FastQCFastQC Report
Thu 26 May 2016
SRR2099265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46222
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCACGTCCAACGGTTTCGTGCCAACCAAGGTTGGCACCTA5921.2807753883432131No Hit
TAGCCATGCACATCGCCGCACCAAATAGTCTGGACTTAGCTGA3950.8545714162087317No Hit
TAGCCATGGTTTGCGAGCCCAAGACCGTCTACTGGGATCGAGA3130.6771667171476786No Hit
TAGCCTTTCGCTATGAGTTCCAGCCTTACATTGAAGCGAACTG2940.6360607502920687No Hit
TAGCCATGTCGTGCCTAAATGCCTAGCACCATGGTACATCCAC1810.3915884210981784No Hit
TAGCCACCGTGTGCGCAACCCGAAGCCGTGCCCGCTGCTCGGC1740.3764441175197958No Hit
TAGGCATAATTACAACACGGACCAAGGAGTCTAACACGTGCGC1400.30288607156765174No Hit
CAGCCATCAATCTGGTGAGGACCAGCAGTGATTTCTTTGAGGA1250.27043399247111766No Hit
TAGCCTTGCAACGGCAGGCCGAGCAGCACCACCGCAAGGGTGG1190.257453160832504No Hit
TGGCCATGAGTTCATCACGCCCGCTATCTGCGCCTGCTGCGCC1150.24879927307342822No Hit
TAGCCATGGTGACCGGCGATGCGGACGCGATGACCGAGAAGAG1040.2250010817359699No Hit
TAGCCTCGTGATTGCCCTTTGTGCGTGGCGGCTGAAACTGCGT1030.22283760979620093No Hit
TAGCCAGGTGGTTCAGAGGATGGCCTAGTAGGCCATCAATGGG980.21202025009735623No Hit
TAGCCATGGTTTGCACAATGCCATCTTGATGGCATGAACTTGG980.21202025009735623No Hit
TAGCCATAAGCTCGAATCGAACGAATCACAATATGACGGCTTG970.2098567781575873No Hit
CAGCCATCAATCTCTGCTGGTGAGGACCAGCAGTGATTTCTTT910.19687594651897367No Hit
TAGCCTCGTGATTCCCTTTGTGCGTGGCGGCTGAAACTGCGTG840.18173164294059105No Hit
TAGTCCTGAAAGGAGCAGCTTTGTATCCTTTACAAACCCTGGT820.1774046990610532No Hit
TAGCCATGCCCCGAGACAGTGTATTTCTTTTATGAAGAAAAAA800.1730777551815153No Hit
TAGCAATGGTCGGGGTGGTTTAAAACCTGAAAATATTGAAGAC620.13413526026567435No Hit
TAGCCATGGTTTGCGCGAGCCCAAGACCGTCTACTGGGATCGA620.13413526026567435No Hit
TAGCCATGCACATCGCCGCACCAACTAGTCTGGACTTAGCTGA620.13413526026567435No Hit
TAGCCATGGGGGCCACACACACACAAAGACAAACATTGGTGAG610.13197178832590542No Hit
TAGCCGTTCATTGATACATGCCGACGGGCGCTGACCCCCCTTC560.1211544286270607No Hit
TAGCCATGGCGTAGGGAGGGAGAGCATTTGGAAAAATAGCTAA560.1211544286270607No Hit
TAGCCATGTGTTCTGAAAGGACCACGTTTTATTTATTGTGCTT560.1211544286270607No Hit
TAGCCATGGTATAGCCAAGGGAATAAGGAACGTTCCATATCGC550.11899095668729176No Hit
TAGCCATGTTAAGAGAAATCAGGCATTTACTAAGAACCCCTGG540.11682748474752282No Hit
TAGCCATGTTCGTCTTGCAAGTCCAGAAAAACGCCACATTCTT540.11682748474752282No Hit
TAGCCATGGCGAGGGGGAGACTCATCAAGGGAGTTTCCATTCT520.11250054086798494No Hit
CAGCCATCAGCTCGGGCACCCTCCTCAGTATTGTACTTGTGGA510.110337068928216No Hit
TAGCCACCAGGGGCGCGCTCGGAGCTGGCGTAGCCCACCCGGT510.110337068928216No Hit
TAGCCATGAAAAGGGTCCAAACTGTGTCTTTCTCAGCTTTGAA470.10168318116914024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCA850.037.0000046
CATCCTA850.037.0000045
CCATGCC1050.037.0000044
CCATGAG2050.037.0000044
CATGGTT2050.037.0000045
AGACATC850.037.0000042
TAGACAT850.037.0000041
ATGGGTC200.001823713237.06
CAGCCAT600.037.01
CTCGAAT200.001823713237.011
TGCACTG200.001823713237.019
ATGGGGC600.037.06
TAGCGAT355.476977E-737.01
TGGTCGG403.698915E-837.07
ATGGGCC403.698915E-837.06
ACACGGG308.1404905E-637.04
CAATGGT355.476977E-737.04
CCGTGTG750.037.07
GGTGACG308.1404905E-637.08
CATGAGG355.476977E-737.05
Produced by FastQC (version 0.11.5)