FastQCFastQC Report
Thu 26 May 2016
SRR2099284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429864
Sequences flagged as poor quality0
Sequence length42
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTACAGAAGGAGTCAGTCCTTAGCAGCCCCATTTCCAATT22280.518303463420989No Hit
TGCTACAGGCGGGCGCGGCGTCGCCTTCCTCGACGACGGTTC16110.3747696946010831No Hit
TGCTACAGTCGCGATGCATCCGCCGCAGCAAAGCGGTGTTTG11990.27892542757709415No Hit
TTCGACAGTCCTGCTGGAAACCGGCGCCGACGACTATGTGGT11970.27846016414493885No Hit
TGCTACAGCTACGAACGGGCTGGGTGCTGCGGTCCAATGGAC11910.277064373848473No Hit
TGCACCAGGTTTGTCCATGCAACGCATCACCATCACCATCGA10000.23263171607764316No Hit
TGCTACAATTTGATGGCAGGGTCAGCAGCGCGGGTTCGCCGG7930.18447695084957103No Hit
TGCTACAGTCCAGCTCCACTCTCCCAGAGCCAGGCTTACTGT7710.17935905309586286No Hit
TGCTACAGGCAAAGGGAAGATGTCCTAAGCCCAGAGAACTGA7640.17773063108331938No Hit
AGCTCCAGGTCAAGATGGCATCGCTTGGTGTCACCACAGCGG7610.17703273593508645No Hit
TGCTACAGGCCGGAGACAATGAGGTGGAATGGATTCAGGTTT7520.17493905049038766No Hit
TGCTACTAGGTTGGATGCCCTCTTCTGGTATGTCTGAAGACA6930.16121377924180672No Hit
TGCTACAGCCACGCTTCCCCGAATGTGCCTTGGCCGATCTTG6430.14958219343792456No Hit
TGCTACAGTCCTAGGGTCTATAATCCTGGCAGTTTATAACTC6400.14888429828969163No Hit
TGCTACAGTGTTCATAAAACCAGCCAGTACACATTTCATTTT6380.14841903485753632No Hit
TGCTACAGAAGGGCCGGAATGGCTCAGACGTGAGTCCCCGGA6360.14795377142538105No Hit
TGCTATCGCCGCGGAGCACCATCCGGCGACCCTCGCCGGGGG6010.13981166136266354No Hit
TGCTACAGAGAAGTTGAAATCTGAGTTTCTCTATGGGCCTTA5980.1391137662144306No Hit
TGCTACAGCGTGAGGTGACCATGGAAGAACTTCAACTGTGAG5840.13585692218934362No Hit
TGCTACAGACGTATGACAGCCACTCAGATTGTCATTTGAAAA5740.13353060502856717No Hit
TGCTACAGGCATGGTTTCTCTGAATAGCCCTGGCTGTCCTGG5590.13004112928740252No Hit
TGCTACAGTCAGGCTACATGGCCCAGTTTCTCAGCAAAGTGG5410.12585375839800494No Hit
TGATCCAGTAATAAGTCAACCCCTGGAGCTGGGCACACCATA5330.12399270466938381No Hit
TGCTACAGTCGTAGTACACTTGGCAGAGGGAAGATGCCCTTT4910.11422217259412279No Hit
TTCGACAGTCCTGCTTGAAACCGGCGCCGACGACTATGTGGT4710.10956953827256993No Hit
TGCTACAATTTGGATGGCAGGGTCAGCAGCGCGGGTTCGCCG4600.10701058939571585No Hit
TGCTACAGCTTTCAAAGGAACCTTTAAAGTTTCTAGGTGTGT4390.10212532335808534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGT200.00209360536.0420729
TGCGAGC200.00209360536.0420729
GACTAGT200.00209360536.0420729
ACGCTTT502.3646862E-1036.042079
ACCCGTC251.4379658E-436.042079
ACCCCGG251.4379658E-436.042079
GGTACGC200.002094803136.0378728
GTCGCCG404.821959E-836.0378728
GGTCGGC404.821959E-836.0378728
GGTCGGA601.8189894E-1236.0378728
GGTCGAA650.036.0378728
GACGTAT650.036.0378728
GGACCGT309.94324E-636.0378728
GCGTATG200.002094803136.0378728
GCGTATC309.94324E-636.0378728
GCGTAGG309.94324E-636.0378728
GACCCCG251.438952E-436.037878
GTGCGCT251.438952E-436.037878
AGCGTAT650.036.0336727
AGCGTAA404.8268703E-836.0336727
Produced by FastQC (version 0.11.5)