##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099279_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45481 Sequences flagged as poor quality 0 Sequence length 42 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.98328972537983 37.0 35.0 37.0 32.0 37.0 2 35.32585035509334 37.0 35.0 37.0 32.0 37.0 3 34.519579604670085 35.0 35.0 37.0 32.0 37.0 4 35.32694971526571 37.0 35.0 37.0 33.0 37.0 5 37.132253028737274 39.0 37.0 39.0 33.0 39.0 6 34.965392141773485 37.0 34.0 39.0 30.0 39.0 7 36.36650469426794 37.0 37.0 39.0 32.0 39.0 8 37.29808051713903 39.0 37.0 39.0 34.0 39.0 9 31.652734108748707 33.0 31.0 34.0 30.0 34.0 10 31.901343418130647 33.0 31.0 34.0 30.0 34.0 11 32.00288032365164 34.0 31.0 34.0 30.0 34.0 12 35.46274268375805 37.0 35.0 37.0 33.0 37.0 13 32.009454497482466 31.0 31.0 34.0 30.0 34.0 14 32.1059343462105 31.0 31.0 34.0 30.0 34.0 15 31.844814318066884 33.0 31.0 34.0 30.0 34.0 16 35.29027505991513 37.0 35.0 37.0 33.0 37.0 17 35.34992634286845 37.0 35.0 37.0 33.0 37.0 18 34.60220751522614 37.0 35.0 37.0 30.0 37.0 19 34.26703458587102 37.0 35.0 37.0 28.0 37.0 20 34.29201204898749 37.0 35.0 37.0 28.0 37.0 21 35.808381521954225 39.0 35.0 39.0 28.0 39.0 22 36.07420681163563 39.0 35.0 39.0 30.0 39.0 23 36.38466612431565 39.0 35.0 39.0 31.0 39.0 24 36.647808975176446 39.0 35.0 39.0 32.0 39.0 25 36.63602383412854 39.0 35.0 39.0 32.0 39.0 26 38.001407181020646 40.0 37.0 41.0 32.0 41.0 27 38.18236186539434 40.0 37.0 41.0 33.0 41.0 28 38.38730458872936 40.0 37.0 41.0 33.0 41.0 29 38.527143202656056 40.0 38.0 41.0 34.0 41.0 30 38.735208108880634 40.0 38.0 41.0 35.0 41.0 31 38.93581935313647 40.0 38.0 41.0 35.0 41.0 32 39.03777401552296 40.0 38.0 41.0 36.0 41.0 33 39.141685539016294 40.0 39.0 41.0 36.0 41.0 34 39.17712891097381 40.0 39.0 41.0 36.0 41.0 35 39.21551856819331 40.0 39.0 41.0 36.0 41.0 36 39.25975682152987 40.0 39.0 41.0 36.0 41.0 37 39.18236186539434 40.0 39.0 41.0 36.0 41.0 38 39.20184252764891 40.0 39.0 41.0 36.0 41.0 39 39.15824190321233 40.0 39.0 41.0 36.0 41.0 40 39.145885094874785 40.0 39.0 41.0 36.0 41.0 41 39.11411358589301 40.0 39.0 41.0 36.0 41.0 42 39.06655526483586 40.0 39.0 41.0 36.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2791249806149594 1101 2 -0.4623063049280063 1101 3 0.5940415756811461 1101 4 0.22563884450146077 1101 5 -0.29551076836079204 1101 6 0.3101021882449686 1101 7 -0.2911271906333539 1101 8 -0.23005403864998186 1101 9 0.5753622319038953 1101 10 0.4124174490023762 1101 11 0.38346524754886246 1101 12 -0.1458388048325503 1101 13 -0.08067381199393964 1101 14 -0.19837960816119704 1101 15 -0.26981424256961617 1101 16 -0.37302510207831574 1101 17 -0.27596489560002624 1101 18 -0.3803208954475821 1101 19 -0.32959489320827373 1101 20 -0.6634747023512944 1101 21 -0.3714801967465675 1101 22 -0.171783478721089 1101 23 -0.44596672265828374 1101 24 -1.7067082693205435 1101 25 -0.5458633039345173 1101 26 -1.5076897779222307 1101 27 -1.3869611627867755 1101 28 -0.6564755359419792 1101 29 -0.6399900543841923 1101 30 -0.5142135794740241 1101 31 -0.23088475270690623 1101 32 -0.12242112605190414 1101 33 -0.5934745939993249 1101 34 0.14673127038572176 1101 35 -0.09334146275476485 1101 36 0.060593123916234504 1101 37 -0.19653726645685055 1101 38 0.1052562598647313 1101 39 -0.1175415026948059 1101 40 0.015240667742624225 1101 41 0.03953210276137753 1101 42 0.14325781604370746 1102 1 0.9888674436274627 1102 2 0.8610838465871424 1102 3 0.544799151438724 1102 4 0.9671161172287341 1102 5 1.182235443760419 1102 6 1.4677726427904219 1102 7 1.2997819002757396 1102 8 1.0720292946833538 1102 9 0.1913660197826843 1102 10 0.6150689641538918 1102 11 0.41992358088219817 1102 12 0.7409983163795744 1102 13 -0.24544653926666626 1102 14 -0.24099324452483373 1102 15 -0.4549468183271905 1102 16 -0.053896314199526785 1102 17 -0.5676315622666905 1102 18 -1.1914004409021288 1102 19 -0.8134964083597893 1102 20 -1.2430201568967476 1102 21 -0.8700597422011143 1102 22 -1.0614615090241202 1102 23 -1.5510803590219169 1102 24 -0.4316135723508481 1102 25 -0.8062799706011816 1102 26 -0.6596784142858638 1102 27 -0.7586467688473846 1102 28 -0.6782558389722837 1102 29 -1.166505205899341 1102 30 -0.47538782189826634 1102 31 -0.039596873919030884 1102 32 0.33591220728143156 1102 33 0.3463928302430972 1102 34 0.15241308856754188 1102 35 0.29681005239675073 1102 36 0.0970514572495702 1102 37 -0.21026832706291287 1102 38 0.35336232047079363 1102 39 0.15518577003246747 1102 40 0.17953991016686643 1102 41 -0.039539866935591306 1102 42 0.12289796755885618 1103 1 0.29650907735605614 1103 2 0.27478608637634494 1103 3 0.6719401264057865 1103 4 0.8683018879797189 1103 5 0.4193895939580514 1103 6 0.7813431302739531 1103 7 0.1291626644391073 1103 8 0.04304378743697157 1103 9 0.664356797121286 1103 10 0.49122179682846223 1103 11 0.44890908812857333 1103 12 0.3734093835732466 1103 13 -0.061652072863505936 1103 14 0.12725444980981848 1103 15 0.004868728444876069 1103 16 -0.008441768744980038 1103 17 0.10628148121156755 1103 18 -0.022757489650480522 1103 19 -0.5012434439329141 1103 20 -0.2739819487281068 1103 21 0.2478585713693775 1103 22 -0.1326077540834092 1103 23 -0.22631092555683097 1103 24 -0.6865542838132939 1103 25 -0.7845408401664002 1103 26 -1.3856336185019416 1103 27 -1.3554847135114159 1103 28 -1.273776260579659 1103 29 -1.0367291848189737 1103 30 -0.7764418403435869 1103 31 -0.6285296802431404 1103 32 -0.5046675028634979 1103 33 -0.324226405593528 1103 34 -0.1947542368606534 1103 35 0.05702085608581342 1103 36 0.2709645007278283 1103 37 0.16917831325329757 1103 38 0.1405823468212546 1103 39 0.09984980165302204 1103 40 0.3863910300614677 1103 41 0.17653391435558063 1103 42 0.30471614937704317 1104 1 0.37344854879680867 1104 2 0.8125098715425807 1104 3 -0.25154665961296985 1104 4 0.09144766803087379 1104 5 0.98482011399215 1104 6 0.2632271882449686 1104 7 1.326519868190175 1104 8 0.8710489025264891 1104 9 0.4476048789627214 1104 10 0.40874097841414425 1104 11 0.17482554166650743 1104 12 0.3697861951674497 1104 13 -0.13582087081746863 1104 14 0.08654686242703491 1104 15 0.3119872280186158 1104 16 -0.47412804325478675 1104 17 -0.26861195442355523 1104 18 0.0746423398465339 1104 19 -0.5189331285023897 1104 20 0.47623118000164766 1104 21 -0.3246051967465675 1104 22 -0.045864361074031024 1104 23 0.33528327734171626 1104 24 0.35671084832651445 1104 25 -0.5807897745227493 1104 26 -0.6262559543928177 1104 27 -0.7408214569044205 1104 28 -0.1693431830008052 1104 29 -0.20616652497242427 1104 30 0.07585994993774392 1104 31 0.4639681884695648 1104 32 -0.12977406722837515 1104 33 -0.004320182234621939 1104 34 -0.20988637667310428 1104 35 -0.18892969804888082 1104 36 0.21960047685740847 1104 37 0.1591612629549175 1104 38 -0.03077315189997165 1104 39 -0.16165914975363194 1104 40 -0.07759021461031779 1104 41 -0.40807819135626744 1104 42 -0.7344995368974665 1105 1 0.45033930509932674 1105 2 0.5667115522148478 1105 3 -0.28810128146171365 1105 4 0.19438884450146077 1105 5 0.7558285173534927 1105 6 -0.15820138318360222 1105 7 0.3945870950809294 1105 8 0.438695961350021 1105 9 0.3463443747610384 1105 10 0.2427745918595221 1105 11 0.2865902475488653 1105 12 0.21978619516745113 1105 13 0.38182618800605894 1105 14 0.5239418204102293 1105 15 0.47795361457323793 1105 16 0.6010820407788273 1105 17 0.5418922472571168 1105 18 0.5969112474095581 1105 19 0.1159408210774373 1105 20 -0.3741889880655833 1105 21 0.2753948032534339 1105 22 0.45455580699319853 1105 23 0.7567118487702871 1105 24 0.5999881592508842 1105 25 0.26038669606548126 1105 26 0.262399507792054 1105 27 -0.10972901992963102 1105 28 -0.3609398216562667 1105 29 0.12786708847295358 1105 30 0.49560784909740363 1105 31 0.09590096157880623 1105 32 0.01686458823380832 1105 33 0.19357897742924735 1105 34 0.4342312703857232 1105 35 0.5388013943880949 1105 36 -0.05056759036948222 1105 37 0.32176630497171743 1105 38 0.6927562598647299 1105 39 0.7110299258766233 1105 40 0.5799728105997701 1105 41 0.94444281704709 1105 42 0.7142399589008477 1106 1 0.27358024326563424 1106 2 -0.1710283198533773 1106 3 -0.5160330511845288 1106 4 -0.5444171256477901 1106 5 -0.07466188776377436 1106 6 -0.35990714011324343 1106 7 -0.7388883846632055 1106 8 -0.6344383670081939 1106 9 -0.20845306660356755 1106 10 -0.1836646405498641 1106 11 -0.25669333454068877 1106 12 -0.4846914167728471 1106 13 0.26540827755830065 1106 14 0.29835546646566513 1106 15 0.523795832057246 1106 16 -0.3119243558096585 1106 17 -0.25171116425674 1106 18 0.488382462761372 1106 19 -0.04461354992469069 1106 20 0.1294357454099 1106 21 0.4589768928056728 1106 22 0.19272211829383679 1106 23 0.7793131280879848 1106 24 0.4012674769481137 1106 25 0.09172997964757457 1106 26 0.1557896996897128 1106 27 0.4593541357206874 1106 28 0.10238640435652968 1106 29 0.4253084530784932 1106 30 0.23718567425731862 1106 31 0.11146599356175102 1106 32 0.4801908142466047 1106 33 0.4893145851051557 1106 34 0.4163208226245274 1106 35 0.1995689850064295 1106 36 0.2711808104834006 1106 37 0.5816810171252413 1106 38 0.9569353643423426 1106 39 0.7332047659619079 1106 40 0.9669664140112815 1106 41 0.989858595298692 1106 42 0.8221290847004212 1107 1 -0.16974998061495938 1107 2 0.2720686950719937 1107 3 -0.5622084243188539 1107 4 -0.18061115549853923 1107 5 0.03261423163920796 1107 6 -0.4711478117550314 1107 7 0.0994978093666461 1107 8 -0.48005403864998186 1107 9 0.20036223190389535 1107 10 -0.009457550997623798 1107 11 0.24284024754886246 1107 12 0.0729111951674497 1107 13 0.10682618800606036 1107 14 -0.15150460816119704 1107 15 -0.0041892425696161695 1107 16 0.00197489792168426 1107 17 0.45841010439997376 1107 18 0.2759291045524179 1107 19 0.48290510679172627 1107 20 0.30527529764870565 1107 21 0.5191448032534325 1107 22 -0.312408478721089 1107 23 -0.46159172265828374 1107 24 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0.8207207712190865 1108 19 0.6083217734583926 1108 20 1.1115252976487042 1108 21 0.7487281365867631 1108 22 1.1288415212789076 1108 23 0.6652832773417146 1108 24 0.7180833973461205 1108 25 0.7470533627321458 1108 26 0.483351888744437 1108 27 0.4340805038798905 1108 28 0.06477446405801857 1108 29 0.6183432789491405 1108 30 0.7337030871926444 1108 31 0.6282819139597606 1108 32 0.7625788739480939 1108 33 0.37548373933400825 1108 34 0.0342312703857246 1108 35 0.568325203911904 1108 36 0.07038479058289937 1108 37 0.5703377335431483 1108 38 0.4327562598647319 1108 39 0.34912516397186266 1108 40 0.6837823344092939 1108 41 0.8501571027613792 1108 42 0.6780494827103709 1109 1 0.22638972526739565 1109 2 0.6213334009543487 1109 3 0.6161003992105591 1109 4 0.3267417856779318 1109 5 1.16129070222744 1109 6 0.1308742470684976 1109 7 1.2382845740725301 1109 8 0.7534018437029602 1109 9 0.021134290727424343 1109 10 0.12932921370826023 1109 11 0.20423730637239146 1109 12 -0.05668439306784734 1109 13 0.21712030565311835 1109 14 0.39537039183880296 1109 15 0.2090460515480288 1109 16 -0.018245690313612783 1109 17 -0.06272960148238127 1109 18 -0.26359295427111107 1109 19 0.06930216561525526 1109 20 0.18211353294282162 1109 21 0.7636300973710775 1109 22 0.498253285984795 1109 23 0.45293033616524525 1109 24 0.1655343777382825 1109 25 0.33097493135959866 1109 26 0.05021463384247227 1109 27 0.14153148427205053 1109 28 0.5218332875874339 1109 29 0.20559818090992366 1109 30 0.35527171464362084 1109 31 0.2580858355283908 1109 32 0.5172847563010379 1109 33 0.4074445236477331 1109 34 0.5107018586210188 1109 35 0.5022467725393511 1109 36 0.4107769474456475 1109 37 0.4091612629549175 1109 38 0.46922684810002835 1109 39 0.2795173208346071 1109 40 0.2900568442132112 1109 41 0.43015710276137753 1109 42 0.5743239925142944 1110 1 0.11725659833241053 1110 2 -0.7641155154543213 1110 3 -0.42569526642411404 1110 4 -0.10824273444590915 1110 5 -0.3193594525713195 1110 6 -0.5657201801760863 1110 7 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0.4222402651474866 2215 9 -0.64714567948851 2215 10 -0.22919647504825846 2215 11 -0.1665743094131642 2215 12 0.02484948630669237 2215 13 -0.018173811993939637 2215 14 -0.09197138031309748 2215 15 -0.10703734383544017 2215 16 0.24050337893434204 2215 17 0.33321231958984754 2215 18 -0.4560724777260603 2215 19 -0.22239552611966928 2215 20 0.15456327233224698 2215 21 0.3905846766711534 2215 22 0.24732253393713677 2215 23 0.2055364418986798 2215 24 0.6752141990338885 2215 25 0.8793740378376356 2215 26 0.7994700322043542 2215 27 0.6828568751879089 2215 28 0.6121162362099213 2215 29 0.28247830004618635 2215 30 0.4695680660955972 2215 31 -0.13357462612462712 2215 32 -0.035395809596209915 2215 33 0.17531496296270177 2215 34 -0.03918645113326846 2215 35 -0.4208731083243862 2215 36 -0.3502059267166757 2215 37 -0.225611633545455 2215 38 -0.3844589300086838 2215 39 -0.23146555332771612 2215 40 -0.2654159778269971 2215 41 0.01876469769808864 2215 42 -0.4336804750955352 2216 1 0.3466763014363252 2216 2 0.23520972071302282 2216 3 0.36647747311704393 2216 4 -0.34407269396007933 2216 5 -0.09398833246335414 2216 6 -0.0893369143191336 2216 7 -0.009075908582069303 2216 8 -0.14591942326536866 2216 9 0.19835902677569095 2216 10 0.2665841156690405 2216 11 0.35582101677962896 2216 12 0.4915810669623184 2216 13 -0.15919945301958194 2216 14 0.010755007223416158 2216 15 -0.05867642205679502 2216 16 -0.26485202515523554 2216 17 0.00929151465638256 2216 18 -0.4408176903193777 2216 19 -0.42655002141340503 2216 20 0.09614068226409245 2216 21 -0.6194769916183631 2216 22 0.12268767512506429 2216 23 0.08528327734171626 2216 24 -0.11576275650003254 2216 25 -0.19089535521656842 2216 26 0.1946339400264847 2216 27 0.3602343500337355 2216 28 0.09913343841699174 2216 29 -0.16883620823034562 2216 30 -0.27142511793556423 2216 31 -0.13633347065562162 2216 32 -0.15126727989805744 2216 33 0.383688867539135 2216 34 0.5521799883344372 2216 35 0.23037648596318405 2216 36 0.1867950469931543 2216 37 0.42726081046622966 2216 38 0.2927562598647313 2216 39 0.7157918306385298 2216 40 0.5396797703067264 2216 41 0.5583622309665088 2216 42 0.6765110211719119 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 2.0 26 17.0 27 44.0 28 118.0 29 232.0 30 491.0 31 902.0 32 1588.0 33 2706.0 34 4170.0 35 5425.0 36 6321.0 37 9710.0 38 13755.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.5171170378839516 3.533343594028276 92.18794661507003 2.7615927530177435 2 93.8281919922605 3.6476770519557618 1.1697192234119742 1.3544117323717595 3 1.2334821134099954 93.78201886502056 2.5131373540599373 2.4713616675095094 4 0.7673534003210132 7.34152723115147 4.663485851234581 87.22763351729293 5 91.39401934916447 2.5857519788918206 5.5584872471416 0.46174142480211083 6 2.5109386337151776 94.79343022360986 0.8509047734218684 1.8447263692530949 7 2.0998240985048375 1.4775725593667546 94.98460861917326 1.437994722955145 8 4.859171961918164 2.1943229040698315 1.7545788351179612 91.19192629889405 9 32.34168865435356 22.510993843447668 24.643799472295512 20.503518029903255 10 33.03137573931972 20.86805479211099 25.274290363008728 20.826279105560562 11 33.69761203219139 22.956154624213905 25.836668279167952 17.509565064426756 12 31.95540799050111 24.89060885243739 17.445414367070516 25.708568789990988 13 32.116547553600874 28.888400219901044 15.544804837822978 23.4502473886751 14 36.070007255777135 25.76460499989006 18.37030848046437 19.795079263868427 15 24.859278803869834 26.354441512752857 27.40985048372911 21.376429199648197 16 21.710164684153824 32.149688881071214 25.280886524043005 20.859259910731954 17 22.486312965853873 26.208746509531455 17.871198962203998 33.43374156241068 18 30.854667018562505 29.143573502243335 15.270080056303334 24.731679422890824 19 23.658751099384347 26.893139841688658 22.491204925241863 26.95690413368514 20 26.272509399529476 25.560124007827444 24.203513555110927 23.963853037532157 21 21.703568523119547 26.065829687122093 27.433433741562414 24.79716804819595 22 23.253666366174887 24.775180844748355 29.70251313735406 22.268639651722697 23 22.514896330335745 27.950133022580857 24.612475539236165 24.922495107847233 24 20.217233570062223 28.97473670323871 26.004265517468834 24.803764209230227 25 21.294606538994305 24.148545546491942 27.171786020536047 27.385061893977703 26 29.671731052527427 24.245289241661354 24.5619049713067 21.52107473450452 27 24.003430003737826 26.811195883995513 25.406213583694292 23.779160528572373 28 22.207075482069435 25.659066423341613 27.910556056375192 24.22330203821376 29 27.3256964446692 23.739583562366704 26.738638112618457 22.196081880345638 30 22.385171829994942 28.332710362568985 24.889514302675842 24.39260350476023 31 21.134099953826873 27.70387634396781 28.420659176359358 22.74136452584596 32 24.39919966579451 26.498977595039687 27.385061893977703 21.7167608451881 33 25.217123634044984 28.328312921879466 24.99945031991381 21.455113124161738 34 27.730870712401057 25.433157431838172 26.57871591908531 20.25725593667546 35 23.693410435126754 26.817792045029794 25.03462984542996 24.45416767441349 36 22.009190651041095 26.626503375035732 27.20256810536268 24.161737868560497 37 21.905850794837406 23.81214133374376 31.037136386622986 23.244871484795848 38 25.214924913700226 22.35878718585783 31.652778083155603 20.77350981728634 39 24.399736147757256 24.560246262093226 29.083113456464382 21.956904133685136 40 24.518469656992085 25.450747581354445 29.38434476693052 20.646437994722955 41 26.395637738835998 24.058398012356808 27.706075064312568 21.839889184494623 42 25.900925661265145 27.244343791913106 27.255337393636903 19.599393153184845 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 5.5 9 10.0 10 10.0 11 25.5 12 41.0 13 73.0 14 105.0 15 118.5 16 132.0 17 132.0 18 147.5 19 163.0 20 207.5 21 252.0 22 252.0 23 283.0 24 314.0 25 349.0 26 384.0 27 479.5 28 575.0 29 575.0 30 774.5 31 974.0 32 1092.5 33 1211.0 34 1211.0 35 1415.0 36 1619.0 37 1858.0 38 2097.0 39 2434.5 40 2772.0 41 2772.0 42 2879.5 43 2987.0 44 3694.0 45 4401.0 46 4457.5 47 4514.0 48 4514.0 49 4266.0 50 4018.0 51 4178.5 52 4339.0 53 4339.0 54 3914.5 55 3490.0 56 3113.5 57 2737.0 58 2635.0 59 2533.0 60 2533.0 61 2626.0 62 2719.0 63 1891.5 64 1064.0 65 818.5 66 573.0 67 573.0 68 620.5 69 668.0 70 549.0 71 430.0 72 430.0 73 278.5 74 127.0 75 118.5 76 110.0 77 93.5 78 77.0 79 77.0 80 58.5 81 40.0 82 22.0 83 4.0 84 4.0 85 2.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.00219872034475935 6 0.0 7 0.00219872034475935 8 0.0 9 0.00219872034475935 10 0.0 11 0.006596161034278051 12 0.0043974406895187 13 0.013192322068556101 14 0.0 15 0.00219872034475935 16 0.0 17 0.0 18 0.02858336448187155 19 0.00219872034475935 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.00219872034475935 35 0.0 36 0.0 37 0.0 38 0.0 39 0.00219872034475935 40 0.00219872034475935 41 0.0 42 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 45481.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.692245113344036 #Duplication Level Percentage of deduplicated Percentage of total 1 59.07238734419943 21.67498515863767 2 16.814477468839886 12.339218574789472 3 8.587008628954939 9.452298762120446 4 5.123441994247364 7.519623579076977 5 3.175934803451582 5.826608913612278 6 1.8875838926174497 4.155581451595172 7 1.174496644295302 3.0166443130098286 8 0.6291946308724832 1.8469250895978542 9 0.5992329817833174 1.978848310283415 >10 2.636625119846596 17.020294188782128 >50 0.19175455417066153 4.711857698819287 >100 0.0898849472674976 4.648094808821266 >500 0.01797698945349952 5.809019150854203 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGAAGATCCCTGAGGTCGCGTTCACGCCGCTGGGCATTCTCG 986 2.1679382599327193 No Hit TGACGATCTGAGGTCCGACGATCATGCTGACGATCTTTTTTT 984 2.1635408192432006 No Hit TGACGATCAGGAAAAGCCTTTGACAGACTCATGGGGCTGGGA 672 1.4775400716782832 No Hit TGACGATCGAACGCCATGGATTCTGAGTTTCATTTCAAAAGT 201 0.4419427892966294 No Hit TGACGGTGGTGCGCGGAACGAAACGCCCTCGACCGCTGCGAC 189 0.4155581451595171 No Hit TGACGATGGCGGGGGAAGGGTCGTCTTTGACCCAATTCCGGT 182 0.4001671027462017 No Hit TGACGACCAAGCGTTGAAGGTCGCCGAGGATGCCCGCTTTCA 174 0.3825773399881269 No Hit TGACGATCGGCGGACTCACTATAGTGAGTCGTATTACCGGCC 168 0.3693850179195708 No Hit TGACTATCGTACCGGCCAACGATCGTACTGACTAGCAGTTGA 167 0.3671862975748114 No Hit TGACGATCCCGAGTTGCAAGGTTTGGGAATAACGACACAACA 146 0.3210131703348651 No Hit TGACGATCTCTTGGTAAGAGCACTGACTGCTCTTCTGAAGGT 132 0.29023108550823423 No Hit TGACGATCCCGAGGTTGCAAGGTTTGGGAATAACGACACAAC 130 0.2858336448187155 No Hit TGACGATCTGAAAGTAAACAACCCCAAGAAGCAGGAATGAAA 112 0.2462566786130472 No Hit TGACGATCGTTAATGTTGGCATTGCCAACATTGAAGTGGGTG 105 0.23086563619973177 No Hit TGACGATCCCACAATACGACTCACTATAGCTATTGAAGGAAG 103 0.22646819551021302 No Hit TGACGATCTGAGCGTCCGACGATCATGCTGACGATCTTTTTT 102 0.2242694751654537 No Hit TGATGATAAAGAAGATTACTGCTGCCAGCAGTAATCTTGACT 102 0.2242694751654537 No Hit TGACGATCTCTCCAGAAACCAGCCGCTATCCTATCGTTATTG 101 0.22207075482069435 No Hit TGACGATCCGGTAGAGAAACAAACCCACAATTTGAAGAGGCA 89 0.19568611068358216 No Hit TGACGATCCGCACGATCGTCTTGACGATCGTCGGACTGTAGA 87 0.19128866999406344 No Hit TGACTATCGTACAGTGAGTCGTATTACCGGCCAACGATCGTA 86 0.18908994964930412 No Hit TGACTATCGTACGCCAACGATCGTACTGACTAGCAGTTGACA 82 0.1802950682702667 No Hit TGACTATCGTACGGCCAACGATCGTACTGACTAGCAGTTGAC 81 0.17809634792550735 No Hit TGACGATCGCGATTCGTCTGCCTGAGTGATCACAGCAGTAAA 81 0.17809634792550735 No Hit TGACGATCGTGAGATTATTTTACTTCTCCAATTCCAACTCTC 79 0.17369890723598866 No Hit TGACGATCGTTACTTCAATGTTGGCATTGCCAACATTGAAGT 77 0.16930146654646994 No Hit CGATGATCATGACCCCCAACAACATCACCGGCACCGGTCCGG 75 0.16490402585695127 No Hit TGAAGATCCCTGCAGGTCGCGTTCACGCCGCTGGGCATTCTC 73 0.16050658516743255 No Hit CGATGATCATGACCCCCCAACAACATCACCGGCACCGGTCCG 72 0.1583078648226732 No Hit TGACGATCAGGATAAAGCCTTTGACAGACTCATGGGGCTGGG 70 0.1539104241331545 No Hit TGACGATGGCGGGGAAGGGTCGTCTTTGACCCAATTCCGGTC 70 0.1539104241331545 No Hit TGACGACCAAGCGGTTGAAGGTCGCCGAGGATGCCCGCTTTC 68 0.1495129834436358 No Hit TGAAGATCCCTGACGCCGCTGGGCATTCTCGTGGCGCTCGTC 66 0.14511554275411712 No Hit TGACGATCGATGCGATCGGAGTGACGAACTCTGAACCCCTAT 66 0.14511554275411712 No Hit TGACGATCCTTAAGCAAACCAATCCAGTCACAGGACTCTGTG 65 0.14291682240935774 No Hit TGATGATAAAGAGATTACTGCTGCCAGCAGTAATCTTGACTC 64 0.1407181020645984 No Hit TGACGGTTAGCTGGTGGACAATACAACGAATAACAGCGCTAT 64 0.1407181020645984 No Hit TGACGATCGCGATCGTCTGCCTGAGTGATCACAGCAGTAAAT 61 0.13412194103032035 No Hit TGACGATCGGCGGGACTCACTATAGTGAGTCGTATTACCGGC 60 0.131923220685561 No Hit TGACGATCCGACCAGTCCGACGATCGGCCTGACGATCGTCGG 59 0.12972450034080166 No Hit TGACGATCGTTATCAATGTTGGCATTGCCAACATTGAAGTGG 58 0.1275257799960423 No Hit TGACTATCGTACACTGACTAGCAGTTGACAGATAGTCAGTAC 58 0.1275257799960423 No Hit TGACTATCGTACACCCCTATAGTGAGTCGTATTACCGGCCAA 58 0.1275257799960423 No Hit TGACGATCTTCACAGTCCGACGATCGTGTTGACGATCGTCGG 56 0.1231283393065236 No Hit CGATGATCATGAGGCCCCCAACAACATCACCGGCACCGGTCC 56 0.1231283393065236 No Hit TGACGATCGGCGGAGAAGAAAGATGAGGCAGAGGTCCAAGTA 55 0.12092961896176424 No Hit TGACGATCGGGAAGTGAGCAGGACATTTTTGATTACATCCAG 53 0.11653217827224556 No Hit TGACTATCGTACTACCGGCCAACGATCGTACTGACTAGCAGT 52 0.1143334579274862 No Hit TGACGGTTAGCTAGTGGACAATACAACGAATAACAGCGCTAT 51 0.11213473758272685 No Hit TGACTATCGTACGAGTCGTATTACCGGCCAACGATCGTACTG 51 0.11213473758272685 No Hit TCACGAGCCATGTCCGACGATCCATGAGGAGTTGGTCAGTAG 48 0.10553857654844881 No Hit TGACTATCGTACACCGGCCAACGATCGTACTGACTAGCAGTT 48 0.10553857654844881 No Hit CGATGATCATGATCCCCCAACAACATCACCGGCACCGGTCCG 48 0.10553857654844881 No Hit TGACGAATGTACGTCACTGCTTCCTTAGGCATCCACACCTGA 47 0.10333985620368945 No Hit TGACGATCGAGCGGTCCCAATAGCATTGCAATTATCTCAACC 46 0.10114113585893011 No Hit TGACGATCTTGCGCATTCTTCTCACAGAATTAAACCTTTCTT 46 0.10114113585893011 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0043974406895187 0.0 0.0 13 0.0 0.0 0.01099360172379675 0.0 0.0 14 0.0 0.0 0.0351795255161496 0.0 0.0 15 0.0 0.0 0.04617312723994635 0.0 0.0 16 0.0 0.0 0.052769288274224405 0.0 0.0 17 0.0 0.0 0.1187308986170049 0.0 0.0 18 0.0 0.0 0.15610914447791385 0.0 0.0 19 0.0 0.0 0.2022822717178602 0.0 0.0 20 0.0 0.0 0.22646819551021305 0.0 0.0 21 0.0 0.0 0.29023108550823423 0.0 0.0 22 0.0 0.0 0.6222378575668961 0.0 0.0 23 0.0 0.0 0.791539324113366 0.0 0.0 24 0.0 0.0 0.930058705833205 0.0 0.0 25 0.0 0.0 1.0751742485873221 0.0 0.0 26 0.0 0.0 2.1503484971746443 0.0 0.0 27 0.0 0.0 2.431784701303841 0.0 0.0 28 0.0 0.0 2.737406829225391 0.0 0.0 29 0.0 0.0 3.0826059233526086 0.0 0.0 30 0.0 0.0 3.9576966205668302 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAAGT 20 0.00207716 36.030838 9 ATCCCCA 20 0.00207716 36.030838 6 GCGGGGG 20 0.00207716 36.030838 9 ATCCCAC 55 1.6370905E-11 36.030838 6 AACGCCA 30 9.761901E-6 36.030838 10 CAGTCTA 20 0.00207716 36.030838 8 ATCTCTT 50 2.2555469E-10 36.030838 6 TCCCACG 20 0.00207716 36.030838 7 CGGAGCG 20 0.00207716 36.030838 8 ATCTTGT 20 0.00207716 36.030838 6 ATCTTGC 20 0.00207716 36.030838 6 AGAAGTC 25 1.4204186E-4 36.030838 10 CGTTACT 25 1.4204186E-4 36.030838 8 TCTAGAA 25 1.4204186E-4 36.030838 7 ATCCACG 20 0.00207716 36.030838 6 ATCCACC 20 0.00207716 36.030838 6 ATCCAAC 20 0.00207716 36.030838 6 TGCGCGG 40 4.6695277E-8 36.030838 10 CTCCCGC 35 6.738992E-7 36.030838 6 TGGTAAG 20 0.00207716 36.030838 12 >>END_MODULE