FastQCFastQC Report
Thu 26 May 2016
SRR2099283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117907
Sequences flagged as poor quality0
Sequence length42
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTACAGAAGGAGTCAGTCCTTAGCAGCCCCATTTCCAATT7120.6038657586063593No Hit
TGCTACAGGCGGGCGCGGCGTCGCCTTCCTCGACGACGGTTC5110.4333924194492269No Hit
TTCGACAGTCCTGCTGGAAACCGGCGCCGACGACTATGTGGT3730.3163510224159719No Hit
TGCTACAGTCGCGATGCATCCGCCGCAGCAAAGCGGTGTTTG3530.29938850110680454No Hit
TGCTACAGCTACGAACGGGCTGGGTGCTGCGGTCCAATGGAC3530.29938850110680454No Hit
TGCACCAGGTTTGTCCATGCAACGCATCACCATCACCATCGA3490.295995996844971No Hit
TGCTACAATTTGATGGCAGGGTCAGCAGCGCGGGTTCGCCGG2900.2459565589829272No Hit
AGCTCCAGGTCAAGATGGCATCGCTTGGTGTCACCACAGCGG2570.21796839882280103No Hit
TGCTACAGGCCGGAGACAATGAGGTGGAATGGATTCAGGTTT2550.21627214669188427No Hit
TGCTACAGTCCAGCTCCACTCTCCCAGAGCCAGGCTTACTGT2540.2154240206264259No Hit
TGCTACAGGCAAAGGGAAGATGTCCTAAGCCCAGAGAACTGA2380.20185400357909197No Hit
TGCTACAGCCACGCTTCCCCGAATGTGCCTTGGCCGATCTTG2240.1899802386626748No Hit
TGCTACTAGGTTGGATGCCCTCTTCTGGTATGTCTGAAGACA2120.17980272587717439No Hit
TGCTATCGCCGCGGAGCACCATCCGGCGACCCTCGCCGGGGG1970.16708083489529885No Hit
TGCTACAGTGTTCATAAAACCAGCCAGTACACATTTCATTTT1920.162840204568007No Hit
TGCTACAGAAGGGCCGGAATGGCTCAGACGTGAGTCCCCGGA1880.1594477003061735No Hit
TGATCCAGTAATAAGTCAACCCCTGGAGCTGGGCACACCATA1870.15859957424071514No Hit
TGCTACAGAGAAGTTGAAATCTGAGTTTCTCTATGGGCCTTA1840.15605519604434No Hit
TGCTACAGCGTGAGGTGACCATGGAAGAACTTCAACTGTGAG1800.15266269178250655No Hit
TGCTACAGTCCTAGGGTCTATAATCCTGGCAGTTTATAACTC1780.15096643965158982No Hit
TGCTACAGACGTATGACAGCCACTCAGATTGTCATTTGAAAA1610.13654829653879752No Hit
TGCTACAGTCAGGCTACATGGCCCAGTTTCTCAGCAAAGTGG1540.13061141408058893No Hit
TGCTACAGTCGTAGTACACTTGGCAGAGGGAAGATGCCCTTT1510.12806703588421384No Hit
TTCGACAGTCCTGCTTGAAACCGGCGCCGACGACTATGTGGT1490.12637078375329708No Hit
TGCTACAATTTGGATGGCAGGGTCAGCAGCGCGGGTTCGCCG1430.12128202736054687No Hit
TGCTACAGCTTTCAAAGGAACCTTTAAAGTTTCTAGGTGTGT1350.11449701883687992No Hit
TGCACCAGGTTTGGTCCATGCAACGCATCACCATCACCATCG1340.11364889277142155No Hit
TGCTACAGGAGGGAAGCATTTGGTTGATCACTGAGAGATAAA1300.11025638850958806No Hit
TGCTACAGGGGTGAAGCGGTGAAAATCTTCGTAGTGAAAAAA1300.11025638850958806No Hit
TGCTACAGTCGCGGATGCATCCGCCGCAGCAAAGCGGTGTTT1260.10686388424775459No Hit
TGCTACTAGGTTGGGATGCCCTCTTCTGGTATGTCTGAAGAC1260.10686388424775459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTC251.4342289E-436.03130
CCCTCAA200.002090603636.03133
ACTCGAC251.4342289E-436.03133
GATACTA200.002094967536.01570513
CGGCCGC200.002094967536.01570513
GGCCGCG200.002094967536.01570514
AAGCCCA309.923904E-636.01570527
AAGTTCG251.4378184E-436.01570514
GTGTCAC309.923904E-636.01570528
AGTTCGT251.4378184E-436.01570515
GACGAAC251.4378184E-436.01570528
CGTCCGA309.923904E-636.01570513
ACGAACT251.4378184E-436.01570529
AAGTCAA502.3464963E-1036.01570513
CGCGGAG251.4414152E-436.00042710
TCGGCCG251.4414152E-436.00042712
GTTCGTA251.4414152E-436.00042716
CCGCGGA251.4414152E-436.0004279
CGGAGCA251.4414152E-436.00042712
CGAAGAT251.4414152E-436.00042710
Produced by FastQC (version 0.11.5)