##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099281_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54295 Sequences flagged as poor quality 0 Sequence length 42 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.508757712496546 37.0 37.0 37.0 33.0 37.0 2 34.875955428676676 35.0 35.0 37.0 32.0 37.0 3 35.5842526936182 37.0 35.0 37.0 35.0 37.0 4 35.58064278478681 37.0 35.0 37.0 35.0 37.0 5 36.42534303342849 37.0 35.0 39.0 33.0 39.0 6 37.55649691500138 39.0 37.0 39.0 35.0 39.0 7 37.42913712128188 39.0 37.0 39.0 35.0 39.0 8 36.960125241734964 39.0 37.0 39.0 33.0 39.0 9 32.065733492955154 33.0 31.0 34.0 30.0 34.0 10 32.25287779721889 34.0 31.0 34.0 30.0 34.0 11 32.202339073579516 34.0 31.0 34.0 30.0 34.0 12 35.37618565245418 37.0 35.0 37.0 33.0 37.0 13 31.705276728980568 31.0 31.0 34.0 30.0 34.0 14 31.687153513214845 31.0 31.0 34.0 30.0 34.0 15 31.137986923289436 31.0 31.0 34.0 27.0 34.0 16 34.85604567639746 35.0 35.0 37.0 32.0 37.0 17 35.06195782300396 35.0 35.0 37.0 32.0 37.0 18 34.21344506860669 35.0 35.0 37.0 29.0 37.0 19 34.01740491757989 35.0 33.0 37.0 28.0 37.0 20 34.16220646468367 35.0 33.0 37.0 28.0 37.0 21 35.78375541025877 38.0 35.0 39.0 30.0 39.0 22 36.23125518003499 39.0 35.0 39.0 31.0 39.0 23 36.63543604383461 39.0 35.0 39.0 32.0 39.0 24 36.968984252693616 39.0 37.0 39.0 33.0 39.0 25 37.07897596463763 39.0 37.0 39.0 33.0 39.0 26 38.54500414402799 40.0 38.0 41.0 34.0 41.0 27 38.6979648218068 40.0 38.0 41.0 35.0 41.0 28 38.841071921908096 40.0 38.0 41.0 36.0 41.0 29 38.94984805230684 40.0 38.0 41.0 36.0 41.0 30 38.99033060134451 40.0 38.0 41.0 36.0 41.0 31 39.1079104889953 40.0 39.0 41.0 36.0 41.0 32 39.18646284188231 40.0 39.0 41.0 36.0 41.0 33 39.18784418454738 40.0 39.0 41.0 36.0 41.0 34 39.12139239340639 40.0 39.0 41.0 36.0 41.0 35 39.09092918316604 40.0 39.0 41.0 36.0 41.0 36 39.083967216134084 40.0 39.0 41.0 36.0 41.0 37 38.8902477207846 40.0 38.0 41.0 35.0 41.0 38 38.86717008932683 40.0 38.0 41.0 35.0 41.0 39 38.833925775854134 40.0 38.0 41.0 35.0 41.0 40 38.74358596555852 40.0 38.0 41.0 35.0 41.0 41 38.65037296251957 40.0 38.0 41.0 35.0 41.0 42 38.64528962151211 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3516913575666436 1101 2 0.35788864655143016 1101 3 0.34241725511242294 1101 4 0.1911089387267495 1101 5 0.4671735535073438 1101 6 0.12433768980643123 1101 7 0.0833075124958853 1101 8 0.31114222404546155 1101 9 0.6239757980187974 1101 10 0.6420279554477091 1101 11 0.49117341394687486 1101 12 0.6030483046814368 1101 13 0.534670094624893 1101 14 0.055030251199301716 1101 15 0.25875962979792533 1101 16 0.7684721013392775 1101 17 0.2713463456725975 1101 18 0.018219640185179742 1101 19 -0.377214776940086 1101 20 0.3979407964814712 1101 21 -0.1378301857644928 1101 22 0.047706798811837814 1101 23 0.04092538454118966 1101 24 0.12341204645883153 1101 25 0.468051100453728 1101 26 0.2973399737400584 1101 27 0.2640664582632297 1101 28 0.40309871421556664 1101 29 0.42296654058768723 1101 30 0.44539177000748253 1101 31 -0.03427897799270596 1101 32 0.1040146866710785 1101 33 0.16155537915722107 1101 34 0.31937935942295326 1101 35 0.1763946215101626 1101 36 0.3359738357574713 1101 37 0.22355933618964485 1101 38 0.01885957336831723 1101 39 0.13370892386588196 1101 40 0.279734043776358 1101 41 0.2100469447140938 1101 42 0.6108306240590835 1102 1 0.16479997179884975 1102 2 0.07723945803707011 1102 3 0.14341600667297172 1102 4 -0.3728111611484053 1102 5 0.27915857223393914 1102 6 0.06166602938196064 1102 7 0.07843859863820057 1102 8 0.44260289820276455 1102 9 -0.2277095952396273 1102 10 -0.3005438298207075 1102 11 -0.30470673586586017 1102 12 -0.14052223214752502 1102 13 -0.42325749588696837 1102 14 -0.520250647677102 1102 15 -0.234998172948643 1102 16 0.18407759447286054 1102 17 -0.321787237348623 1102 18 -1.0516929690532706 1102 19 -0.6516217682010108 1102 20 -0.22003673160841686 1102 21 -0.16704366891055855 1102 22 0.06780667396788687 1102 23 0.2549079063888797 1102 24 0.0664832075075239 1102 25 -0.005481983191714335 1102 26 0.4441564531408062 1102 27 0.07592663304475877 1102 28 -0.05507856418643087 1102 29 -0.01423695504277589 1102 30 0.3185503218177246 1102 31 0.3096660906714632 1102 32 0.3007687440992939 1102 33 0.4023793491946748 1102 34 0.25945426578999786 1102 35 0.018342187053235648 1102 36 0.33160429768006594 1102 37 0.5110749416827787 1102 38 0.5540655658776785 1102 39 0.3451945668121965 1102 40 0.1798588877214229 1102 41 0.2891980058876271 1102 42 0.4524036577669506 1103 1 0.020355527354404046 1103 2 0.21057279137041007 1103 3 -0.06769510443813687 1103 4 -0.07281116114840813 1103 5 -0.4208414277660637 1103 6 0.06166602938196064 1103 7 -0.23267251247290943 1103 8 0.10926956486943595 1103 9 -0.2277095952396273 1103 10 0.11056728129040039 1103 11 0.05084881968969768 1103 12 0.15947776785247214 1103 13 -0.6010352736647455 1103 14 -0.32025064767710276 1103 15 -0.7461092840597523 1103 16 -0.7381446277493637 1103 17 -0.9217872373486244 1103 18 -0.7516929690532663 1103 19 -0.9405106570899022 1103 20 -0.4978145093861954 1103 21 -1.1559325577994457 1103 22 -0.5321933260321074 1103 23 -0.6562032047222317 1103 24 -0.37796123693692607 1103 25 -0.4943708720806015 1103 26 -0.5113991024147495 1103 27 -0.7462955891774641 1103 28 -0.7773007864086523 1103 29 -0.5475702883761073 1103 30 -0.18144967818227542 1103 31 -0.4125561315507582 1103 32 -0.04367570034514756 1103 33 -0.21984287302755234 1103 34 -0.029434623098893553 1103 35 -0.003880035168990048 1103 36 -0.012840146764375504 1103 37 0.36663049723833296 1103 38 -0.14593443412232432 1103 39 -0.1103609887433592 1103 40 -0.020141112278572848 1103 41 -0.055246438556814326 1103 42 -0.0031518977886051402 1104 1 0.5779782663724902 1104 2 0.34364772677092503 1104 3 0.06021187230604852 1104 4 0.22899762438130722 1104 5 0.3714066342494391 1104 6 0.20481848416232395 1104 7 0.21487270716533402 1104 8 0.29376568890044297 1104 9 0.7746159861557231 1104 10 0.6128928626857544 1104 11 0.45498318661476134 1104 12 0.6119325482142344 1104 13 0.20490787879003491 1104 14 -0.2551343686073331 1104 15 -0.2026984313465725 1104 16 0.4055246228966283 1104 17 0.12188201329736614 1104 18 0.35399178546869337 1104 19 -0.48082073460928143 1104 20 -0.3363158013758607 1104 21 -0.5112297154221892 1104 22 -0.7828393208641486 1104 23 -0.24715927707364926 1104 24 -0.25599741264751685 1104 25 0.21053868864291303 1104 26 0.10384637562142984 1104 27 0.2152031188328678 1104 28 0.01598086733811499 1104 29 0.3420937943112321 1104 30 0.09942887478929663 1104 31 0.5370562715500213 1104 32 0.23048450637319462 1104 33 0.16129407787683903 1104 34 0.34472558361945005 1104 35 -0.141347735427388 1104 36 0.22178517623304117 1104 37 0.355777784060038 1104 38 0.3269337829319383 1104 39 0.12090516112744609 1104 40 0.3025979058092787 1104 41 0.2755029154483495 1104 42 0.4105431926506711 1105 1 -0.3662606342617565 1105 2 0.07673440753202243 1105 3 0.28457762283458976 1105 4 -0.004881868219115404 1105 5 3.706934460581124E-4 1105 6 0.3475246152405518 1105 7 0.054196174395777064 1105 8 -0.2323971017972326 1105 9 -0.15270959523962802 1105 10 -0.5303418096186867 1105 11 -0.19485825101737575 1105 12 -0.0796636462889353 1105 13 0.037096039466565145 1105 14 0.16611298868653535 1105 15 0.2637392007887307 1105 16 -0.21339715300188544 1105 17 -0.20158521714660793 1105 18 0.1692666269063281 1105 19 -0.8920258086050552 1105 20 -1.2541276406993234 1105 21 -1.6647709416378333 1105 22 -0.6546680735068549 1105 23 -0.2915567400757695 1105 24 -0.5229107318864195 1105 25 -0.39992642763616004 1105 26 -0.28463142564707056 1105 27 -0.1798814477633215 1105 28 -0.09421997832784257 1105 29 -0.01625715706298081 1105 30 0.05238870565610654 1105 31 0.12532265632803075 1105 32 0.39142530975586 1105 33 0.5288945007098249 1105 34 0.33521184154756867 1105 35 0.2471300658411124 1105 36 0.0336244997002737 1105 37 -0.10458162397378601 1105 38 -0.25578291897080874 1105 39 0.12196224357987262 1105 40 -0.3226663648038297 1105 41 -0.041357549667928595 1105 42 -0.12486906950577747 1106 1 -0.09503938563086933 1106 2 -0.2860804884154007 1106 3 -0.07371920082368177 1106 4 -0.2604952307601849 1106 5 -0.45417476109939514 1106 6 -0.5161117483958151 1106 7 -0.330798349152964 1106 8 -0.4477585475803636 1106 9 0.008434983073627222 1106 10 -0.3701556102758623 1106 11 -0.35490753907870953 1106 12 0.019049387665056372 1106 13 -0.028210641803969594 1106 14 -0.5154313705686668 1106 15 0.06152123802860032 1106 16 -0.8993226732647557 1106 17 -0.4841701021411282 1106 18 -0.273915191275492 1106 19 0.25145721439604074 1106 20 0.020927123813272885 1106 21 -0.5742725845732082 1106 22 -0.5529429913600907 1106 23 -0.025948853985951814 1106 24 0.13234666132278505 1106 25 0.15127839164095036 1106 26 -0.09158651874673751 1106 27 -0.31912022103824 1106 28 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0.5678066739678869 1107 23 0.610463461944434 1107 24 -0.4557390147147018 1107 25 -0.04992642763615862 1107 26 -0.011399102414749507 1107 27 0.1759266330447531 1107 28 -0.22174523085309517 1107 29 -0.1586813994872216 1107 30 0.018550321817720317 1107 31 -0.23477835377298106 1107 32 -0.265897922567369 1107 33 -0.6753984285831081 1107 34 -0.2294346230988964 1107 35 -0.21499114628009863 1107 36 -0.5239512578754884 1107 37 -0.5333695027616656 1107 38 -1.2237122119001 1107 39 -0.6325832109655778 1107 40 -0.7090300011674628 1107 41 -0.6108019941123715 1107 42 -0.7920407866774966 1108 1 0.019838731488775352 1108 2 -0.07495692439186996 1108 3 0.013700244399075245 1108 4 0.05457901973014856 1108 5 0.4528019830866512 1108 6 0.28621383299953607 1108 7 0.5637099164676584 1108 8 0.014695921458582006 1108 9 0.19322063731851102 1108 10 0.20591611849970803 1108 11 0.30382039591708576 1108 12 0.19332789705143938 1108 13 0.03048927413886915 1108 14 0.07044702674150471 1108 15 0.58799924307203 1108 16 0.45203625080360865 1108 17 0.07537038539039287 1108 18 -0.02972914476386279 1108 19 0.286621125855838 1108 20 -1.3479437083526022 1108 21 -0.8600669247245136 1108 22 -0.3177230417943875 1108 23 0.05516630432169478 1108 24 -0.058322994042868004 1108 25 -0.3476008462408089 1108 26 0.4410556779470127 1108 27 0.49427288627472876 1108 28 0.10900412315206864 1108 29 0.5746519338461127 1108 30 0.21570794455674047 1108 31 0.4440330157360677 1108 32 0.37001939009412865 1108 33 0.34734058950474633 1108 34 -0.05062325358984765 1108 35 0.20748947387493644 1108 36 -0.17356366097626363 1108 37 0.6115917375484088 1108 38 0.5478640154900845 1108 39 -0.2511878621283685 1108 40 0.37236534766974927 1108 41 0.19410756661114448 1108 42 -0.1359684352563093 1109 1 0.9682482476609167 1109 2 0.30635823198343104 1109 3 0.7196612174009402 1109 4 0.9325528235259242 1109 5 0.7067447791304886 1109 6 0.7252675619490105 1109 7 0.9439558400175159 1109 8 1.1770856568234578 1109 9 0.5584973013120944 1109 10 0.9599925686467259 1109 11 0.4826495859732205 1109 12 0.8426195303045816 1109 13 0.7349800520057528 1109 14 0.4981401569206021 1109 15 0.4642355435264527 1109 16 0.7821618856605994 1109 17 0.5674847933027181 1109 18 0.3582687167704819 1109 19 0.6962709521055004 1109 20 0.5155954522996282 1109 21 1.0605425379859952 1109 22 0.7187645283740167 1109 23 0.3798121209482659 1109 24 0.12625332245004728 1109 25 -0.241880450624663 1109 26 -0.017912512376433654 1109 27 0.5383787403244469 1109 28 0.4430057270013066 1109 29 0.5803990602828932 1109 30 0.6388568352276849 1109 31 0.2196277764952228 1109 32 0.2716499701529358 1109 33 0.4334138319532954 1109 34 0.27094851866355896 1109 35 0.7332847157888622 1109 36 0.7703016156877354 1109 37 0.6057109570084478 1109 38 0.6586632670271015 1109 39 0.5582213867355676 1109 40 0.4518895390624209 1109 41 0.23019417447000023 1109 42 0.6167714738589041 1110 1 0.09244224822161584 1110 2 -0.3639529538870434 1110 3 -0.22623168980398844 1110 4 -0.09828541589095607 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0.44763146291384004 2211 29 -0.09607977347096153 2211 30 -0.10448490853458026 2211 31 -0.030442310412546192 2211 32 -0.3222664862530067 2211 33 -0.3594092686915076 2211 34 -0.06168394559211521 2211 35 0.26874869111827593 2211 36 0.4914958965960565 2211 37 0.3682565134984941 2211 38 0.284959874820764 2211 39 0.21619727683929568 2211 40 0.03487243785692584 2211 41 0.05315464545402193 2211 42 -0.07442561052573637 2212 1 -0.12021875479290856 2212 2 -0.17020124108901769 2212 3 -0.01713330668533075 2212 4 -0.14596971295864591 2212 5 -0.20698374986343993 2212 6 -0.17903309671041967 2212 7 0.11701537766442272 2212 8 0.019007392584789784 2212 9 -0.06141746040816898 2212 10 0.057758292526358446 2212 11 0.1091509420367629 2212 12 -0.0261651784646304 2212 13 0.02905211709679989 2212 14 -0.0573292993624932 2212 15 0.07898434889904848 2212 16 0.8021799665078149 2212 17 -0.02078848578807424 2212 18 0.04069155029754512 2212 19 -0.0176642151423394 2212 20 -0.006553585540999052 2212 21 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2213 15 -0.08420452215499097 2213 16 -0.10084304044777781 2213 17 -0.20591422147560934 2213 18 0.07132290396260288 2213 19 0.2713941048148598 2213 20 -0.14860816017984746 2213 21 -0.02894843081531917 2213 22 0.2614574676186834 2213 23 -0.5577905063095301 2213 24 -0.3097072686829563 2213 25 -0.042783570493305945 2213 26 -0.4772721182877646 2213 27 -0.025660668542542453 2213 28 0.15523889613103137 2213 29 -0.21106235186817202 2213 30 -0.24414809088069234 2213 31 0.08744386844924179 2213 32 0.07378461711516593 2213 33 -0.4619063650910462 2213 34 -0.27626001992428684 2213 35 0.05881837752941976 2213 36 -0.04537982930406059 2213 37 0.06424954485738255 2213 38 0.4016846134967267 2213 39 -0.08258321096558063 2213 40 0.11239857026110656 2213 41 -0.7020718353822133 2213 42 -0.44521538985210185 2214 1 -0.9907555837567088 2214 2 -0.4449827641851485 2214 3 -0.46769510443813544 2214 4 -0.3728111611484053 2214 5 -0.587508094432728 2214 6 -0.6827784150624794 2214 7 -0.6548947346951266 2214 8 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0.1184333819696306 2215 39 0.13293403041372898 2215 40 -0.6400644839260821 2215 41 -0.10313915886332836 2215 42 -0.07288369855488952 2216 1 -0.08581731215176802 2216 2 -1.098069183938236 2216 3 -0.8886827587591242 2216 4 -0.8579963463335929 2216 5 -1.4294834030747054 2216 6 -1.1457413780254413 2216 7 -0.7252651050655032 2216 8 -0.04258228698241595 2216 9 -1.4474626816593812 2216 10 -0.7449882742651539 2216 11 -0.8047067358658602 2216 12 -1.0096580346166633 2216 13 0.22242151645870933 2216 14 0.30444071034758835 2216 15 0.16623639495259468 2216 16 0.6174109278061906 2216 17 0.7782127626513713 2216 18 -0.24922383325080233 2216 19 0.5064029231570117 2216 20 1.1132966017249188 2216 21 0.3811044792375853 2216 22 0.5110165505111013 2216 23 0.47219185700616606 2216 24 -0.07425753323322226 2216 25 -0.2486918597349259 2216 26 0.32563793462229285 2216 27 -0.06357953979475184 2216 28 0.09306958396171439 2216 29 0.11168897088315077 2216 30 0.14077254403994743 2216 31 0.2541105351159132 2216 32 -0.15108310775255518 2216 33 0.23077441092306827 2216 34 -0.024496351493958457 2216 35 -0.24585534381096608 2216 36 -0.11901298627054757 2216 37 -0.1346040706629026 2216 38 -0.2570455452334315 2216 39 0.3772933322442924 2216 40 0.08109345562266412 2216 41 -0.3453698953469413 2216 42 -0.26488029285033576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 2.0 26 11.0 27 53.0 28 131.0 29 236.0 30 479.0 31 833.0 32 1290.0 33 2221.0 34 3929.0 35 7613.0 36 11719.0 37 12334.0 38 13443.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 94.70116953678976 2.440372041624459 2.180679620591215 0.6777788009945668 2 4.956257482272769 93.77474905608251 0.9485219633483746 0.3204714982963441 3 1.5857813795008748 1.0774472787549498 0.5764803388894005 96.76029100285477 4 0.8730085643245236 4.665254627497928 93.52426558614974 0.9374712220278111 5 0.8306473892623629 96.46007919697946 1.7810111428308315 0.9282622709273414 6 1.5158212693851991 0.771724315762331 1.5323976866688769 96.18005672818359 7 89.78505516364909 3.3484979647468367 3.9176321072698137 2.9488147643342604 8 1.5802560088405933 7.008011787457409 86.88645363293121 4.525278570770789 9 26.565982134634865 23.009485219633483 29.14448844276637 21.280044202965282 10 32.92384197439912 19.64821806796206 27.387420572796756 20.040519384842067 11 40.12451419203919 20.99611353631357 20.90217531450885 17.977196957138382 12 31.033466561066803 24.68458180612602 21.057963273350154 23.22398835945702 13 34.930547879591764 26.279429645186248 14.315979514387827 24.474042960834165 14 36.51115245059216 26.480393420882987 15.17138489308014 21.837069235444716 15 22.217515424993092 28.739294594345704 28.536697670135368 20.506492310525832 16 21.25978451054425 38.34238880191546 21.75154249930933 18.64628418823096 17 21.455804615696312 26.699574530786656 18.737222109664227 33.1073987438528 18 31.051506889691254 25.149215238375948 20.151425834500035 23.647852037432763 19 23.251487409972555 27.134410285693235 25.85790860026893 23.756193704065282 20 22.035178193203794 23.88801915461829 25.925039138042177 28.15176351413574 21 24.67998894925868 24.40372041624459 25.93424808914265 24.982042545354084 22 24.654203886177363 24.70761580256009 28.505387236393776 22.132793074868772 23 22.99843447831292 26.849617828529333 25.591675108205177 24.560272584952575 24 21.327930748687724 26.18841513951561 27.483193664241647 25.000460447555025 25 24.045899948427024 23.13232152066603 28.225152877035292 24.596625653871655 26 25.039138042176994 23.18261350032231 28.719034901924672 23.05921355557602 27 23.57307302698223 25.626669122386957 28.742978174785893 22.05727967584492 28 25.02624551063634 25.19384842066489 28.195966479417994 21.58393958928078 29 26.26945390919974 23.910120637259418 25.530896030942074 24.289529422598765 30 24.9470485311723 29.35076894741689 25.094391748779817 20.607790772630995 31 20.35106461357106 26.442201429308188 29.991527296839315 23.21520666028144 32 21.34082328022838 22.374067593701078 28.980569113178007 27.30454001289253 33 24.444239801086656 23.889860944838386 30.323234183626486 21.342665070448476 34 23.317064186389167 26.897504374251774 28.59931853761857 21.186112901740493 35 25.820057095496825 24.07035638640759 30.076434294133897 20.033152223961693 36 26.92881480799337 24.5823740675937 27.31190717377291 21.17690395064002 37 22.69822267243761 26.639653743438625 29.045031770881298 21.617091813242475 38 22.022285661663137 26.842250667648955 27.947324799705314 23.188138870982595 39 24.343838063838803 24.163335973329893 28.935590223417385 22.55723573941392 40 21.93365382844302 23.828995597797057 32.43999926322964 21.797351310530292 41 22.841882309604937 25.085182797679344 31.122571139147254 20.950363753568467 42 21.40054887370379 26.032821910743557 31.68180060044573 20.88482861510692 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 5.5 9 9.0 10 9.0 11 19.5 12 30.0 13 54.5 14 79.0 15 133.5 16 188.0 17 188.0 18 259.5 19 331.0 20 439.5 21 548.0 22 548.0 23 639.0 24 730.0 25 710.5 26 691.0 27 768.0 28 845.0 29 845.0 30 957.5 31 1070.0 32 1207.0 33 1344.0 34 1344.0 35 1748.0 36 2152.0 37 2433.0 38 2714.0 39 3049.0 40 3384.0 41 3384.0 42 3326.0 43 3268.0 44 3754.0 45 4240.0 46 4395.0 47 4550.0 48 4550.0 49 4593.5 50 4637.0 51 4922.0 52 5207.0 53 5207.0 54 5230.0 55 5253.0 56 4861.0 57 4469.0 58 3430.0 59 2391.0 60 2391.0 61 1981.0 62 1571.0 63 1499.0 64 1427.0 65 1050.0 66 673.0 67 673.0 68 628.5 69 584.0 70 525.5 71 467.0 72 467.0 73 423.0 74 379.0 75 574.5 76 770.0 77 470.0 78 170.0 79 170.0 80 123.0 81 76.0 82 58.0 83 40.0 84 40.0 85 23.0 86 6.0 87 3.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0018417902200939312 7 0.0036835804401878624 8 0.0 9 0.0 10 0.0 11 0.007367160880375725 12 0.0036835804401878624 13 0.023943272861221106 14 0.0036835804401878624 15 0.0 16 0.0 17 0.0036835804401878624 18 0.020259692421033242 19 0.011050741320563587 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.005525370660281794 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.005525370660281794 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0036835804401878624 40 0.007367160880375725 41 0.0 42 0.0036835804401878624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 54295.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.85072290266139 #Duplication Level Percentage of deduplicated Percentage of total 1 58.229828387219115 22.622709273413758 2 17.568976960273062 13.65134911133622 3 9.17322461363421 10.691592227645272 4 5.153124111121646 8.008103876968413 5 3.067222906987769 5.958191362003868 6 1.9389399829335356 4.519753200110507 7 1.137764293163933 3.094207569757805 8 0.67791789134351 2.1070080117874572 9 0.526215985588319 1.8399484298738376 >10 2.3418981700957615 15.71783773828161 >50 0.09955437565184412 2.6392853853946034 >100 0.07585095287759552 5.8071645639561655 >500 0.00474068455484972 1.0535040058937286 >1k 0.00474068455484972 2.2893452435767565 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GACTACTATCGAGCAACTTCGTCAGCCGCACCGAGGACCTGT 1243 2.2893452435767565 No Hit GACTACGTCTGTGCAGTCCTATCTCGATCATCAGGCGGGCAA 572 1.0535040058937286 No Hit GACTACGTAATGACGATCGGACGACTACGTTTTTTTTTTTTT 362 0.6667280596740031 No Hit GACTACGCTGGTGACGATCGTTGGACTACGTTTTTTTTTTTT 319 0.5875310802099641 No Hit GACAACCTAGGGCCAGCGTCAGCAGGTGCCGCCGCGCCGCCG 275 0.5064923105258311 No Hit GACTACGTGTACGCCAAGTGGGACTCGTGGAACAAGCTGAAA 270 0.49728335942536145 No Hit GACTACGTACGAAAGTATTCTCCATTTCAGATGGTATCTATG 257 0.47334008656414034 No Hit GACTACGTGGAGCAGTCCGACGATCTATTGACTACGTTTTTT 231 0.4254535408416981 No Hit GACTACGTTTACTGTGTCTTTTCTGTCAACTCAAGGCTATTG 208 0.3830923657795377 No Hit GACTACAACCGAGGTCCCATTGAGCAGTAGGGTCTACAGGTT 201 0.3701998342388802 No Hit GACTATTTGTGCGGACGGCCCCATTTTTCGCCCTGATGGGTT 153 0.2817939036743715 No Hit GACTTCGCTTGTTTTGCGATGTACTTGACACTTCAGGAGTGG 152 0.27995211345427756 No Hit GACTACGCGAACAGAGCGCGCCGGCGTGGGTCGCCCCCGTCC 147 0.2707431623538079 No Hit GACTACGCTGGAGACGATCGTTGGACTACGTTTTTTTTTTTT 126 0.23206556773183534 No Hit GACTACGTCTGTGGCAGTCCTATCTCGATCATCAGGCGGGCA 120 0.22101482641127176 No Hit GACTACGCTGGGCGTGTTGATGCGAACGCCTGCTTCGGGTGC 118 0.2173312459710839 No Hit GACTACGTTGAAGAACAATCCTGCTGATGGAGCAGTTTTGAA 110 0.20259692421033243 No Hit GACTACGTATGCGCCGGAAATGGTTGTGGACTACGTCTGTGC 104 0.19154618288976885 No Hit GACTATTTGTGCGACGGCCCCATTTTTCGCCCTGATGGGTTT 97 0.17865365134911135 No Hit GACTACGTGCCTATGATGCTAGAGATTTATTCTTTTCTCTAG 92 0.1694447002486417 No Hit AACAACGTAGCTGTCCACTCCACCTGCCAGTCAGGACCCGAT 89 0.1639193295883599 No Hit GACTACGTCGTCCATCAGATCCTTCTACAAAAGGCTATACTC 79 0.14550142738742058 No Hit GACTACGTATGCGACACTATCTGCTGGGGATGCTTTCTTTTT 79 0.14550142738742058 No Hit GACTACGTCATTAGACTCCCACCCCCCCCAACAATTCAGGAA 78 0.14365963716732663 No Hit GACTACGTTGACGCAGCTTTGCAACTTCCTTCTCGGCCGCGC 74 0.13629247628695093 No Hit GACTACGTGGGAGAATGTCTATTAAAGCCATGGATACAGGAT 72 0.13260889584676305 No Hit GACTACGTCTGGCGATCGCAGGACTCTGAACCCCTATAGTGA 68 0.12524173496638732 No Hit GACTACAACCGAAGGTCCCATTGAGCAGTAGGGTCTACAGGT 66 0.12155815452619947 No Hit GACTACAACCGAAGTCCCATTGAGCAGTAGGGTCTACAGGTT 64 0.1178745740860116 No Hit GACTACGTTTGTCGCGGCGTCGCCAGGAGGAGCGCGCGGGCA 64 0.1178745740860116 No Hit GACTACGTTGACCAGCTTTGCAACTTCCTTCTCGGCCGCGCG 62 0.11419099364582373 No Hit GACTACGTACAGCGATCGAGGGACTGTAGAACTCTGAACCCC 62 0.11419099364582373 Illumina NlaIII expression Adapter 1 (95% over 21bp) GACTACGTCTTTGATCTTTTTCTTTTTAAATTTTTATATTGT 60 0.11050741320563588 No Hit GACTACGTTGAGGCCAAAGTGATGGAAAAATGCTGTGTTTAA 57 0.10498204254535408 No Hit GACTACGTCTTTATCTTTTTCTTTTTAAATTTTTATATTGTT 57 0.10498204254535408 No Hit GACTACGTCATCGGCACCAGGCGCTAAGGCTGGGGGATACCC 56 0.10314025232526014 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.005525370660281794 0.0 0.0 13 0.0 0.0 0.011050741320563587 0.0 0.0 14 0.0 0.0 0.09208951100469656 0.0 0.0 15 0.0 0.0 0.12155815452619946 0.0 0.0 16 0.0 0.0 0.12708352518648125 0.0 0.0 17 0.0 0.0 0.12708352518648125 0.0 0.0 18 0.0 0.0 0.18417902200939312 0.0 0.0 19 0.0 0.0 0.20443871443042638 0.0 0.0 20 0.0 0.0 0.2467998894925868 0.0 0.0 21 0.0 0.0 0.4254535408416981 0.0 0.0 22 0.0 0.0 0.7385578782576665 0.0 0.0 23 0.0 0.0 0.7790772630997329 0.0 0.0 24 0.0 0.0 0.9356294318077171 0.0 0.0 25 0.0 0.0 0.9835159775301593 0.0 0.0 26 0.0 0.0 1.0093010406114744 0.0 0.0 27 0.0 0.0 1.0277189428124136 0.0 0.0 28 0.0 0.0 1.0903398102956072 0.0 0.0 29 0.0 0.0 1.1400681462381435 0.0 0.0 30 0.0 0.0 1.228474076802652 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGGTCC 40 4.703179E-8 36.026756 11 CAGATCC 20 0.0020818766 36.026756 16 TTTTGCG 25 1.4250187E-4 36.026752 12 GCGATCG 45 3.274181E-9 36.026752 12 GATCGCA 25 1.4250187E-4 36.026752 14 GTTTTGC 25 1.4250187E-4 36.026752 11 AGACGAT 30 9.8046385E-6 36.026752 12 ATCGCAG 25 1.4250187E-4 36.026752 15 CGTTCGG 20 0.0020913368 35.99355 6 CGTTCGC 30 9.8669825E-6 35.99355 6 CGTTCGA 25 1.432786E-4 35.99355 6 CGTCATC 35 6.8265945E-7 35.99355 6 CGCTGGT 35 6.8265945E-7 35.99355 6 CGTTCAT 30 9.8669825E-6 35.99355 6 CGTTCAG 25 1.432786E-4 35.99355 6 CGTTCAA 20 0.0020913368 35.99355 6 CGTTTAG 35 6.8265945E-7 35.99355 6 CGTTTAC 40 4.7415597E-8 35.99355 6 CGTTTAA 20 0.0020913368 35.99355 6 GTATGGG 25 1.432786E-4 35.99355 7 >>END_MODULE