FastQCFastQC Report
Thu 26 May 2016
SRR2099274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599936
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGCAAAACGAGTCGTATTACCGGCCGACGATCAAAAAT20930.34887054619159374No Hit
CTGATGCGGACGGGCATTGCGCACGTTGTAGCGCTGATGCTC18390.30653269682099427No Hit
CTGATGCAAAACTCACTATAGTGAGTCGTATTACCGGCCGAC14870.24785977170898227No Hit
CTGATGCAACTATCCGACGATCAGCGCTGATGCATTTTTTTT12360.2060219756774056No Hit
CTGATGCAGTGAATGAGTTAGGACATGCTAATGATAGGTACC10470.17451861531896734No Hit
CTGATGCACGGTATGCAACAAATTGGCCTAATTTATTCGCAG9510.1585169084702368No Hit
CCGATGCGTCGTGATGTCGGGGCCTTCGTCGTCAACGGTCAC7950.1325141348410497No Hit
CTGATCCTACGGGGTGGGAATCTTCAACCGCGCGCACAATGC7510.12518001920204821No Hit
CTGATGCAGGGGCAGTCCGACGATCCCATCTGATGCATTTTT7440.12401322807766163No Hit
CTGATGCAAAACTGAGTCGTATTACCGGCCGACGATCAAAAA6880.11467889908256881No Hit
CTGATGCAGTCTGCAGAAAGAAAATAACTGCTGTGGAGCTTC6820.11367879240452314No Hit
CTGATGCAATGACGTATCTGATGCATTTTTTTTTTTTTTTTT6750.11251200128013655No Hit
CTGATGCAGCGCGGAGGGCGGACTTGGTTCGCTACAGGACAA6720.11201194794111373No Hit
CTGATGGAGCGGAGATTGAGCGTTTCAAGGATGCCGCGCATT6180.1030109878387028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGT200.002096194536.0353138
GGACGAA850.036.0353139
GCGATCC404.8305083E-836.0353139
ACGTCCA200.002096194536.0353138
ATAACGC251.440233E-436.0353138
AGGGCGC200.002096194536.0353138
AGTCGGT200.002096194536.0353139
CACGCTA309.960357E-636.0323037
CACGATA356.9225644E-736.0323037
CACCGTA850.036.0323037
CGGACGT650.036.0323037
GCAACGG502.382876E-1036.0292976
GCACGCT1300.036.0292976
TACACCG251.4416482E-436.0292975
CGTATTT251.448741E-435.9992510
CCGGCAT200.00210652235.9992511
GAATCGG200.00210652235.9992511
CGACCAA200.00210652235.9992510
CGACATA251.448741E-435.9992510
TTATCGG200.00210652235.9992511
Produced by FastQC (version 0.11.5)