SRR2099266_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	191471
Sequences flagged as poor quality	0
Sequence length	43
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TAGCCACGTCCAACGGTTTCGTGCCAACCAAGGTTGGCACCTA	1873	0.978216022269691	No Hit
TAGCCATGCACATCGCCGCACCAAATAGTCTGGACTTAGCTGA	1807	0.9437460503157136	No Hit
TAGCCATGGTTTGCGAGCCCAAGACCGTCTACTGGGATCGAGA	940	0.49093596419301094	No Hit
TAGCCTTTCGCTATGAGTTCCAGCCTTACATTGAAGCGAACTG	917	0.4789237012393522	No Hit
TAGCCATGTCGTGCCTAAATGCCTAGCACCATGGTACATCCAC	618	0.32276428284178804	No Hit
TAGCCACCGTGTGCGCAACCCGAAGCCGTGCCCGCTGCTCGGC	403	0.21047573784019513	No Hit
CAGCCATCAATCTGGTGAGGACCAGCAGTGATTTCTTTGAGGA	402	0.20995346553786212	No Hit
TAGGCATAATTACAACACGGACCAAGGAGTCTAACACGTGCGC	393	0.20525301481686523	No Hit
CAGCCATCAGCTCGGGCACCCTCCTCAGTATTGTACTTGTGGA	381	0.19898574718886933	No Hit
TAGCCTTGCAACGGCAGGCCGAGCAGCACCACCGCAAGGGTGG	347	0.18122848890954765	No Hit
TGGCCATGAGTTCATCACGCCCGCTATCTGCGCCTGCTGCGCC	323	0.16869395365355588	No Hit
TAGCCATGGCGTAGGGAGGGAGAGCATTTGGAAAAATAGCTAA	322	0.1681716813512229	No Hit
TAGCCATGGTGACCGGCGATGCGGACGCGATGACCGAGAAGAG	320	0.16712713674655694	No Hit
TAGCCATGCCCCGAGACAGTGTATTTCTTTTATGAAGAAAAAA	314	0.163993502932559	No Hit
TAGCCAGGTGGTTCAGAGGATGGCCTAGTAGGCCATCAATGGG	307	0.16033759681622803	No Hit
TAGCCTCGTGATTGCCCTTTGTGCGTGGCGGCTGAAACTGCGT	304	0.15877077990922905	No Hit
TAGCCATGGAAGGCTTATTCTTCTAACTACCAGTCCAAAACTA	295	0.15407032918823216	No Hit
TAGCCATAAGCTCGAATCGAACGAATCACAATATGACGGCTTG	284	0.14832533386256927	No Hit
CAGCCATCAATCTCTGCTGGTGAGGACCAGCAGTGATTTCTTT	281	0.1467585169555703	No Hit
TAGCCATGGTTTGCACAATGCCATCTTGATGGCATGAACTTGG	269	0.14049124932757442	No Hit
TAGCCATGCACATCGCCGCACCAACTAGTCTGGACTTAGCTGA	260	0.1357907986065775	No Hit
TAGTCCTGAAAGGAGCAGCTTTGTATCCTTTACAAACCCTGGT	247	0.12900125867624862	No Hit
TAGCCTCGTGATTCCCTTTGTGCGTGGCGGCTGAAACTGCGTG	237	0.12377853565291871	No Hit
TAGCCATGCGATCGGCCAAGGTGGGGCTAAGGAACCAGCCCCT	223	0.11646672342025685	No Hit
TAGCCATGGGGGCCACACACACACAAAGACAAACATTGGTGAG	203	0.10602127737359705	No Hit
TAGCCATGGCGAGGGGGAGACTCATCAAGGGAGTTTCCATTCT	198	0.1034099158619321	No Hit
TAGCAATGGTCGGGGTGGTTTAAAACCTGAAAATATTGAAGAC	193	0.10079855435026713	No Hit
TAGCCATGGTTTGCGCGAGCCCAAGACCGTCTACTGGGATCGA	192	0.10027628204793415	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGC	35	5.406255E-7	37.165398	37
TGTCATA	35	5.541242E-7	37.04868	7
TCGTTAG	25	1.22029865E-4	37.04868	9
TGCCCCG	45	2.562956E-9	37.04868	7
CAACGGC	65	0.0	37.04868	9
GCGAATC	35	5.541242E-7	37.04868	8
GACGTTT	65	0.0	37.04868	8
AGGCGTC	25	1.22029865E-4	37.04868	9
TGCGGTT	150	0.0	37.04868	7
GCGTTCG	20	0.0018262692	37.04868	8
TGCGCTC	25	1.22029865E-4	37.04868	7
TGTCTTA	25	1.22029865E-4	37.04868	7
GTTCGTA	30	8.201439E-6	37.04868	8
TCAGCGC	55	1.2732926E-11	37.04868	9
CGCTATG	130	0.0	37.04868	9
TCGCTAT	150	0.0	37.04868	8
GAGTTGT	40	3.7609425E-8	37.04868	8
CAGCTCG	50	1.7462298E-10	37.04868	8
GCGTGTC	20	0.0018262692	37.04868	9
AGCTCGA	40	3.7609425E-8	37.04868	9

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content