FastQCFastQC Report
Thu 26 May 2016
SRR2099294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182881
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGCAAGCAAGCGACGCCGACGGCTTTTAGCATTGCTTCTTTTT58350.4932871523001891No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC200.001111420541.00570713
GATGCAC228100.040.6607063
TGCACGA20950.040.612535
ATGCAAC85500.040.5243954
GATGCAA403550.040.402783
GATGCAG252600.040.2717933
ATGCAAG106000.040.1722954
ATGCACG60050.040.1162954
TGCAACC18750.040.0198825
TGCACCG12600.039.702265
TGCATAC21850.039.5965165
ATGCACC49000.039.581394
ATGCAGG65650.039.4425134
ATGCATA72850.039.3716964
TGCACGC12300.039.3371475
ATGCACT55350.039.300114
CCGCGCA4550.039.18171711
ATGCAAT72700.039.1427234
ATGCATG52850.039.1419224
ATGCACA72200.039.129834
Produced by FastQC (version 0.11.5)