FastQCFastQC Report
Thu 26 May 2016
SRR2099281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54295
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTATCGAGCAACTTCGTCAGCCGCACCGAGGACCTGT12432.2893452435767565No Hit
GACTACGTCTGTGCAGTCCTATCTCGATCATCAGGCGGGCAA5721.0535040058937286No Hit
GACTACGTAATGACGATCGGACGACTACGTTTTTTTTTTTTT3620.6667280596740031No Hit
GACTACGCTGGTGACGATCGTTGGACTACGTTTTTTTTTTTT3190.5875310802099641No Hit
GACAACCTAGGGCCAGCGTCAGCAGGTGCCGCCGCGCCGCCG2750.5064923105258311No Hit
GACTACGTGTACGCCAAGTGGGACTCGTGGAACAAGCTGAAA2700.49728335942536145No Hit
GACTACGTACGAAAGTATTCTCCATTTCAGATGGTATCTATG2570.47334008656414034No Hit
GACTACGTGGAGCAGTCCGACGATCTATTGACTACGTTTTTT2310.4254535408416981No Hit
GACTACGTTTACTGTGTCTTTTCTGTCAACTCAAGGCTATTG2080.3830923657795377No Hit
GACTACAACCGAGGTCCCATTGAGCAGTAGGGTCTACAGGTT2010.3701998342388802No Hit
GACTATTTGTGCGGACGGCCCCATTTTTCGCCCTGATGGGTT1530.2817939036743715No Hit
GACTTCGCTTGTTTTGCGATGTACTTGACACTTCAGGAGTGG1520.27995211345427756No Hit
GACTACGCGAACAGAGCGCGCCGGCGTGGGTCGCCCCCGTCC1470.2707431623538079No Hit
GACTACGCTGGAGACGATCGTTGGACTACGTTTTTTTTTTTT1260.23206556773183534No Hit
GACTACGTCTGTGGCAGTCCTATCTCGATCATCAGGCGGGCA1200.22101482641127176No Hit
GACTACGCTGGGCGTGTTGATGCGAACGCCTGCTTCGGGTGC1180.2173312459710839No Hit
GACTACGTTGAAGAACAATCCTGCTGATGGAGCAGTTTTGAA1100.20259692421033243No Hit
GACTACGTATGCGCCGGAAATGGTTGTGGACTACGTCTGTGC1040.19154618288976885No Hit
GACTATTTGTGCGACGGCCCCATTTTTCGCCCTGATGGGTTT970.17865365134911135No Hit
GACTACGTGCCTATGATGCTAGAGATTTATTCTTTTCTCTAG920.1694447002486417No Hit
AACAACGTAGCTGTCCACTCCACCTGCCAGTCAGGACCCGAT890.1639193295883599No Hit
GACTACGTCGTCCATCAGATCCTTCTACAAAAGGCTATACTC790.14550142738742058No Hit
GACTACGTATGCGACACTATCTGCTGGGGATGCTTTCTTTTT790.14550142738742058No Hit
GACTACGTCATTAGACTCCCACCCCCCCCAACAATTCAGGAA780.14365963716732663No Hit
GACTACGTTGACGCAGCTTTGCAACTTCCTTCTCGGCCGCGC740.13629247628695093No Hit
GACTACGTGGGAGAATGTCTATTAAAGCCATGGATACAGGAT720.13260889584676305No Hit
GACTACGTCTGGCGATCGCAGGACTCTGAACCCCTATAGTGA680.12524173496638732No Hit
GACTACAACCGAAGGTCCCATTGAGCAGTAGGGTCTACAGGT660.12155815452619947No Hit
GACTACAACCGAAGTCCCATTGAGCAGTAGGGTCTACAGGTT640.1178745740860116No Hit
GACTACGTTTGTCGCGGCGTCGCCAGGAGGAGCGCGCGGGCA640.1178745740860116No Hit
GACTACGTTGACCAGCTTTGCAACTTCCTTCTCGGCCGCGCG620.11419099364582373No Hit
GACTACGTACAGCGATCGAGGGACTGTAGAACTCTGAACCCC620.11419099364582373Illumina NlaIII expression Adapter 1 (95% over 21bp)
GACTACGTCTTTGATCTTTTTCTTTTTAAATTTTTATATTGT600.11050741320563588No Hit
GACTACGTTGAGGCCAAAGTGATGGAAAAATGCTGTGTTTAA570.10498204254535408No Hit
GACTACGTCTTTATCTTTTTCTTTTTAAATTTTTATATTGTT570.10498204254535408No Hit
GACTACGTCATCGGCACCAGGCGCTAAGGCTGGGGGATACCC560.10314025232526014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCC404.703179E-836.02675611
CAGATCC200.002081876636.02675616
TTTTGCG251.4250187E-436.02675212
GCGATCG453.274181E-936.02675212
GATCGCA251.4250187E-436.02675214
GTTTTGC251.4250187E-436.02675211
AGACGAT309.8046385E-636.02675212
ATCGCAG251.4250187E-436.02675215
CGTTCGG200.002091336835.993556
CGTTCGC309.8669825E-635.993556
CGTTCGA251.432786E-435.993556
CGTCATC356.8265945E-735.993556
CGCTGGT356.8265945E-735.993556
CGTTCAT309.8669825E-635.993556
CGTTCAG251.432786E-435.993556
CGTTCAA200.002091336835.993556
CGTTTAG356.8265945E-735.993556
CGTTTAC404.7415597E-835.993556
CGTTTAA200.002091336835.993556
GTATGGG251.432786E-435.993557
Produced by FastQC (version 0.11.5)