FastQCFastQC Report
Thu 26 May 2016
SRR2099278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406763
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTAGCCGTTCTCGATCTAGCTTTTTTTTTTTTTTTTTTTT24620.17501171128327941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT1750.037.0285457
ACCGGTA308.315998E-636.99825711
TCTAGCC345800.036.8773163
CTAGCGT94200.036.8699954
TAGCACG23250.036.8692785
TCTAGCA290450.036.8487473
TAGCGCG24300.036.799975
TAGCGTC30500.036.7857325
TCTAGCG332650.036.776783
TAGCGTT24450.036.725655
CTAGCGC86800.036.7072874
TAGCCGT22700.036.70235
TAGCGTA21650.036.6864785
CTAGCCG73700.036.6755454
TAGCGAC19500.036.648765
TAGCCCG19200.036.642835
CTAGCTC105750.036.572044
CTAGCAC78450.036.4608424
CTAGCAT80100.036.4493984
TAGCCGC18550.036.4296955
Produced by FastQC (version 0.11.5)