hit
GACTACGTGGTTCGTGCGGTAGGATGGTGGGGACATCCTCTTGGAGA18520.4596696930736812No Hit
GACTACGTGGTTCGTGCGGGTAGGATGGTGGGGACATCCTCTTGGAG14940.37081345650760245No Hit
GACTTCCTTGATGGCTGGGGCAGGCGATCAGAATGGCTTTGAACAGA8520.21146791495614276No Hit
GACTACGTGACTTCTAGGGTGGGGTGGGGGGAGGGGGGACTGATAGC8270.20526287050320427No Hit
GACTACGTAACCCGCCCATCTCCTCTAAAATTTTCAATCCAGTTATT5440.1350217672959409No Hit
GACTACGTTTATGGGTTGTCCGACGATCTCGAAAATGATAACAGTGA5390.13378075840535322No Hit
GACTACGTGGACTAAAGGATGCAGCTTTCTCCTCCATCTGGTCTCCC5140.12757571395241477No Hit
GACTACGTTGAGGTGAACAGTCCGACGAATCTCGAATCAAGAAACAG5090.12633470506182706No Hit
GACTTCCTTGATGGCTGGGGGCAGGCGATCAGAATGGCTTTGAACAG5040.1250936961712394No Hit
GACTACGTTAGGCGGATCTTTCTTCCTGGTAGAGCATTGCCACGATG4870.12087426594324122No Hit
GACGACGTAATGAAACTCGCCAGACGTTTGCTTTAGATCAAGAGAAG4680.116158432159008No Hit
GACTACGTGACTTCTAGGGGTGGGGTGGGGGGAGGGGGGACTGATAG4570.11342821259971506No Hit
GACTACGTTTGTTGTGATTCCTGCCGAGGCAGGTCCTGGCGGAAGGG4520.11218720370912737No Hit
GATTACGCATCCAAACACGGAAATTGCAATTTCCGTTCTAAGTGCAA4510.11193900193100983No Hit
GACTACGTCTTGGTCATCGACTCACTATAGTGAGTCGTATTACCGGC4510.11193900193100983No Hit
GACTACGTTAATAGAGGAGTCAGAGACGCCGCGAGGTCGCCGCCACC4430.10995338770606954No Hit
GACTACGTTATGCCGCGAGACAGACTTATCAGTGACTACGTTTTTTT4390.10896058059359937No Hit
GACTACGTGGTTCGTGCAGGTAGGATGGTGGGGACATCCTCTTGGAG4280.10623036103430644No Hit
GACTACGTCGATTGGTTTCCGACGATCTCGAGCCGTTTCTCAGTGAC4160.10325193969689599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG304.0939685E-641.0106858
AGCACGC304.0939685E-641.0106859
CCTTAGT459.531504E-1041.010689
ACGTTAC3850.041.010685
TAACGCT650.041.010689
CGTTCCG950.041.010686
CTAACGG200.001109884741.010689
GTTCGAT459.531504E-1041.010687
TGCGACT200.001109884741.010688
GTACTAG1100.041.010687
TTAACGC650.041.010688
GTCCCGT401.5430487E-841.010687
GTTATCC401.5430487E-841.010687
AATCGCG200.001109884741.010689
ACACGCG401.549779E-840.9903215
GCGCGTT304.107971E-640.9903214
AACGCTG650.040.9903210
GCTCGCC200.001112600140.9903213
CTAGGGG553.6379788E-1240.9903214
TGTCGCG200.001112600140.9903210
Produced by FastQC (version 0.11.5)