FastQCFastQC Report
Thu 26 May 2016
SRR2099268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165655
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAGCGAAGCTACGCCGACGGCTTTTAGCATTGCTTCTTTTT59330.2739586868637895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT452.5993359E-937.032888
TCGACGC40650.036.8489725
ATTCGAG526000.036.842053
TTCGACT128250.036.8281974
TTCGACC124800.036.7632684
TTCGATA123950.036.74654
TTCGAGT151000.036.723774
ATTCGAA414350.036.7068863
ATTCGAC488100.036.691013
TTCGACG130850.036.6482624
TTCGAGC132550.036.5833244
GCATTCG2013150.036.573911
TTCGAAC100850.036.5713354
TCGACGG30900.036.55185
TTCGATG149300.036.546664
TTCGAAG125750.036.544434
CATTCGA1970300.036.5369262
TTCGAAT112900.036.4563264
TCGACCG31500.036.4433755
TCGACGT33300.036.419545
Produced by FastQC (version 0.11.5)