FastQCFastQC Report
Thu 26 May 2016
SRR2099275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125788
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTAATTCCGAAACCCAGGATGGAGTCCCACATCGGGCT5210.4141889528412885No Hit
TGCACGTAAACTTAGTGGTGGCCCGTGCCTGTAATCCCAGTAC5080.40385410373008557No Hit
TGCACGTACGTGCGGGAGTCGTATTACCGGCCGACGATACGTG4450.3537698349604096No Hit
TACACGTGTCGCGGCGGCTCGCGACGCATGCCGTTAACACTGG3530.2806309027888193No Hit
TGCACGTATGCTGCTGGTACCTGTGTAGCTGTTGTACCAACAG3500.27824593760931093No Hit
TGCACGTACTGCCGAGGCCACCCCGCTTCCCTGCAGCTCTGAG3500.27824593760931093No Hit
TGCCAGTAGAATGTGCCCGGGGCGTATTGAGAGCTACGTAAAA3470.2758609724298025No Hit
TGCACGTACTCTTCGATCGTCAATGCACGTATTTTTTTTTTTT3450.27427099564346363No Hit
TACACGCAAGGGGGGCACCTGTGTGCATGCATTTGCGTCTGCA2950.23452157598499063No Hit
TACACGCAAGGGGCGCACCTGTGTGCATGCATTTGCGTCTGCA2350.18682227239482302No Hit
TACACGCAAGGGGGCACCTGTGTGCATGCATTTGCGTCTGCAT1880.14945781791585844No Hit
TGCACGTATGCTGGCTGGTACCTGTGTAGCTGTTGTACCAACA1840.1462778643431806No Hit
TGGACATACACCGAGGTAAACACGCATTGAGATACATGGTGGA1680.13355805005246923No Hit
TGCACGTATCTTCGTTCCTTCAGCTTAGCTGTTATGTCCTTTT1630.12958310808662193No Hit
TGCACGTATGTAGCACAGCCTCTGCACCCTGGTTTTGGATGTG1510.12004324736858842No Hit
TACACGCAAGGGGGGTACACACGCACCTGTGTGCATGCATTTG1500.11924825897541896No Hit
TGCACGTACTATTGTCCGACGATCTGGATTGCACGTATTTTTT1400.11129837504372438No Hit
TGCACGTAGTTTAGTGGTGTGCTGGGTGACCTGGCACAGTGTG1370.10891340986421598No Hit
TGCACGTACGAAGGGTCAGAGAGAGGAGCGGAGCCCATGAGCG1370.10891340986421598No Hit
TGCACGTATAACCAGTCCGGCTCCTGCTGGCTGGGCTCACAGT1290.10255350271886031No Hit
TGCACGTATGCAGGGTCCTGATGTATGAAGCTTATGCTCCTAT1280.10175851432569084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTC200.00183280337.01193217
AAATGGT200.00183280337.01193217
GCCCCGT251.2279268E-436.9972228
CTTCGGT251.2279268E-436.9972210
ACTATTA308.257473E-636.997228
TCGTTAC251.2279268E-436.997229
GTATTTA600.036.997226
GTATTGT600.036.997226
GTATTGG551.2732926E-1136.997226
CGTATCA2700.036.997225
CGTATAA1350.036.997225
TACTTCA501.7644197E-1036.997227
CAAGTAT308.257473E-636.997223
TACCACT251.2279268E-436.997227
ACGCATT251.2279268E-436.9972222
ACACCGG600.036.997228
CGCATTG251.2279268E-436.9972223
CTCTTCG308.257473E-636.997229
GTAGGTC251.2279268E-436.997226
ATCGTTA251.2279268E-436.997228
Produced by FastQC (version 0.11.5)