FastQCFastQC Report
Thu 26 May 2016
SRR2099279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45481
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGATCCCTGAGGTCGCGTTCACGCCGCTGGGCATTCTCG9862.1679382599327193No Hit
TGACGATCTGAGGTCCGACGATCATGCTGACGATCTTTTTTT9842.1635408192432006No Hit
TGACGATCAGGAAAAGCCTTTGACAGACTCATGGGGCTGGGA6721.4775400716782832No Hit
TGACGATCGAACGCCATGGATTCTGAGTTTCATTTCAAAAGT2010.4419427892966294No Hit
TGACGGTGGTGCGCGGAACGAAACGCCCTCGACCGCTGCGAC1890.4155581451595171No Hit
TGACGATGGCGGGGGAAGGGTCGTCTTTGACCCAATTCCGGT1820.4001671027462017No Hit
TGACGACCAAGCGTTGAAGGTCGCCGAGGATGCCCGCTTTCA1740.3825773399881269No Hit
TGACGATCGGCGGACTCACTATAGTGAGTCGTATTACCGGCC1680.3693850179195708No Hit
TGACTATCGTACCGGCCAACGATCGTACTGACTAGCAGTTGA1670.3671862975748114No Hit
TGACGATCCCGAGTTGCAAGGTTTGGGAATAACGACACAACA1460.3210131703348651No Hit
TGACGATCTCTTGGTAAGAGCACTGACTGCTCTTCTGAAGGT1320.29023108550823423No Hit
TGACGATCCCGAGGTTGCAAGGTTTGGGAATAACGACACAAC1300.2858336448187155No Hit
TGACGATCTGAAAGTAAACAACCCCAAGAAGCAGGAATGAAA1120.2462566786130472No Hit
TGACGATCGTTAATGTTGGCATTGCCAACATTGAAGTGGGTG1050.23086563619973177No Hit
TGACGATCCCACAATACGACTCACTATAGCTATTGAAGGAAG1030.22646819551021302No Hit
TGACGATCTGAGCGTCCGACGATCATGCTGACGATCTTTTTT1020.2242694751654537No Hit
TGATGATAAAGAAGATTACTGCTGCCAGCAGTAATCTTGACT1020.2242694751654537No Hit
TGACGATCTCTCCAGAAACCAGCCGCTATCCTATCGTTATTG1010.22207075482069435No Hit
TGACGATCCGGTAGAGAAACAAACCCACAATTTGAAGAGGCA890.19568611068358216No Hit
TGACGATCCGCACGATCGTCTTGACGATCGTCGGACTGTAGA870.19128866999406344No Hit
TGACTATCGTACAGTGAGTCGTATTACCGGCCAACGATCGTA860.18908994964930412No Hit
TGACTATCGTACGCCAACGATCGTACTGACTAGCAGTTGACA820.1802950682702667No Hit
TGACTATCGTACGGCCAACGATCGTACTGACTAGCAGTTGAC810.17809634792550735No Hit
TGACGATCGCGATTCGTCTGCCTGAGTGATCACAGCAGTAAA810.17809634792550735No Hit
TGACGATCGTGAGATTATTTTACTTCTCCAATTCCAACTCTC790.17369890723598866No Hit
TGACGATCGTTACTTCAATGTTGGCATTGCCAACATTGAAGT770.16930146654646994No Hit
CGATGATCATGACCCCCAACAACATCACCGGCACCGGTCCGG750.16490402585695127No Hit
TGAAGATCCCTGCAGGTCGCGTTCACGCCGCTGGGCATTCTC730.16050658516743255No Hit
CGATGATCATGACCCCCCAACAACATCACCGGCACCGGTCCG720.1583078648226732No Hit
TGACGATCAGGATAAAGCCTTTGACAGACTCATGGGGCTGGG700.1539104241331545No Hit
TGACGATGGCGGGGAAGGGTCGTCTTTGACCCAATTCCGGTC700.1539104241331545No Hit
TGACGACCAAGCGGTTGAAGGTCGCCGAGGATGCCCGCTTTC680.1495129834436358No Hit
TGAAGATCCCTGACGCCGCTGGGCATTCTCGTGGCGCTCGTC660.14511554275411712No Hit
TGACGATCGATGCGATCGGAGTGACGAACTCTGAACCCCTAT660.14511554275411712No Hit
TGACGATCCTTAAGCAAACCAATCCAGTCACAGGACTCTGTG650.14291682240935774No Hit
TGATGATAAAGAGATTACTGCTGCCAGCAGTAATCTTGACTC640.1407181020645984No Hit
TGACGGTTAGCTGGTGGACAATACAACGAATAACAGCGCTAT640.1407181020645984No Hit
TGACGATCGCGATCGTCTGCCTGAGTGATCACAGCAGTAAAT610.13412194103032035No Hit
TGACGATCGGCGGGACTCACTATAGTGAGTCGTATTACCGGC600.131923220685561No Hit
TGACGATCCGACCAGTCCGACGATCGGCCTGACGATCGTCGG590.12972450034080166No Hit
TGACGATCGTTATCAATGTTGGCATTGCCAACATTGAAGTGG580.1275257799960423No Hit
TGACTATCGTACACTGACTAGCAGTTGACAGATAGTCAGTAC580.1275257799960423No Hit
TGACTATCGTACACCCCTATAGTGAGTCGTATTACCGGCCAA580.1275257799960423No Hit
TGACGATCTTCACAGTCCGACGATCGTGTTGACGATCGTCGG560.1231283393065236No Hit
CGATGATCATGAGGCCCCCAACAACATCACCGGCACCGGTCC560.1231283393065236No Hit
TGACGATCGGCGGAGAAGAAAGATGAGGCAGAGGTCCAAGTA550.12092961896176424No Hit
TGACGATCGGGAAGTGAGCAGGACATTTTTGATTACATCCAG530.11653217827224556No Hit
TGACTATCGTACTACCGGCCAACGATCGTACTGACTAGCAGT520.1143334579274862No Hit
TGACGGTTAGCTAGTGGACAATACAACGAATAACAGCGCTAT510.11213473758272685No Hit
TGACTATCGTACGAGTCGTATTACCGGCCAACGATCGTACTG510.11213473758272685No Hit
TCACGAGCCATGTCCGACGATCCATGAGGAGTTGGTCAGTAG480.10553857654844881No Hit
TGACTATCGTACACCGGCCAACGATCGTACTGACTAGCAGTT480.10553857654844881No Hit
CGATGATCATGATCCCCCAACAACATCACCGGCACCGGTCCG480.10553857654844881No Hit
TGACGAATGTACGTCACTGCTTCCTTAGGCATCCACACCTGA470.10333985620368945No Hit
TGACGATCGAGCGGTCCCAATAGCATTGCAATTATCTCAACC460.10114113585893011No Hit
TGACGATCTTGCGCATTCTTCTCACAGAATTAAACCTTTCTT460.10114113585893011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGT200.0020771636.0308389
ATCCCCA200.0020771636.0308386
GCGGGGG200.0020771636.0308389
ATCCCAC551.6370905E-1136.0308386
AACGCCA309.761901E-636.03083810
CAGTCTA200.0020771636.0308388
ATCTCTT502.2555469E-1036.0308386
TCCCACG200.0020771636.0308387
CGGAGCG200.0020771636.0308388
ATCTTGT200.0020771636.0308386
ATCTTGC200.0020771636.0308386
AGAAGTC251.4204186E-436.03083810
CGTTACT251.4204186E-436.0308388
TCTAGAA251.4204186E-436.0308387
ATCCACG200.0020771636.0308386
ATCCACC200.0020771636.0308386
ATCCAAC200.0020771636.0308386
TGCGCGG404.6695277E-836.03083810
CTCCCGC356.738992E-736.0308386
TGGTAAG200.0020771636.03083812
Produced by FastQC (version 0.11.5)