FastQCFastQC Report
Thu 26 May 2016
SRR2099288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299992
Sequences flagged as poor quality0
Sequence length42
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGTAGGTGATCGAGCACGATGTCTTTTTTTTTTTTTTT17890.5963492359796261No Hit
ACGATGTCGATTCAGAGCCGAGCCCGCGTCGCCATGCCTCGG12390.4130110136270301No Hit
CCGAGGTCCTCGGGTTGGCGATGCCCTTTCCGGTGATGTCGA10090.33634230246139896No Hit
TCGATGCCTTTAGTTGTCCGTCTGCCGAAGATAGGTGCAAAC10030.3343422491266434No Hit
ACGATGTTTTTAGGCAGTTCATGCCGGCCACCGGCACCTATT7800.2600069335182272No Hit
ACGATGTCGGTAAGCGAGGACGACGACGAGGATACAGAAGAG7720.257340195738553No Hit
CCGAGGTCCTCGCGGTTGGCGATGCCCTTTCCGGTGATGTCG6900.23000613349689322No Hit
ACCATGTTTATCGAGGTTGCAGGCTCCCTGTGAGAATCTAAT6270.20900557348195953No Hit
ACGATGCTGCGCCGCCAATCGGCGCCTTGACGGCGCGCCGGT6200.20667217792474465No Hit
ACGATGTCACCGATCGACGCCGCAAAAGGTGTTGAAGATCGT6110.20367209792261126No Hit
ACGATGTGGAGCGCGCCCGGGGGCATGAAGTGGAATTGCTGG5630.18767167124456652No Hit
ACGATGTCCGCAGCCGGGCTGGCCTCCGACTACACCGCGCAG5430.1810048267953812No Hit
ACGATGTCGAGTTTGGGCGCGGCTGTGCCCGTGGCCTATTTT5410.18033814235046267No Hit
ACGATGTCACGCGATCACATCTGCACGGCCACCCCTTTCTTA5240.17467132456865517No Hit
ACGACGTCATGCGCTCCCTGGTCGAGCACTGACCCCGGCTAG4680.1560041601109363No Hit
ACGCTATCTCAGGGCTCAGTTCTACATTTGTGTTGCACGTCA4540.15133736899650657No Hit
ACGATGTCCTGCGAAGGCTGCTCATGCCACGCAGTGGTGATG4440.14800394677191392No Hit
ACTCTGTCAGCTTCCATTTATCCTCTCCATGACGTCTTTGTT4380.14600389343715833No Hit
ACGATGTGGAGCGGCGCCCGGGGGCATGAAGTGGAATTGCTG4110.13700365343075815No Hit
ACGATGCTGCGCCCGTTTCCGCCAATCGGCGCCTTGACGGCG4090.13633696898583963No Hit
AAGATCTCACTGGCTCTCGGCCAAGGTGCTGGACCCGCAGTT3820.12733672897943946No Hit
ACGATGTAGCGTGCAGCCTTTAACGGTGACGGTCTGGCCCAG3610.12033654230779488No Hit
ACGATGTCCCTGGGTGAAGGCCCTCCACAGTGGACAGAGCAA3440.11466972452598735No Hit
ACGATGTCCAGGGGCATTGAGGCAGCCAGCGCAGGGGCTTCT3430.11433638230352809No Hit
ACGATGTCGATTGCAGAGCCGAGCCCGCGTCGCCATGCCTCG3410.11366969785860956No Hit
ATGATGTGATGCCGGAAAGAGGTGTCGCTCGAAACCGGACAA3260.10866956452172058No Hit
ACGATATCCCTCGTTGAAAATCTGTTGGATTGAACCAGAATG3200.106669511186965No Hit
ACGATGTCGAGTCGGCTGTGCCCGTGGCCTATTTTTGGCACA3150.10500280007466865No Hit
ACGATGTCTCTGGGTATTAACCAACAGCAGACTTCCAGGATT3090.10300274673991307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCAA200.00209135336.0464368
CGAGTCA356.8860027E-736.0464368
TATGGGG200.00209135336.0464369
CACGCGT356.8860027E-736.0464368
CGTAGAG200.00209135336.0464368
CGCTAAA200.00209135336.0464368
CGCAAGT309.917032E-636.0464368
TATCGGC200.00209135336.0464369
GCGTAGG356.8860027E-736.0464369
ACGCGGG200.00209135336.0464369
AGCGTAG502.3646862E-1036.0464328
AGGTAAT251.4359047E-436.0464328
CGCGCAA453.3633114E-936.0464328
CGATTCT251.4359047E-436.0464328
CTCGATA251.4359047E-436.0464328
CAGTCGG453.3633114E-936.0464328
TCCGGCC551.6370905E-1136.028387
GTCGCAC1000.036.028386
TCGCACT309.9511635E-636.028387
TCGCACG309.9511635E-636.028387
Produced by FastQC (version 0.11.5)