FastQCFastQC Report
Thu 26 May 2016
SRR2099286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846130
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCTCCCACGGAAAAGAAAGATGATACTATTGTCTTTCAT30140.35621003864654366No Hit
GATGCTCATCAGGCTGGAACCAGGCGAGCATGGGTTGGCCGT30050.3551463723068559No Hit
GATGCTCTTGGCTACCCGCCGCACCCGCGACGGCATTCACCC21720.2566981433113115No Hit
GATGCTCATTATTCCGCGATCAGCGCCACAGCGCTGCCGTGG15440.18247786983087705No Hit
GATGCTCAGTACGGGTCCTGATGCATCGAGTACATCATCGAG15390.18188694408660608No Hit
GATCCTCCTGGGGTCGCAGTCTTCCTCGGTCGCCAGACCGGC14880.17585950149504212No Hit
GATGCTCATGCGTACAGTCCGACGATCTAGTGATGCTCATTT14870.17574131634618795No Hit
GATGCTCAATTTCTGAACACTGCCTTCGAGGTGGCAGAGAAA12260.14489499249524307No Hit
GATGCTCACCTGGGACTGGGGTGGATCAAGGATATGATAAAC12140.14347677070899273No Hit
GATGTTCGCCGGCGACCTTCAAGGGTCAACCATCCAAACCGT12120.14324040041128433No Hit
GATGCTCATTATGTCCGCGATCAGCGCCACAGCGCTGCCGTG9910.11712148251450724No Hit
GATGCTCAGCCTAAAGTACAGCACAGTGCAGCTTTGGTTCAT9750.11523052013284012No Hit
GATCCTCCTGGGGGTCGCAGTCTTCCTCGGTCGCCAGACCGG9280.10967581813669294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGAG309.9337485E-636.048929
ACTAGCG309.9337485E-636.048928
AAGCCGC200.002092798236.048928
CACGTAC551.6370905E-1136.0467877
CACGCGA1150.036.0467837
GCAAGCG200.002104993736.0062712
TGTCCCG200.002104993736.0062712
CTTAGCG251.4511116E-435.99136713
GACGCGC2600.035.987111
TTAGCGC251.4541425E-435.97860314
TATTGCG200.002113563735.97647525
TGCTCAG234250.035.695563
TGCTCAC175200.035.6226353
TGCTCAA140600.035.5982933
CTCACGT12000.035.594095
GCTCACG45650.035.5666854
GCTCAAG41350.035.4738464
GATGCTC746800.035.3968051
ATGCTCA727150.035.3761062
GCTCAGG70700.035.35224
Produced by FastQC (version 0.11.5)