FastQCFastQC Report
Thu 26 May 2016
SRR2099297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84248
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTGCGTGACTACTACGCAACTTCGTCAGCCGCACCGAGGACCTGT12241.4528534802013104No Hit
GACTACGTAAAGAGAAACGACTCACTATAGTGAGTCGTATTACCGGC12191.4469186212135599No Hit
GACTACGTTATGCCGCGACAGACTTATCAGTGACTACGTTTTTTTTT6790.8059538505365113No Hit
GACTACGTGGTTCGTGCGGTAGGATGGTGGGGACATCCTCTTGGAGA6270.7442313170639066No Hit
GACTACGTGGTTCGTGCGGGTAGGATGGTGGGGACATCCTCTTGGAG5050.5994207577627956No Hit
GACTACGTCTTGGTCATCGACTCACTATAGTGAGTCGTATTACCGGC4480.5317633653024404No Hit
GACTTCCTTGATGGCTGGGGCAGGCGATCAGAATGGCTTTGAACAGA2820.33472604690912544No Hit
GACTACGTGACTTCTAGGGTGGGGTGGGGGGAGGGGGGACTGATAGC2570.3050517519703732No Hit
GACTACGTTTATGGGTTGTCCGACGATCTCGAAAATGATAACAGTGA1860.2207767543443168No Hit
GACTACGTGGTTCGTGCAGGTAGGATGGTGGGGACATCCTCTTGGAG1720.20415914917861552No Hit
GACTACGTTGAGGTGAACAGTCCGACGAATCTCGAATCAAGAAACAG1680.19941126198841516No Hit
GACTACGTGACTTCTAGGGGTGGGGTGGGGGGAGGGGGGACTGATAG1560.18516760041781408No Hit
GATTACGCATCCAAACACGGAAATTGCAATTTCCGTTCTAAGTGCAA1540.1827936568227139No Hit
GACTACGTAACCCGCCCATCTCCTCTAAAATTTTCAATCCAGTTATT1510.17923274143006362No Hit
GACTACGTTATGCCGCGAGACAGACTTATCAGTGACTACGTTTTTTT1510.17923274143006362No Hit
GACGACGTAATGAAACTCGCCAGACGTTTGCTTTAGATCAAGAGAAG1510.17923274143006362No Hit
GACTTCCTTGATGGCTGGGGGCAGGCGATCAGAATGGCTTTGAACAG1430.1697369670496629No Hit
GACTACGTTAATAGAGGAGTCAGAGACGCCGCGAGGTCGCCGCCACC1420.1685499952521128No Hit
GACTACGTTAGGCGGATCTTTCTTCCTGGTAGAGCATTGCCACGATG1340.1590542208717121No Hit
GACTACGTGGTTAAGCTGTCCGACGATCTCGAAAAAGCAAAACAGTG1340.1590542208717121No Hit
GACTACGTGGACTAAAGGATGCAGCTTTCTCCTCCATCTGGTCTCCC1290.15311936188396164No Hit
GGCCACGTTAACTCGCGGCTGCCGGTGACGGTGCTGACACTCGTCGT1190.14124964390846073No Hit
GACTACGTGGTTCGTGCTGGTAGGATGGTGGGGACATCCTCTTGGAG1170.13887570031336055No Hit
GACTACGTTCCCAGCGGGGTACCACCATTGGACCTATCTTGGCTTCT1160.13768872851581046No Hit
GACTACGTTATGCCGCGTACAGACTTATCAGTGACTACGTTTTTTTT1140.1353147849207103No Hit
GACTACGTTATGCCGCGGACAGACTTATCAGTGACTACGTTTTTTTT1080.12819295413540974No Hit
GACTACGTCAGCTCACACGCGGCGTCGCCAGGAGGAGCGCGCGGGCA1070.12700598233785965No Hit
GACTACGTGATTCGTATGAACATGCCAAGATTGTCGTCTTGTTGAGT1010.11988415155255912No Hit
GACTACGTTTACTTCAACAGAAGCACCCAGGAGTATCTTCTCACCTT980.11632323615990885No Hit
GACTACGTTATGCCGCGCACAGACTTATCAGTGACTACGTTTTTTTT870.10326654638685784No Hit
GACTACGTTGCTGCAATAGATAAGGCCAAGGATGGCCTTATCTGAAA870.10326654638685784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAG200.001103387141.02198440
TAAGTGC200.001103387141.02198439
GTTGACG256.6879504E-540.9976277
GTGTTAC256.6879504E-540.9976277
CGTGGTT2000.040.9976276
TAATATG256.6879504E-540.9976278
CATGGAC256.6879504E-540.99762710
CGACGTG600.040.9976273
ATGGCTG1000.040.99762711
CCGATGT256.6879504E-540.99762711
ACGTTAC600.040.9976275
CGTGCAC256.6879504E-540.9976276
GAGTCGG256.6879504E-540.99762713
ACGCAGA304.064301E-640.9976275
AACCCGC1100.040.9976279
GTCCTTA256.6879504E-540.9976277
GACGTGT600.040.9976274
ATGGACC256.6879504E-540.99762711
CGTGAGC304.064301E-640.9976276
CTATACG256.6879504E-540.9976279
Produced by FastQC (version 0.11.5)