FastQCFastQC Report
Thu 26 May 2016
SRR2099269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86513
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACCAGGACGCGGCGACTTCTCGCGTGGTGCGCGCCCGACG10761.2437437148174264No Hit
GTCACTAGGCGCGGACCCGTTCGTCCGGCACCTGGACGCTGGT7210.8334007605793349No Hit
GTCACTAGGGGCAGGGCCAGGGCTGGTTTCTAGGGAGGTAGGT6000.6935373874446615No Hit
GTCACTAGCCGGAGGCATGGACTAGATGAGTCCTCCAAGGTAC5590.6461456659692764No Hit
ATCATTAGTCGAGGTGCGCCGTCCTCAAGGAGGTGCGCGGGTG5260.6080011096598199No Hit
GTCACTAGAGCATCAGCCTGGCCAAGATGGTGAAACCCCGTCT4130.47738490169107534No Hit
GTTACGAGCGCGCGCCGGAATGGATGCGGCCGGTGATGATGCG3550.4103429542380914No Hit
GTCACTATGGGTGGGCTAACGCTCTTGGTAATCATCAGCGTGA2630.30400055482990995No Hit
GCCACCAGGACGCGGGCGACTTCTCGCGTGGTGCGCGCCCGAC2470.2855062244980523No Hit
GTCACTTGAGCTGAGCATTACCACAGCTCTGCCTTAAAAGAAA2420.2797267462693468No Hit
GTCACTAGGGACAAAGTAGACCATGTCTAGTTTTGTCTGTGGT2320.2681677898119358No Hit
GTCACTAGGCGCGGGACCCGTTCGTCCGGCACCTGGACGCTGG1700.19650225977598745No Hit
GTCACTAGCCCGTCATTGGAGCCCATGGCCAGGACTGACTTTG1580.18263151202709418No Hit
GTCACTAGTGCGGAGGAGGACCAGAGCCTGTCTCAGGGAATTG1540.1780079294441298No Hit
GGCACAAGAGTCCCCGCGCTCCTCCTCATCGGCCACGGCTTGG1410.16298128604949547No Hit
GTTACGAGCGCGCGGCCGGAATGGATGCGGCCGGTGATGATGC1290.14911053830060222No Hit
GTCACTAGGGGCAGGGGCCAGGGCTGGTTTCTAGGGAGGTAGG1170.13523979055170898No Hit
GTCACTAGGTGGTCCGACGATCAGAGTGTCACTAGTTTTTTTT1160.1340838949059679No Hit
GTCACTAGCCGGAATCCTGCAGTTTTCTCCTTTTTTTGCTTTC1140.13177210361448569No Hit
GTCACTAGGGCGCAGTACTTACTCTTCGTAAGTCCTTGCTGGT1110.1283044166772624No Hit
GTCACTAGCTTCGAGTACGAGGAGGGGAGACTGCTGGTGATTT1050.12136904280281575No Hit
GTCACTAGTAAGTCAGTCCGACGATCTTGTCGTCGGACTGTAG970.11212187763688694No Hit
GTCACTATGGGTGGGGCTAACGCTCTTGGTAATCATCAGCGTG920.10634239940818142No Hit
GTCACTAGGGCCGGTTTTCAAATCCAGTTTGGTTGAAGGATTT920.10634239940818142No Hit
GCCACCAGGACGCTGGCGACTTCTCGCGTGGTGCGCGCCCGAC910.10518650376244033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTA200.001828323537.01735315
CTTCTAA200.001828323537.01735320
TTACGGT200.001828323537.01735315
CCGCGCT200.001828323537.01735314
TACGTGG200.001828323537.01735320
GGGCGAC501.7462298E-1037.0173514
AGCGTTG251.2250003E-436.9959567
CTAGAGT1850.036.9959565
TAGAGTA650.036.9959566
CTATGGG950.036.9959565
ACTATGG1000.036.9959564
TTGTGTA251.2250003E-436.99595621
CGCGGCG1000.036.99595611
GCGCGTA251.2250003E-436.9959568
GGCACAA251.2250003E-436.9959561
TAGGTCG650.036.9959566
TAGCGTG251.2250003E-436.9959566
TAGCGGG251.2250003E-436.9959566
TAGCGCT452.562956E-936.9959566
TAGCCAC501.7462298E-1036.9959566
Produced by FastQC (version 0.11.5)