FastQCFastQC Report
Thu 26 May 2016
SRR2099293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293506
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGCAAGCAAGCGACGCCGACGGCTTTTAGCATTGCTTCTTTTT17880.6091868650044633No Hit
CTGATGCACGACGCTTAGCGACGGACCCTTGCCACCGCAGCGGGGGT3360.11447806859144277No Hit
CTGATGCACTCCCGAATATGGAAGGCCCACAGTCACCTGGTCAGCTC3060.1042568124672068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG200.001108363741.0184112
TTAGGCG200.001108363741.0184115
TCGTTCC200.001108363741.0184112
CCGTCCT200.001108363741.0184116
TTGACGG200.001108363741.0184113
TGAATCG256.708771E-541.0184112
TGACGCA459.549694E-1040.9974442
CATCGTT200.001111155740.9974447
CATCGCA256.729034E-540.9974447
GTGCGAC600.040.9974444
GCACCGG650.040.997446
CATTTCG352.5106965E-740.997447
TCGTTAT200.001112087640.9904610
GACGTAC256.735799E-540.9904611
TCTACGC200.001112087640.9904611
TGACACG200.001112087640.990469
GTATCGG200.001112087640.990469
CCGCGCA1200.040.9904611
CGTCCGC200.001112087640.990469
CCGCCTA304.104055E-640.9904625
Produced by FastQC (version 0.11.5)