FastQCFastQC Report
Thu 26 May 2016
SRR2099259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134904
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACAGTAAGGTAGTCAGTCCTTAGCAGCCCCATTTCCAATT7120.5277827195635415No Hit
TCGACAGTGTGTGGGTGATATGGATGACGGATGGCTGGCCGCT5910.43808930795232165No Hit
GCTACAGTGCGGTGCGCGGCGTCGCCTTCCTCGACGACGGTTC5090.37730534305876773No Hit
TCGACAGTATAGGTACAGTCCGACGATCCCAGCTCGACAGTTT4000.2965071458222143No Hit
TCGACAGCTCCTTGCTGGAAACCGGCGCCGACGACTATGTGGT3730.27649291347921484No Hit
TCGACAGTTCACAGGAAAATGAGAAACATCTCCTTGACGACTT3670.2720453062918816No Hit
TCGACAGTAACGCGCTTGGTCTTGCCATTCGGCTTGCAATTCA3540.26240882405265964No Hit
GCTACAGTCTACTGAACGGGCTGGGTGCTGCGGTCCAATGGAC3520.2609262883235486No Hit
GCTACAGTTCGCTGATGCATCCGCCGCAGCAAAGCGGTGTTTG3500.2594437525944375No Hit
TCGACACCGTTGCATTGCCTCCAACAAAGCAGACATTGCGCTG2780.20607246634643897No Hit
TCGACAGTTCTAGGCAGTCGACAGTTTTTTTTTTTTTTTTTTT2650.196435984107217No Hit
ACGAAAGTCGTCAAATAATCTCCATCACTCGTTCACTGGAGAA2590.19198837691988377No Hit
GCTACAGTGCCGTGAGACAATGAGGTGGAATGGATTCAGGTTT2550.18902330546166163No Hit
GCTACAGTTCCATGCTCCACTCTCCCAGAGCCAGGCTTACTGT2520.18679950186799502No Hit
GCTACAGTGCAATAGGGAAGATGTCCTAAGCCCAGAGAACTGA2380.1764217517642175No Hit
GCTACAGTCCACTGCTTCCCCGAATGTGCCTTGGCCGATCTTG2240.16604400166044No Hit
GCTACAGTTGTTTCATAAAACCAGCCAGTACACATTTCATTTT1920.14232342999466288No Hit
GCTACAGTAAGGTGCCGGAATGGCTCAGACGTGAGTCCCCGGA1870.13861709067188518No Hit
GCTACAGTAGAATGTTGAAATCTGAGTTTCTCTATGGGCCTTA1840.1363932870782186No Hit
TCGACAGTGTGTGGGGTGATATGGATGACGGATGGCTGGCCGC1810.134169483484552No Hit
GCTACAGTCGTGTAGGTGACCATGGAAGAACTTCAACTGTGAG1800.13342821561999643No Hit
GCTACAGTTCCTTAGGGTCTATAATCCTGGCAGTTTATAACTC1770.13120441202632982No Hit
TCGACAGTTGGGGGGAATCTGGAATAAAACAGTGAAAGAGCAT1770.13120441202632982No Hit
GCTACAGTACGTTATGACAGCCACTCAGATTGTCATTTGAAAA1610.11934412619344127No Hit
GCTACAGTTCAGTGCTACATGGCCCAGTTTCTCAGCAAAGTGG1530.11341398327699696No Hit
GCTACAGTTCGTTAGTACACTTGGCAGAGGGAAGATGCCCTTT1510.11193144754788592No Hit
TCGACAGCTCCTTGCTTGAAACCGGCGCCGACGACTATGTGGT1490.11044891181877484No Hit
TCGACAGTACGGAGGCGACAGGAGCGAAACTCCATCTCAAATC1480.1097076439542193No Hit
GCTACAGTCTTTTCAAAGGAACCTTTAAAGTTTCTAGGTGTGT1350.10007116171499733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT308.225907E-637.02077515
AGGCGAC308.225907E-637.02077513
AGGTAGA200.00183112637.0207713
TCGGCCG251.2238046E-437.0207713
TCGTCCG501.7644197E-1037.0207713
CTGGATT200.00183112637.0207715
GGTACAG501.7644197E-1037.00704612
ATAGGTA501.7644197E-1037.0070469
CGCGCTT251.2264807E-437.00704611
GCTGATG251.2264807E-437.00704611
CTGAACG452.579327E-937.00704612
AACGCGC251.2264807E-437.0070469
TTTGGGG251.2264807E-437.00704611
ATGCGAT251.2264807E-437.0070469
GCCGTGA308.24688E-637.0070469
ACGCGCT251.2264807E-437.00704610
AGACTAG200.001834465937.0070429
TCGTTAG355.573984E-737.0070429
ACGTTCA355.573984E-737.0070429
GCGGTAG200.001834465937.0070429
Produced by FastQC (version 0.11.5)