FastQCFastQC Report
Thu 26 May 2016
SRR2099282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227848
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTATCGAGCAACTTCGTCAGCCGCACCGAGGACCTGT38871.7059618693163865No Hit
GACTACGTCTGTGCAGTCCTATCTCGATCATCAGGCGGGCAA14920.6548225132544504No Hit
GACTACGTAATGACGATCGGACGACTACGTTTTTTTTTTTTT10770.47268354341490815No Hit
GACTACGTACGAAAGTATTCTCCATTTCAGATGGTATCTATG7950.3489168217408097No Hit
GACAACCTAGGGCCAGCGTCAGCAGGTGCCGCCGCGCCGCCG7920.34760015448895754No Hit
GACTACGTGTACGCCAAGTGGGACTCGTGGAACAAGCTGAAA7540.3309223692988308No Hit
GACTACGTGGAGCAGTCCGACGATCTATTGACTACGTTTTTT7160.31424458410870404No Hit
GACTACAACCGAGGTCCCATTGAGCAGTAGGGTCTACAGGTT6980.3063445805975914No Hit
GACTACGTTTACTGTGTCTTTTCTGTCAACTCAAGGCTATTG6590.2892279063235139No Hit
GACTACATATTAATCGAGAGACTACGTTTTTTTTTTTTTTTT5520.24226677434078858No Hit
GACTACGTATACTGCTGACTACGTTTTTTTTTTTTTTTTTTT5440.23875566166918294No Hit
GACTACGCTGGTGACGATCGTTGGACTACGTTTTTTTTTTTT5100.223833432814859No Hit
GACTTCGCTTGTTTTGCGATGTACTTGACACTTCAGGAGTGG5000.219444541975352No Hit
GACTACGCTGGAGACGATCGTTGGACTACGTTTTTTTTTTTT4480.1966223096099154No Hit
GACTATTTGTGCGGACGGCCCCATTTTTCGCCCTGATGGGTT4290.18828341701485202No Hit
GACTACGCGAACAGAGCGCGCCGGCGTGGGTCGCCCCCGTCC4180.18345563709139426No Hit
GACTACGCTGGGCGTGTTGATGCGAACGCCTGCTTCGGGTGC3790.1663389628173168No Hit
GACTACGTGGGAGAATGTCTATTAAAGCCATGGATACAGGAT3660.16063340472595766No Hit
GACTACGTTGAAGAACAATCCTGCTGATGGAGCAGTTTTGAA3280.1439556195358309No Hit
GACTACGTCTGTGGCAGTCCTATCTCGATCATCAGGCGGGCA3200.14044450686422527No Hit
GACTACGTGCCTATGATGCTAGAGATTTATTCTTTTCTCTAG3200.14044450686422527No Hit
GACTACGTATGCGCCGGAAATGGTTGTGGACTACGTCTGTGC3170.13912783961237316No Hit
GACTACGTCTTTGGCATTGAGGCAGCCAGCGCAGGGGCTTCT3040.13342228152101399No Hit
GACTATTTGTGCGACGGCCCCATTTTTCGCCCTGATGGGTTT3010.1321056142691619No Hit
AACAACGTAGCTGTCCACTCCACCTGCCAGTCAGGACCCGAT2750.12069449808644359No Hit
GACTACGTATGCGACACTATCTGCTGGGGATGCTTTCTTTTT2410.10577226923211966No Hit
GACTACAACCGAAGGTCCCATTGAGCAGTAGGGTCTACAGGT2400.10533338014816894No Hit
GACTACGTTGACGCAGCTTTGCAACTTCCTTCTCGGCCGCGC2330.10226115656051403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGT551.6370905E-1136.0123066
CGTACGG551.6370905E-1136.0123066
ACGTACA2200.036.0123065
TGTCATA200.002100043536.0123028
GTGTTAC309.971132E-636.0123027
ACAACCG2350.036.0123025
CTTCGCT1200.036.0123023
CTTCGCG309.971132E-636.0123023
CGTGGTA1550.036.0123026
CGGGTCA309.971132E-636.0123026
CGTGGCA650.036.0123026
CGGAAGT309.971132E-636.0123029
AACCGAA650.036.0123027
GTCGCGA309.971132E-636.0123027
GTCGCAT200.002100043536.0123027
CGTATGG800.036.0123026
CGTATCG750.036.0123026
GGTCGTA200.002100043536.0123028
CGTATAG1300.036.0123026
GTCTGAT404.8350557E-836.0123027
Produced by FastQC (version 0.11.5)