FastQCFastQC Report
Thu 26 May 2016
SRR2099260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526607
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACAGTAAGGTAGTCAGTCCTTAGCAGCCCCATTTCCAATT22230.42213643191222294No Hit
TCGACAGTGTGTGGGTGATATGGATGACGGATGGCTGGCCGCT18030.3423805608356896No Hit
GCTACAGTGCGGTGCGCGGCGTCGCCTTCCTCGACGACGGTTC15960.30307231009082675No Hit
TCGACAGTATAGGTACAGTCCGACGATCCCAGCTCGACAGTTT13360.2536996279958299No Hit
TCGACAGCTCCTTGCTGGAAACCGGCGCCGACGACTATGTGGT11910.22616486298131247No Hit
GCTACAGTTCGCTGATGCATCCGCCGCAGCAAAGCGGTGTTTG11900.22597496805017783No Hit
TCGACAGTTCACAGGAAAATGAGAAACATCTCCTTGACGACTT11880.2255951781879086No Hit
GCTACAGTCTACTGAACGGGCTGGGTGCTGCGGTCCAATGGAC11860.2252153883256394No Hit
TCGACAGTAACGCGCTTGGTCTTGCCATTCGGCTTGCAATTCA11070.21021368876600577No Hit
ACGAAAGTCGTCAAATAATCTCCATCACTCGTTCACTGGAGAA8140.15457447394356702No Hit
TCGACAGTTCTAGGCAGTCGACAGTTTTTTTTTTTTTTTTTTT7850.14906752094066353No Hit
GCTACAGTGCAATAGGGAAGATGTCCTAAGCCCAGAGAACTGA7640.14507972738683686No Hit
GCTACAGTTCCATGCTCCACTCTCCCAGAGCCAGGCTTACTGT7610.14451004259343306No Hit
TCGACACCGTTGCATTGCCTCCAACAAAGCAGACATTGCGCTG7540.14318077807549082No Hit
GCTACAGTGCCGTGAGACAATGAGGTGGAATGGATTCAGGTTT7510.14261109328208701No Hit
GCTACAGTCCACTGCTTCCCCGAATGTGCCTTGGCCGATCTTG6360.12077317620160764No Hit
GCTACAGTTCCTTAGGGTCTATAATCCTGGCAGTTTATAACTC6350.12058328127047306No Hit
GCTACAGTAAGGTGCCGGAATGGCTCAGACGTGAGTCCCCGGA6330.12020349140820384No Hit
GCTACAGTTGTTTCATAAAACCAGCCAGTACACATTTCATTTT6220.1181146471657232No Hit
TCGACAGTACGGAGGCGACAGGAGCGAAACTCCATCTCAAATC6080.11545611812983876No Hit
TCGACAGTTGGGGGGAATCTGGAATAAAACAGTGAAAGAGCAT5980.11355716881849273No Hit
GCTACAGTAGAATGTTGAAATCTGAGTTTCTCTATGGGCCTTA5960.11317737895622353No Hit
GCTACAGTCGTGTAGGTGACCATGGAAGAACTTCAACTGTGAG5820.11051884992033909No Hit
GCTACAGTACGTTATGACAGCCACTCAGATTGTCATTTGAAAA5700.10824011074672385No Hit
GCTACAGTGCATTGGTTTCTCTGAATAGCCCTGGCTGTCCTGG5540.10520179184857019No Hit
TCGACAGTGTGTGGGGTGATATGGATGACGGATGGCTGGCCGC5430.10311294760608955No Hit
GCTACAGTTCAGTGCTACATGGCCCAGTTTCTCAGCAAAGTGG5390.10235336788155115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC251.2239846E-437.0447129
CGGTTAA200.001830142237.0447129
TCGATAT200.001830142237.0447129
GTCGTTA200.001830142237.0447129
CGCGTTT251.2239846E-437.0447129
CGGAGCG251.2239846E-437.0447129
TGCGTAC251.2239846E-437.0447129
TGCGCTA403.792411E-837.0376668
TTACGTA800.037.0376668
TATCGTG650.037.0376668
TTATTCG200.00183185337.0376668
TCGCGTG403.792411E-837.0376668
TACGTTA1300.037.0376668
TCCGGTA403.792411E-837.0376668
TAGCGTG551.2732926E-1137.0376638
TAACGCG1400.037.0376638
TTACGTC355.578913E-737.0376638
TGTCGTA1250.037.0376638
GTCGCGT1650.037.030627
GTAGCGT1350.037.030627
Produced by FastQC (version 0.11.5)