FastQCFastQC Report
Thu 26 May 2016
SRR2099264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415888
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAAGCCGTGCGGGTCGCCGACCTGGACCCCACCTTCCTGAC23400.5626514830916016No Hit
CGTAAGCTAGTGCGGCAAACGCACTGATCCCGGGTGGAGAAGG13160.31643134690108876No Hit
CGTAAGCTATACCGCCTACCTCTCACCTCTCCCGGGTCACGGC12450.2993594429269419No Hit
CGTAAGCTGATCAGTTCTACAGTCCGTGTCGTAAGCTTTTTTT11790.2834897857115377No Hit
CGTAAGCTGCCGCTTCCCGGCTGAGCTGAATAAGGGACCTCTC11560.27795945062132116No Hit
CGTAAGCTAAGGATCAGCATAGAAATATGCTCCAACAGCAGCT11410.2743527103450929No Hit
CGTAAGCTTTCGCGGAAAGAGATCCAGGACTTCTTCGATTGTT10820.2601661985919286No Hit
CGTAAGCTATCGCATTCGGGAGGCTGAGGCAGGAGAATCACTT8600.206786442503751No Hit
CGTCAGCCAGTGTTAGAGCTTGGTGCCGGTGGTCGGCATCAAC7990.1921190320470896No Hit
CGTAAGCTTTGTCAATTAGCCGGGCGTGGTGGCGGGCGCCTGT6680.16062016696802983No Hit
CGCACGCTAGTGGCAGTCCGACGACCGTCCCAGCTAGTCTTTT6380.15340668641557342No Hit
CGTAAGCTAGGGAAGCCCAGATTGGGAACCCAACGTAAGCATT5480.13176624475820414No Hit
CGTAAGCTGATCAAGTTCTACAGTCCGTGTCGTAAGCTTTTTT5450.1310448967029585No Hit
CGTAAGCTGCCGCGTTCCCGGCTGAGCTGAATAAGGGACCTCT5200.1250336629092448No Hit
CGTAAACTCCACGGGTAAGCTTTTTTTTTTTTTTTTTTTTTTT4980.11974377717077675No Hit
CGTAAGCTTCCCAGGCAGGTTCCACCAGACAGGATGGGACGGG4520.10868310699034356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGT251.2271135E-437.0263678
TAGCTCG251.2271135E-437.0263678
TCGGTTG355.5884993E-737.0263678
TAGTCGA355.5884993E-737.0263678
GTACGAC308.258752E-637.0263679
GGTCGCT200.001834156337.0263639
TTTGTCG200.001834156337.0263638
TAACGTG200.001834156337.0263638
CGTTCGG200.001834156337.0263639
TGCGTTG200.001834156337.0263638
ACGCGCT200.001834156337.0263639
CTATACG251.2279833E-437.021917
GCTACGG1250.037.021916
GCTACCG1250.037.021916
CCGTGCG3050.037.0219087
CTTTACG452.5956979E-937.0219087
GCCGGTC200.001841760736.9951911
GAGCGGG251.2332134E-436.9951910
CGCGTCA403.8276994E-836.9951910
CGTTAGC600.036.9818421
Produced by FastQC (version 0.11.5)