FastQCFastQC Report
Thu 26 May 2016
SRR2099285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189003
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCTCCCACGGAAAAGAAAGATGATACTATTGTCTTTCAT9260.4899393131325958No Hit
GATGCTCATCAGGCTGGAACCAGGCGAGCATGGGTTGGCCGT9000.4761829177314646No Hit
GATGCTCTTGGCTACCCGCCGCACCCGCGACGGCATTCACCC7600.4021100194176812No Hit
GATGCTCATGCGTACAGTCCGACGATCTAGTGATGCTCATTT5610.29682068538594625No Hit
GATGCTCATTATTCCGCGATCAGCGCCACAGCGCTGCCGTGG5500.29100067194700613No Hit
GATCCTCCTGGGGTCGCAGTCTTCCTCGGTCGCCAGACCGGC5080.2687788024528711No Hit
GATGCTCAGTACGGGTCCTGATGCATCGAGTACATCATCGAG4520.23914964312735776No Hit
GATGCTCAATTTCTGAACACTGCCTTCGAGGTGGCAGAGAAA4450.2354459982116686No Hit
GATGTTCGCCGGCGACCTTCAAGGGTCAACCATCCAAACCGT4090.21639868150240998No Hit
GATGCTCACCTGGGACTGGGGTGGATCAAGGATATGATAAAC3730.19735136479315146No Hit
GATGCTCATTATGTCCGCGATCAGCGCCACAGCGCTGCCGTG3600.19047316709258583No Hit
GATGCTCAGCCTAAAGTACAGCACAGTGCAGCTTTGGTTCAT3220.17036766612170176No Hit
GATCCTCCTGGGGGTCGCAGTCTTCCTCGGTCGCCAGACCGG3170.16772220546763808No Hit
GATGCTCATCAGGGCTGGAACCAGGCGAGCATGGGTTGGCCG2060.1089929789474241No Hit
GATGCTCATGGGAGCATCCACTTGATCCAGTCGGGCATCTCG2050.10846388681661139No Hit
GATGCTCAGGCTCCCACAGCCTCACCAGCATACGCAAAACCG1910.10105659698523303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTG200.002099328736.0116467
CATGGCA453.3815013E-936.0116467
CACGGGA356.9201997E-736.0116467
CAACGAC200.002099328736.0116467
CATGCGA356.9201997E-736.0116467
TGGACGA200.002099328736.01164614
CCGGCCC200.002099328736.01164616
CACGCGT200.002099328736.0116467
CACGCGG200.002099328736.0116467
CACGCGA251.4420658E-436.0116467
CACTGCG251.4420658E-436.0116467
CATTCGG800.036.0116467
CATTACA200.002099328736.0116467
CATAGGG251.4420658E-436.0116467
CATACTC200.002099328736.0116467
CGGGAAG356.9201997E-736.01164612
CATACCG200.002099328736.0116467
CAAGCGC251.4420658E-436.0116467
CAAGCCG200.002099328736.0116467
CATCGAA502.382876E-1036.0116467
Produced by FastQC (version 0.11.5)