FastQCFastQC Report
Thu 26 May 2016
SRR2099290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268669
Sequences flagged as poor quality0
Sequence length42
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTGTGTTCGCGGCCGCGGCATGCAGTTCTCCGACCCGG16380.6096721244356439No Hit
CTACGAGTTATCGATGAGGGCAAAAGCTGAACCTTTAGTCAG14460.5382087252343962No Hit
CTACTAATATCAGGTTAATGAGTGAAAAAGCTACTTTGTTTG14250.5303924159467598No Hit
CTACGAGTCTTTGGCAGAGGAGCACAACATTGTGTTCATGCC11530.4291526004116589No Hit
CTACGAGTCCGGGCTACGAGTTTTTTTTTTTTTTTTTTTTTT7110.26463790016712013No Hit
CTACGAGTCCCTATTTTAAATGGCTGCACAATAGCCTGCCAT5440.20247963107020164No Hit
CTACGGGTTGGCGCGCAGCGTCTCGCAAGGACGCGCGGCGGG5370.19987419464098946No Hit
CTACGAGTAGGGGCTCATATACACAGTTACGCTAACTCAGGT4860.18089172922815805No Hit
CTACGAGTGCCTCATCAGCCCAGTTCTAGATTTCCTGATAAC4680.1741920355530411No Hit
CTACTATTCTGTTAACCTTGTGGGCATGTTTTGTGCAACTGA4270.15893162218194135No Hit
CAACCAGTCAGGGGTTAGCAAAAATCTCTGACATTTCATCCA4000.14888208166926592No Hit
CTACGAGTGGTTAGTGAGAGCATGGCACAAACTAGGGCACTT3720.1384603359524173No Hit
CTACGAGTCTGGTTCCCTTGCCACTGCCTAGCCCATTGCGGG3260.1213388965604517No Hit
CTACGAGTTTTAGGGCTCCCCCAGCAGCCCTGACCCACCTGG3250.12096669135627854No Hit
CTACGAGTTATTAAAAGAGAAGAATTGTAATATTCCCAACCC2990.11128935604777625No Hit
CTACGAGTTTTAGGCTCCCCCAGCAGCCCTGACCCACCTGGC2960.11017274043525678No Hit
CTACGAGTAGGGTCTCATATACACAGTTACGCTAACTCAGGT2870.10682289359769828No Hit
CTGCGCGTGAGGTTCCAGCGCGGTGGACAGCGGTGCACCGCC2790.10384525196431296No Hit
CTACGAGTTATTGCTGGAGATATTATAATAAATGATGGCAAA2730.10161202073927399No Hit
CCACGAGTAGTCGTGAGGCAGGCGAGCTCACCTGAAGACTGC2710.10086761033092766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTA200.002065509636.13647535
AGTCACG650.036.0354236
CGAGTCT13250.036.0354234
GTATGCG551.6370905E-1136.0354237
TCGCTTG356.8989175E-736.0354238
CGAGGCG551.6370905E-1136.0354234
CGAATAG1250.036.0354234
ACTCGAT356.8989175E-736.0354236
CGCGTAG700.036.0354234
GTGCGCC453.3705874E-936.0354237
AGTGTCG1250.036.0354236
GTCGGTA356.8989175E-736.0354237
GTTGCGG356.8989175E-736.0354237
ACGAATG650.036.0354233
ACGAATA2200.036.0354233
TCCGGCG200.002094104236.035428
TGTCACT251.4380884E-436.035428
AGCGTAG200.002094104236.035424
TTGGCGC1050.036.035428
GCGACTT251.4380884E-436.035429
Produced by FastQC (version 0.11.5)