FastQCFastQC Report
Thu 26 May 2016
SRR2099287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70780
Sequences flagged as poor quality0
Sequence length42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGTAGGTGATCGAGCACGATGTCTTTTTTTTTTTTTTT6400.9042102288782142No Hit
ACGATGTCGATTCAGAGCCGAGCCCGCGTCGCCATGCCTCGG4360.6159932184232834No Hit
TCGATGCCTTTAGTTGTCCGTCTGCCGAAGATAGGTGCAAAC3790.5354619949138175No Hit
CCGAGGTCCTCGGGTTGGCGATGCCCTTTCCGGTGATGTCGA3200.4521051144391071No Hit
ACGATGTTTTTAGGCAGTTCATGCCGGCCACCGGCACCTATT2980.42102288782141845No Hit
CCGAGGTCCTCGCGGTTGGCGATGCCCTTTCCGGTGATGTCG2240.316473580107375No Hit
ACCATGTTTATCGAGGTTGCAGGCTCCCTGTGAGAATCTAAT2170.3065837807290195No Hit
ACGATGCTGCGCCGCCAATCGGCGCCTTGACGGCGCGCCGGT2060.2910426674201752No Hit
ACGATGTCCGCAGCCGGGCTGGCCTCCGACTACACCGCGCAG2030.28680418197230856No Hit
ACGATGTCACCGATCGACGCCGCAAAAGGTGTTGAAGATCGT1920.2712630686634643No Hit
ACGATGTGGAGCGCGCCCGGGGGCATGAAGTGGAATTGCTGG1830.2585476123198644No Hit
ACGATGTCACGCGATCACATCTGCACGGCCACCCCTTTCTTA1640.23170387115004237No Hit
ACGACGTCATGCGCTCCCTGGTCGAGCACTGACCCCGGCTAG1620.22887821418479795No Hit
ACGATGTCGAGTTTGGGCGCGGCTGTGCCCGTGGCCTATTTT1600.22605255721955356No Hit
AGGCTGTCGTGAGACGAAGGTCTGGGTGTGCCCCAACCTGGC1560.22040124328906469No Hit
ACGATGTGGAGCGGCGCCCGGGGGCATGAAGTGGAATTGCTG1490.21051144391070922No Hit
ACGATGTCGGTAAGCGAGGACGACGACGAGGATACAGAAGAG1480.20909861542808703No Hit
ACGCTATCTCAGGGCTCAGTTCTACATTTGTGTTGCACGTCA1380.19497033060186494No Hit
ACGATGTCGATTGCAGAGCCGAGCCCGCGTCGCCATGCCTCG1180.16671376094942075No Hit
ACGATGCTGCGCCCGTTTCCGCCAATCGGCGCCTTGACGGCG1160.1638881039841763No Hit
AAGATCTCACTGGCTCTCGGCCAAGGTGCTGGACCCGCAGTT1150.1624752755015541No Hit
ACGATGTAGCGTGCAGCCTTTAACGGTGACGGTCTGGCCCAG1090.15399830460582084No Hit
ACGATGTCCTGCGAAGGCTGCTCATGCCACGCAGTGGTGATG1080.15258547612319864No Hit
ACGATGTCCCTGGGTGAAGGCCCTCCACAGTGGACAGAGCAA1060.14975981915795422No Hit
ACGATGTCCAGGGGCATTGAGGCAGCCAGCGCAGGGGCTTCT1060.14975981915795422No Hit
TCGATGCCTTTAGCCGAAGATAGGTGCAAACTTCCGCAAGGC1030.1455213337100876No Hit
ACGTTGTGTACTGCCTCGGCAAAGATGGCTGGTTCATGTTCG1030.1455213337100876No Hit
ACGATGTCGAGTCGGCTGTGCCCGTGGCCTATTTTTGGCACA1010.14269567674484318No Hit
ACGATATCCCTCGTTGAAAATCTGTTGGATTGAACCAGAATG1000.14128284826222096No Hit
AGGCTGTCGTGAGACGAAGGTCTTGGTGTGCCCCAACCTGGC990.13987001977959876No Hit
ATGATGTGATGCCGGAAAGAGGTGTCGCTCGAAACCGGACAA990.13987001977959876No Hit
ACGATGGCCCGGGTCACGATGTCCGACCGTAACTAGCGTACC920.1299802204012433No Hit
ACCATGTTGGGCGTCGCTAGGCCAAGAACACAAATATAGGCT910.1285673919186211No Hit
ACGATGTCCGTAGAAAGATGAGGCAGAGGTCCAAGTAAGCCA910.1285673919186211No Hit
ACGACGTCATGCGGCTCCCTGGTCGAGCACTGACCCCGGCTA900.12715456343599885No Hit
ACGATGTCGTAGGGTGGGATAGATGGGTTTTCTGGCCATGTT830.11726476405764341No Hit
ACGAGGGCTATCTTTGACCTTCGCCACGTCTATTCCTGCTGC790.11161345012715457No Hit
ACGATGTCTTGAGCGACGATCGGTTACGATGTCGCTGTAGAA780.11020062164453234No Hit
ACAATGTGGTCACATGCCGCACGGACTGGCCCAAGGTGCCTG770.10878779316191015No Hit
ACGATGTCCGCGAGGCATTAACTCATCGTAGTGCCAGCAGCA750.10596213619666574No Hit
ACGATGTCGATTTCAGAGCCGAGCCCGCGTCGCCATGCCTCG750.10596213619666574No Hit
TCGGTGTCGTCAGATTGTAGGCATTATTAATGCTTCCTTTTA750.10596213619666574No Hit
ACGATGTCTCTGGCACACGATGTCTTTTTTTTTTTTTTTTTT740.10454930771404351No Hit
ACGATGTCACTGCGGCCGGGCGCGACCCGCTCCGGGGACAGT730.1031364792314213No Hit
ATGATGTGATGCTCGGAAAGAGGTGTCGCTCGAAACCGGACA720.1017236507487991No Hit
ACGATGTCCGCAGGCCGGGCTGGCCTCCGACTACACCGCGCA710.1003108222661769No Hit
ACTCTGTCAGCTTCCATTTATCCTCTCCATGACGTCTTTGTT710.1003108222661769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAA200.002093799236.0000047
TTTAGTT404.7692083E-836.0000049
TGTCATC200.002093799236.0000045
TTTAGGC200.002093799236.0000049
GTCATCG200.002093799236.0000046
TGTCAGG251.4356943E-436.0000045
GGCAGTT200.002093799236.00000413
TTAGGCA200.002093799236.00000410
TCGCATG200.002093799236.0000047
GTCGCAT251.4356943E-436.0000046
ACCGGCA200.002093799236.00000430
TCACCTA200.002093799236.0000047
GTCGAAT200.002093799236.0000046
CGACGTC800.036.0000042
ATAGGAA200.002093799236.00000410
CTATCTC404.7692083E-836.0000044
ATGGCCC251.4356943E-436.0000044
ACTTGCG200.002093799236.0000047
CTATCAG200.002093799236.0000048
CAGCCGG251.4356943E-436.00000411
Produced by FastQC (version 0.11.5)