FastQCFastQC Report
Thu 26 May 2016
SRR2099295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107879
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGATCGGCATAGGCGCCAGCGGCCGGCACTACCTCGGACCGGGA4390.40693740208937795No Hit
TGACGGGCGGGTAGTTCGGCGACGGCTTATTTGCCTCGCGATGCAAA4180.38747114823088835No Hit
TGACGATCATATCAAAACGACTCACTATAGTGAGTCGTATTACCGGC3600.3337072090026789No Hit
TGATGCTCGAAGACCGGGGACGAACGACGAGCCGAAAAAAAGGCTGC3480.32258363536925627No Hit
TGACGATCATATCAAAAGCGACTCACTATAGTGAGTCGTATTACCGG3030.28087023424392144No Hit
TGACGATCCACTAGCAAGTGTCCCCCCTGCGCCGTGTGAACCAGGCC2800.25955005144652804No Hit
TGACGAACGAACACATTGCTCAGTTGGGCCACGATATCTCAATGGAG2790.25862308697707614No Hit
TGACGATCGAAGAGAACATTGAGTGCCAGCAGATACAAAACAAGATA2530.23452201077132714No Hit
TGACGATCAGAACATGAGTGAGGATCCTTATCCCATTCATCTTGCAG2200.2039321832794149No Hit
TGGAGATCCTGCGCAATGGCGCCTACAAGAAGGTGCAGGCGAAGTAT1950.18075807154311774No Hit
TGACGATCGTACCCGGAGGCAGAGGTTGCAGTGAGCCGAGATAGTGC1860.17241539131805078No Hit
TGACGATCCCATATATGGGGGCGGAAAGCCAAACGCGGAGGCGCGTG1840.17056146237914702No Hit
TGACGATCCCGGGAACCGTTCGGCAGCCTTGCTGATTGTGAATGTAG1650.15294913745956118No Hit
TGACGATCCAAATGGTTGCTGTGTGACAGAACCACACTGTCCCTCCT1580.146460386173398No Hit
CGATGATCGATGCGGCAGTGATGTCGGGGTCTACCGGTTTGCGTTGC1460.13533681253997534No Hit
TGACGATCGCCGCGTACTTCCTGTACCCAGTGTTACCCTTGTTATTT1430.13255591913161968No Hit
TGACGATCATATCAAAACGCGACTCACTATAGTGAGTCGTATTACCG1380.12792109678436026No Hit
TGACGATCAAGGGTCAAATTGGATTCAAAGGTAATCGTTGCCGGAAC1350.1251402033760046No Hit
TGACGATCCAGAGGGCCTGAGGCCCTCTGCATGGATCGTCGGACTGT1350.1251402033760046No Hit
TGACGATCACTGGGCACGTCCGACGATCCATGGCAGGTAGATCAGTG1260.11679752315093762No Hit
TGACGATCCCGGGAACCGGTTCGGCAGCCTTGCTGATTGTGAATGTA1230.11401662974258196No Hit
GGACGATCCAGAGGGCCTGAGGCCCTCTGCATGGATCGTCGGACTGT1210.1121627008036782No Hit
TGACGATCGGATAAGATGTCCGACGATCCATGAGCGGTTGGTCAGTA1210.1121627008036782No Hit
TGATGAACTGCATATTGGTGACCGATAACGCCCAGAGCGTCTACGAG1200.11123573633422632No Hit
TGACGATCGCAGCCAGTTTCGTTTTCGTCCGTCAGCAAGCGGGTCAC1150.10660091398696687No Hit
TGACGATCGCATAAGGGCATGGAAATGTAGTCAGTGACGATCTTTTT1120.10382002057861123No Hit
TGACGATCAGAGCGTGAAGGGCAAAGACCCCAATGCCCCCAAGAGGC1080.10011216270080368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCG200.001101411641.0473517
AAGTGAC200.001101411641.0473517
CGCCAGC401.5130354E-841.0473517
GATGTCG200.001103931541.02830520
AAAAGCG600.041.00927714
AAAACGC256.689273E-541.00927714
CGGGGAC256.689273E-541.00927715
GAACCGG256.689273E-541.00927713
ATACAGC256.689273E-541.00927713
CCGGGGA200.001106455941.00927414
ACCGGGG352.4853762E-741.00927413
GCGTTAG200.001106455941.00927414
GTTACAC200.001106455941.00927414
TTACACA200.001106455941.00927415
ACAAGAC200.001106455941.00927414
TGCGTTA200.001106455941.00927413
ATCCTTC304.0800187E-640.9902656
CTATGGG553.6379788E-1240.9902658
TCGCATA553.6379788E-1240.9902657
AGCCGAG304.0800187E-640.99026534
Produced by FastQC (version 0.11.5)