FastQCFastQC Report
Thu 26 May 2016
SRR2099289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68261
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTGTGTTCGCGGCCGCGGCATGCAGTTCTCCGACCCGG5190.7603170185025124No Hit
CTACTAATATCAGGTTAATGAGTGAAAAAGCTACTTTGTTTG4490.6577694437526552No Hit
CTACGAGTCTTTGGCAGAGGAGCACAACATTGTGTTCATGCC3280.48050863597075927No Hit
CTACGAGTTATCGATGAGGGCAAAAGCTGAACCTTTAGTCAG3170.464394017081496No Hit
CTACGAGTCCGGGCTACGAGTTTTTTTTTTTTTTTTTTTTTT2660.3896807840494572No Hit
CTACGAGTCCCTATTTTAAATGGCTGCACAATAGCCTGCCAT1770.25929886758178167No Hit
CTACGGGTTGGCGCGCAGCGTCTCGCAAGGACGCGCGGCGGG1740.2549039715210735No Hit
CTACGAGTGCCTCATCAGCCCAGTTCTAGATTTCCTGATAAC1710.2505090754603654No Hit
CTACGAGTAGGGGCTCATATACACAGTTACGCTAACTCAGGT1550.22706962980325512No Hit
CTACTATTCTGTTAACCTTGTGGGCATGTTTTGTGCAACTGA1420.2080250802068531No Hit
CAACCAGTCAGGGGTTAGCAAAAATCTCTGACATTTCATCCA1290.18898053061045106No Hit
CTACGAGTGGTTAGTGAGAGCATGGCACAAACTAGGGCACTT1240.18165570384260413No Hit
CTACGAGTTATTAAAAGAGAAGAATTGTAATATTCCCAACCC1020.14942646606407758No Hit
CTGCGCGTGAGGTTCCAGCGCGGTGGACAGCGGTGCACCGCC1020.14942646606407758No Hit
CTACGAGTTATTGCTGGAGATATTATAATAAATGATGGCAAA970.14210163929623063No Hit
CTACGAATAGTTGGAAGGGAAGAGGGGATGGGATAGGGGGTT950.13917170858909186No Hit
CTACGAGTGGGAGGGAATTATCTTCTCCTGCTGTCTGGTGAA930.13624177788195307No Hit
CTACGAGTCTGGTTCCCTTGCCACTGCCTAGCCCATTGCGGG920.1347768125283837No Hit
CTACGAGTAGGGTCTCATATACACAGTTACGCTAACTCAGGT900.13184688182124493No Hit
CTACGAGTGGCACAAGACCAGAATCCAGCTCTGTGAGGGGTG890.13038191646767555No Hit
CTGCTAGTACAAGCTCGCACGAGCTAGTCTTTTTTTTTTTTT870.12745198576053676No Hit
CTTCGAGCACGGGCCGGACGGGGTGCTGGAGCCGGTCGCACC870.12745198576053676No Hit
CTACGAGTGCGACTTACAACAGCCAAAGACTGTTAGGATGAC860.12598702040696735No Hit
CTACGAGTCTTTTGGTGAAAGACTGAAACGCTTTTCCCCTAA850.124522055053398No Hit
CTACGAGTTGTTGGGCAGTGTCTGTGCCATGCCTAATAGATA820.12012715899268982No Hit
CTACGAGTGCCGGGCAGGTTGGTGAGCCTTGCGGATTCTTCA750.1098724015177041No Hit
CTAAAAGTACAGAGTATCTAAGCGTAAAATAGTTCGCTTAGA740.10840743616413473No Hit
CCACGTGTGTTCGGCGGCCGCGGCATGCAGTTCTCCGACCCG720.10547750545699594No Hit
CTACAGGTCATGCTTACAGGCTTCTTGTACTGCCTGTAAGTA720.10547750545699594No Hit
CTACGAGTTTTAGTCTGAGCATGTAGTGCCTTGTAGGCCATT710.10401254010342655No Hit
CTACGAGTCTGGTCCCTTGCCACTGCCTAGCCCATTGCGGGG710.10401254010342655No Hit
CTACGAGTTCAGCGTTCGCCGCCCGGCCCCGCCGGCCGCGAG690.10108260939628778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGAAG200.002087213636.0212631
ACAACAT551.6370905E-1135.99487324
GAGTGGG1550.035.9948735
GAGTGCG1250.035.9948735
TTGGCGC309.901994E-635.9948738
TTGGCAG551.6370905E-1135.99487311
GGAGCAC551.6370905E-1135.99487319
GTTCTGA309.901994E-635.9948737
CTACTAT601.8189894E-1235.9948731
GTACAAT356.8592453E-735.9948737
CGAGTGT3500.035.9948734
CGAGTCA2250.035.9948734
CGAGTAC1100.035.9948734
GAGTCTT1700.035.9948735
CGCGGCC700.035.99487312
GAGTCGC850.035.9948735
AGTGGCG453.3305696E-935.9948736
TTATCGA309.901994E-635.9948738
AGTATCG309.901994E-635.9948736
CTGCGCG1150.035.9948731
Produced by FastQC (version 0.11.5)