FastQCFastQC Report
Thu 26 May 2016
SRR2099296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565356
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGATCGGCATAGGCGCCAGCGGCCGGCACTACCTCGGACCGGGA14690.25983628014914495No Hit
TGACGGGCGGGTAGTTCGGCGACGGCTTATTTGCCTCGCGATGCAAA12150.2149088362023221No Hit
TGACGATCCAGAGGGCCTGAGGCCCTCTGCATGGATCGTCGGACTGT11200.19810526464740802No Hit
TGACGATCATATCAAAACGACTCACTATAGTGAGTCGTATTACCGGC10160.17970977578729155No Hit
TGACGATCCACTAGCAAGTGTCCCCCCTGCGCCGTGTGAACCAGGCC8280.1464563920786195No Hit
TGACGATCATATCAAAAGCGACTCACTATAGTGAGTCGTATTACCGG8090.14309567776763668No Hit
TGACGAACGAACACATTGCTCAGTTGGGCCACGATATCTCAATGGAG7980.1411500010612782No Hit
TGACGATCGAAGAGAACATTGAGTGCCAGCAGATACAAAACAAGATA7740.13690488824740518No Hit
TGATGCTCGAAGACCGGGGACGAACGACGAGCCGAAAAAAAGGCTGC7630.1349592115410467No Hit
TGACGATCAGAACATGAGTGAGGATCCTTATCCCATTCATCTTGCAG7470.132129136331798No Hit
TGACGATCCAAATGGTTGCTGTGTGACAGAACCACACTGTCCCTCCT7050.12470018890752022No Hit
TGACGATCGCCGCGTACTTCCTGTACCCAGTGTTACCCTTGTTATTT6030.10665845944855985No Hit
TGGAGATCCTGCGCAATGGCGCCTACAAGAAGGTGCAGGCGAAGTAT5900.1043590233410453No Hit
TGACGATCGTACCCGGAGGCAGAGGTTGCAGTGAGCCGAGATAGTGC5810.1027671060358429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC600.041.007177
CCGGACC200.001110732941.007178
CGACTCG401.5459591E-841.007174
TCGGTCG800.041.007177
TCTGCGC553.6379788E-1241.0071687
CACGAAT1100.041.0071688
GCGATGT1050.041.0071683
CCGTTAG256.727553E-541.0071688
TACGTTT200.001112184540.99628415
CGCCGTT256.7451256E-540.9890310
CGATCGG35250.040.541844
CGATCAA36900.040.4515154
ACGATCG133300.040.222713
ACGTTCA2000.039.9819873
ACGATCA132450.039.969993
CGATCCG29500.039.964614
CGATCAC34200.039.928034
GATCCGT5300.039.846595
CGATCGA40600.039.8456344
ATCGATT4550.039.655286
Produced by FastQC (version 0.11.5)