FastQCFastQC Report
Thu 26 May 2016
SRR2099261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57576
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTACCCACCCGATCCCGGCATCGGTACTTTTTTTTTTTT4670.811101847992219No Hit
ATCGGTACTGAAAGGGATGGGGGGAGGGGGGAGGGATAGCATT4220.7329442823398639No Hit
ATCGGTACAGCGAAACAAAAGCAGACTCTTTACAGAAACTGGA2900.503682089759622No Hit
ATCGGTGCAGCGCGGAAGGTCAAGATCAACCGCAAAAGCATCC2690.4672085591218563No Hit
ATCGGTACGCCACGGATCGGTACTTTTTTTTTTTTTTTTTTTT2590.4498402111991108No Hit
ACCGGTACGACCAGAACTCTGCGATCTTGCCGCTGGCCAGGTA2150.37341948033903016No Hit
ATCGGTGGGCAGGGGCCAGCCTCCTTAGCGACGACGCCGAACT1960.34041961928581355No Hit
ATTGGTACTAGCCAAGGAGGGTGAGGTGGGCTCCTCCGCGATG1330.23099902737251632No Hit
ATCGGTACCAACGGGGTTATCTGACCCACTGCTGCATGTGGGC1280.22231485341114354No Hit
ATCGGCACGCACCCAACGGCAACGTCTGGGACGCCACCCTGCG1260.2188411838265944No Hit
ATCGGTGCAGCGCGGGAAGGTCAAGATCAACCGCAAAAGCATC1240.21536751424204528No Hit
ATCGGTACTTTGCAGAATGCCAGACAGAAGACACTCCAAGTCA1140.1979991663192997No Hit
ATCGGTACCTCCCAGGAAAAGTGTTCACTTACACTTTTCCTTG1090.1893149923579269No Hit
ATCGGTACTGCACGACTCCTGCCAGTGTGAAGACAGACGGACT1040.18063081839655412No Hit
ATCGGTACGTCTGAGGAAATCACCATGCCCTCTACAAGAATGA920.15978880088925942No Hit
ATCGGTATGCAGCCCGCAGGCCGCCGGGCAGCCTGAGCGGAGG860.14936779213561208No Hit
ATCGGTACCCCATACAACATTCAAAAGGAGTCCACCCTTCATC830.1441572877587884No Hit
ATCGGTACGACTGTGAAAGATTGCGCTGGGCGTCTGAGGAGCA830.1441572877587884No Hit
ATAGGTGCCATGTGTCCCGACGATCCATGGAATCTTAGTCAGT830.1441572877587884No Hit
AACGATACCCGCACGAACGGTGGCTGCGATAGAAGTCGCGCCG770.13373627900514104No Hit
ATTGGGACAGCTAGTTGAGGGTTGCATGGCTGTATCCACACTG760.13199944421286647No Hit
ATCGGTACCTGGAGCCACACCGGAAACAAGTGCGGAGGCCGCC760.13199944421286647No Hit
ATCGGTACACCACCATGTATAATTGTTCATGGACAATTATACA740.12852577462831735No Hit
ATCGGTACTCTTTAGACACATGCACACATATGTTTATTACAGC730.12678893983604278No Hit
ATCGGTGCAGGCGTGGAGCCAGACCAATGACTCATTGCAATGA680.11810476587466999No Hit
ATCGGTACAATGTAGGGAACCCCCATTTCTGACCCATTAGTAG680.11810476587466999No Hit
ATCGGTACTGAAAGGGGATGGGGGGAGGGGGGAGGGATAGCAT650.11289426149784633No Hit
ATCGGTACCTACCGTCGCGTGCAGCCCATCAAGCTGGCTAGGG630.1094205919132972No Hit
TTCGGTACTCGATCAAAAACAGTGACTACGTTTTTGTATTCGA620.10768375712102264No Hit
ATCGGTACGCACAAAGAGAAGCACCAGGAGGGCGAGAAGGTGA610.10594692232874808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT2450.037.0000044
TATTTCG600.037.06
ATGGGGG355.508409E-737.017
GGAATCG355.508409E-737.04
CGGAACG200.001827447237.03
GGCAGGG308.175506E-637.08
TCGTTGC200.001827447237.02
GGTACGC2650.037.04
GGTACGA2250.037.04
GGTACCA1850.037.04
GTACCCC1450.037.05
GTACCAC452.5283953E-937.05
TACCCGG600.037.06
TACCCGC1650.037.06
TACCCAC452.5283953E-937.06
ACCGGAT200.001827447237.07
CTGTATT600.037.03
GTACATT200.001827447237.05
GTACATG452.5283953E-937.05
GTACATC600.037.05
Produced by FastQC (version 0.11.5)