FastQCFastQC Report
Thu 26 May 2016
SRR2099273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140174
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGCAAAACGAGTCGTATTACCGGCCGACGATCAAAAAT6880.49081855408278285No Hit
CTGATGCGGACGGGCATTGCGCACGTTGTAGCGCTGATGCTC5890.4201920470272661No Hit
CTGATGCAAAACTCACTATAGTGAGTCGTATTACCGGCCGAC4900.3495655399717494No Hit
CTGATGCAACTATCCGACGATCAGCGCTGATGCATTTTTTTT3700.2639576526317291No Hit
CTGATGCAGTGAATGAGTTAGGACATGCTAATGATAGGTACC3260.23256809394038838No Hit
CTGATGCACGGTATGCAACAAATTGGCCTAATTTATTCGCAG3160.22543410332872002No Hit
CCGATGCGTCGTGATGTCGGGGCCTTCGTCGTCAACGGTCAC3090.2204403099005522No Hit
CTGATCCTACGGGGTGGGAATCTTCAACCGCGCGCACAATGC2630.18762395308687774No Hit
CTGATGCAAAACTGAGTCGTATTACCGGCCGACGATCAAAAA2380.16978897655770686No Hit
CTGATGCAGCGCGGAGGGCGGACTTGGTTCGCTACAGGACAA2110.1505272019062023No Hit
CTGATGGAGCGGAGATTGAGCGTTTCAAGGATGCCGCGCATT2010.14339321129453395No Hit
CTGATGCAGTCTGCAGAAAGAAAATAACTGCTGTGGAGCTTC1930.13768601880519926No Hit
CTGATGCAGGACGAAAAGCCTTTTTTGGTCCAAAGGTGTGAA1860.1326922253770314No Hit
CTGATGCAATGACGTATCTGATGCATTTTTTTTTTTTTTTTT1660.11842424415369471No Hit
CTGATGCAGCCAAAAGCACAGAACAAATCTACTTCAGTAAAT1610.11485724884786053No Hit
CTGATGCAAAACCGGCCGACGATCAAAAATGCATCAGGTTTG1580.11271705166436001No Hit
CTGATGCAAAACGGCCGACGATCAAAAATGCATCAGGTTTGA1540.10986345541969267No Hit
CTGATGCAGGGGCAGTCCGACGATCCCATCTGATGCATTTTT1530.10915005635852583No Hit
CTGATGCAACTTGATGGGTTATCTGGACCAAAGGTGGGGTGG1520.108436657297359No Hit
CTGATGCAAAACAGTGAGTCGTATTACCGGCCGACGATCAAA1470.10486966199152482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC200.002089077136.0410912
CGGTCAG200.002089077136.0410912
GACGCCC356.878345E-736.02821715
ATGTCGG200.002092745436.02821414
CGCCCTG309.913665E-636.02821417
CAGGTCG200.00210009736.00257
CAGAACG251.4423177E-436.00257
AGGTAAA309.962358E-636.00258
ACACGGG251.4423177E-436.00258
CGCGGAG200.00210009736.002510
CACATCG251.4423177E-436.00257
GACGTAT200.00210009736.002511
GCACGTA502.3646862E-1036.00256
GCAAACG650.036.00256
GCACGCA309.962358E-636.00256
GCACGAA200.00210009736.00256
CCTACGG800.036.00256
GAGAGTT200.00210009736.002511
CAATTAC356.9169437E-736.00257
TCCTACG800.036.00255
Produced by FastQC (version 0.11.5)