FastQCFastQC Report
Thu 26 May 2016
SRR2099263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83338
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAAGCCGTGCGGGTCGCCGACCTGGACCCCACCTTCCTGAC7340.8807506779620342No Hit
CGTAAGCTATACCGCCTACCTCTCACCTCTCCCGGGTCACGGC4430.5315702320670043No Hit
CGTAAGCTAAGGATCAGCATAGAAATATGCTCCAACAGCAGCT3840.46077419664498787No Hit
CGTAAGCTTTCGCGGAAAGAGATCCAGGACTTCTTCGATTGTT3800.45597446542993597No Hit
CGTAAGCTGATCAGTTCTACAGTCCGTGTCGTAAGCTTTTTTT3680.44157527178478007No Hit
CGTAAGCTGCCGCTTCCCGGCTGAGCTGAATAAGGGACCTCTC3640.43677554056972806No Hit
CGTAAGCTAGTGCGGCAAACGCACTGATCCCGGGTGGAGAAGG3350.4019774892606014No Hit
CGTCAGCCAGTGTTAGAGCTTGGTGCCGGTGGTCGGCATCAAC2880.3455806474837409No Hit
CGCACGCTAGTGGCAGTCCGACGACCGTCCCAGCTAGTCTTTT2600.3119825289783772No Hit
CGTAAGCTATCGCATTCGGGAGGCTGAGGCAGGAGAATCACTT2350.2819842088843025No Hit
CGTAAGCTTTGTCAATTAGCCGGGCGTGGTGGCGGGCGCCTGT2160.2591854856128057No Hit
CGTAAGCTAGGGAAGCCCAGATTGGGAACCCAACGTAAGCATT2000.23998656075259783No Hit
CGTAAGCTTCCCAGGCAGGTTCCACCAGACAGGATGGGACGGG1640.19678897981713026No Hit
CGTAAGCTGATCAAGTTCTACAGTCCGTGTCGTAAGCTTTTTT1600.19198924860207828No Hit
CGTAACCTTAGAAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.18838945019078931No Hit
CGTAAACTCCACGGGTAAGCTTTTTTTTTTTTTTTTTTTTTTT1500.17998992056444837No Hit
CGTAAGCTCACACGCTTTTTTTTTTTTTTTTTTTTTTTTTTGG1500.17998992056444837No Hit
CGTAAGCTGCCGCGTTCCCGGCTGAGCTGAATAAGGGACCTCT1480.1775900549569224No Hit
CGTAAGCTCACACGCTTTTTTTTTTTTTTTTTTTTTTTTTGGG1480.1775900549569224No Hit
CGTAAGCTGGATTAAAACAACAACAACAACAAAAAGTTGTTCT1450.17399025654563346No Hit
GTTAAGCTTCGAGGTCCGACGATCTCGAAAAAGCAAAACAGTG1360.16319086131176655No Hit
CGTAAGCTGATCATTCTACAGTCCGTGTCGTAAGCTTTTTTTT1270.15239146607789963No Hit
CGTAAGCTGATCACAGTCCGTGTCGTAAGCTTTTTTTTTTTTT1240.14879166766661067No Hit
CGTAAGCTAGTGCGGGCAAACGCACTGATCCCGGGTGGAGAAG1200.14399193645155872No Hit
CGTAAGCTCACACGCTTTTTTTTTTTTTTTTTTTTTTTTGGGG1160.13919220523650677No Hit
CGCACGCTAGTGGGTCCGACGACCGTCCCAGCTAGTCTTTTTT1140.13679233962898077No Hit
CGTAAGCTTCGGTATCATGTCTTTTTTTCGACATGATTGGTAA1090.13079267561016583No Hit
CGTAAGCTTCGCGTCAGGGGCCAGGCCGTGGCAAGGACGCCTT1010.12119321318006192No Hit
CGTAACCTTAGAAGCTTTTTTTTTTTTTTTTTTTTTTTTTGGG950.11399361635748399No Hit
CGTAAGCTATACCCGCCTACCTCTCACCTCTCCCGGGTCACGG940.11279368355372099No Hit
CGTAACCTTAGAAGCTTTTTTTTTTTTTTTTTTTTTTTTTTGG930.11159375074995802No Hit
CGTAAGCTGCCGCCTTCCCGGCTGAGCTGAATAAGGGACCTCT920.11039381794619502No Hit
CGTAAGCTTGCACTGTAAGCTTTTTTTTTTTTTTTTTTTTTTT880.10559408673114305No Hit
CGTAAGCTTTGACGGTCATAGCTGGGGAGGGTGTGTCTTCCTG880.10559408673114305No Hit
CGTAAGCTGCCGCATTCCCGGCTGAGCTGAATAAGGGACCTCT880.10559408673114305No Hit
CGTAAGCTTGTAAGGCACAACGGGGACCATTTTATAAATCCTG850.10199428831985408No Hit
CCTAAGATGTCAAATTTAACTGGTTTCAGTATGAAAATATTTT840.1007943555160911No Hit
CGTAAGCTCACACGCTTTTTTTTTTTTTTTTTTTTTTTTTTTG840.1007943555160911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCAA501.72804E-1037.0360612
CAGTTCT403.7285645E-837.03605712
CGGCAAA600.037.03605713
CGTTCCC200.001823399337.03605713
CGGGCCA200.001823399337.03605713
CGCTTTT1300.037.03605713
TAGAAGC850.037.0138179
TGATCAC251.2211307E-437.0138138
TCACACG1300.037.0138138
CAGCATA403.7483915E-837.01381315
TGCGGGT650.037.01381310
ACGGGTA200.001828792937.01381311
CTCGGGG200.001828792937.0138139
AGCATAG452.5484042E-937.01381316
GAGCGGG200.001828792937.01381310
TCCACGG200.001828792937.0138138
TGTTAGA308.198651E-637.01381311
GTCAAAT200.001828792937.0138139
CGAGCGG200.001828792937.0138139
TCCCGCA200.001828792937.0138138
Produced by FastQC (version 0.11.5)