FastQCFastQC Report
Thu 26 May 2016
SRR2099280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195909
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGATCTGAGGTCCGACGATCATGCTGACGATCTTTTTTT35861.8304416846597142No Hit
TGAAGATCCCTGAGGTCGCGTTCACGCCGCTGGGCATTCTCG29641.5129473377945883No Hit
TGACGATCAGGAAAAGCCTTTGACAGACTCATGGGGCTGGGA23941.2219959266802443No Hit
TGACGATCGAACGCCATGGATTCTGAGTTTCATTTCAAAAGT9510.4854294595960369No Hit
TGACGGTGGTGCGCGGAACGAAACGCCCTCGACCGCTGCGAC6020.3072855254225176No Hit
TGACGACCAAGCGTTGAAGGTCGCCGAGGATGCCCGCTTTCA5960.30422287898973505No Hit
TGACGATCCCGAGTTGCAAGGTTTGGGAATAACGACACAACA5520.2817634718159962No Hit
TGACGATGGCGGGGGAAGGGTCGTCTTTGACCCAATTCCGGT5060.25828318249799653No Hit
TGACGATCGGCGGACTCACTATAGTGAGTCGTATTACCGGCC5010.2557309771373444No Hit
TGACGATCCCGAGGTTGCAAGGTTTGGGAATAACGACACAAC4830.24654303783899667No Hit
TGACTATCGTACCGGCCAACGATCGTACTGACTAGCAGTTGA4780.24399083247834455No Hit
TGACGATCGTTAATGTTGGCATTGCCAACATTGAAGTGGGTG3630.18529010918334532No Hit
TGACGATCTCTTGGTAAGAGCACTGACTGCTCTTCTGAAGGT3550.1812065806063019No Hit
TGATGATAAAGAAGATTACTGCTGCCAGCAGTAATCTTGACT3390.17303952345221507No Hit
TGACGATCTGAGCGTCCGACGATCATGCTGACGATCTTTTTT3290.16793511273091077No Hit
TGACGATCTGAAAGTAAACAACCCCAAGAAGCAGGAATGAAA3210.16385158415386736No Hit
TGACGATCTAAAGACTGGCGCGCCCCAGGGGTCTCTGGGCGA3130.15976805557682394No Hit
TGACGATCCGGTAGAGAAACAAACCCACAATTTGAAGAGGCA3070.15670540914404135No Hit
TGACGATCGTGAGATTATTTTACTTCTCCAATTCCAACTCTC2910.14853835198995452No Hit
TGACGATCGCGATCGTCTGCCTGAGTGATCACAGCAGTAAAT2860.14598614662930237No Hit
TGACGATCGTTACTTCAATGTTGGCATTGCCAACATTGAAGT2770.14139217698012851No Hit
TGACGATCGCGATTCGTCTGCCTGAGTGATCACAGCAGTAAA2690.13730864840308513No Hit
TGACGATCCCTGGGGTGAGCTGTGGGGGAGGTCCCTAGGGGC2460.12556850374408526No Hit
TGACGATCGTACAATTGCGTCATTTAAAGCCTAGTTAACGCA2440.12454762159982441No Hit
TGACGATCTCGTCATGGAGCTGCATGGGGAAGGCGGCAGCTC2380.12148497516704185No Hit
TGACGATCGAACGGCCATGGATTCTGAGTTTCATTTCAAAAG2340.11944321087852014No Hit
TGACTATCGTACGCCAACGATCGTACTGACTAGCAGTTGACA2330.1189327698063897No Hit
CGATGATCATGACCCCCAACAACATCACCGGCACCGGTCCGG2220.11331791801295499No Hit
TGACGATCGATGCGATCGGAGTGACGAACTCTGAACCCCTAT2220.11331791801295499No Hit
CGATGATCATGACCCCCCAACAACATCACCGGCACCGGTCCG2200.11229703586869413No Hit
TGATGATAAAGAGATTACTGCTGCCAGCAGTAATCTTGACTC2190.11178659479656372No Hit
TGAAGATCCCTGACGCCGCTGGGCATTCTCGTGGCGCTCGTC2180.1112761537244333No Hit
TGACTATCGTACAGTGAGTCGTATTACCGGCCAACGATCGTA2170.11076571265230285No Hit
TGACGATCTGGGGGCCAGGTGAATGGAAGCAGCTCAGTCATA2160.11025527158017243No Hit
TGACGATCTGGTGGGAAAGTGGACTATGGCGAGTACATGGAT2130.10872394836378114No Hit
TGAAGATCCCTGCAGGTCGCGTTCACGCCGCTGGGCATTCTC2120.10821350729165072No Hit
TGACGATCAGGATAAAGCCTTTGACAGACTCATGGGGCTGGG2090.10668218407525944No Hit
TGACGATGGCGGGGAAGGGTCGTCTTTGACCCAATTCCGGTC2050.10464041978673772No Hit
TGACGATCGGCGGGACTCACTATAGTGAGTCGTATTACCGGC2040.1041299787146073No Hit
TGACTATCGTACGGCCAACGATCGTACTGACTAGCAGTTGAC1990.10157777335395517No Hit
TGACGACCAAGCGGTTGAAGGTCGCCGAGGATGCCCGCTTTC1980.10106733228182473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA309.694639E-636.15645236
TTAACGC309.694639E-636.15645235
GAGATAG200.002058546836.15645236
TCTGCCG251.4407869E-436.0178877
ATCTCTC502.3646862E-1036.0178876
TCGTTAA900.036.0178877
TCGCAGG251.4407869E-436.0178877
ATTAGTC251.4407869E-436.0178876
ATCTATT251.4407869E-436.0178876
ATCTAGG1950.036.0178876
CAACGGA251.4407869E-436.0178878
CATGCAG309.9534045E-636.0178879
CTGAGCG601.8189894E-1236.0178878
TCGGGCG453.3778633E-936.0178877
AAGCGGT251.4407869E-436.0178879
TCTAGGG601.8189894E-1236.0178877
TCTAGCG251.4407869E-436.0178877
TCGGCCC251.4407869E-436.0178877
TCGGCAC309.9534045E-636.0178877
TCGTGTA251.4407869E-436.0178877
Produced by FastQC (version 0.11.5)