FastQCFastQC Report
Thu 26 May 2016
SRR2099258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279812
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACCAGATGTCTCGTGTGCTGGTGCGCCAGCGCCACGGTGA55621.9877632124426399No Hit
CATGTCAGTCACAGCATTCTCTCCTTTTTATTCTCCCAGTGGG30231.0803682472517262No Hit
CATGTCAGATATTATCAGATCCTAGGTCTGCTAGGAAGAATCT27180.9713664889282804No Hit
CATGTCAGGCAGTGCAGAGTCTCTCTCAGGGCGGCAGAGGCTG12580.4495875802324418No Hit
CATGTCAGATATTGATCAGATCCTAGGTCTGCTAGGAAGAATC8630.30842136863322517No Hit
CATGTCAGTTTTAGCCTGGCACCTGGTGCATAGAGATGGTCCT8550.3055623061198233No Hit
CATGTCAGGGGAGGTGCGGGCTCCACGATATCCCCAAGCTGCG7100.25374179806441466No Hit
CATGGAAGTCGAGGATGCAGCTCTTCAGATGTCCGCTGGCTGT6710.23980386831158065No Hit
CATGTCAGCCCCACAAACTCCTGGCTTCAAGAAATTCATCCAT6230.2226494932311695No Hit
CATGTCAGTATACTGAGCCCTGGACCCTCCCTTCCCTGGATCA5760.2058525009649336No Hit
CATGTCAGTATTTTGGCTCAGCAGTTAAGAGCACCGACTGTTC5250.18762597744199677No Hit
CATGCCAGTTTTTGGATTAGAGGCGTGAGCCACCGCACCTGGC5130.183337383671894No Hit
CATGTCAGTGTTAGGCAGGGGAGTGGGGGTGGGTGGATATGGG4620.16511086014895715No Hit
CATGTCAGATATTTATCAGATCCTAGGTCTGCTAGGAAGAATC4550.16260918044973052No Hit
CATGTCATGCATCATCCAAGCCATGTCAGTTTTTTTTTTTTTT4200.1501007819535974No Hit
CATGTCAGATATTCATCAGATCCTAGGTCTGCTAGGAAGAATC4080.14581218818349465No Hit
CATGTCGATTGCCGCGGCATGCGGCCCACGGCGCTGGGCGCGC3650.13044472717395966No Hit
CATGTCAGTTAGTGGCAAAGGTGGGTGGATTTCTGAGTTCAAG3610.12901519591725874No Hit
CATGTCAGATCTAGAACAGCCCACCATGAGAAGTGGAGGGTCT3580.12794304747473303No Hit
CATCTCTGAATGTGGTTAAGCAAAGTGTGGTATATTCCTCAAG3290.11757894586365131No Hit
CATGTCAGTGTTAGGGCAGGGGAGTGGGGGTGGGTGGATATGG3280.11722156304947606No Hit
CATGTCAGTCGTACGTATCAACCCCTCAAGGGTTGGTGAAGAG3210.11471988335024945No Hit
CATGTCATGGAAGGTGTAGGCCTCCACCAGCAGCATCCAGACG3110.11114605520849713No Hit
CACGTCAGTCTTCGGTGATTGAATCCGCTTCAGACATACCCAG2870.10256886766829157No Hit
CATGTCAGTCCCATATCAACCCCTCAAGGGTTGGTGAAGAGAT2870.10256886766829157No Hit
CATACCAGATGTCTCGTGTGCTGGTGCGCCAGATGGTGTCGGT2850.10185410203994111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC200.001806953937.13493737
CCAGACG355.4531847E-737.13493737
TAATATG200.001834191437.02200713
CGGAAAC200.001834191437.02200713
CGGAGGG200.001834191437.02200713
TTACGAC251.2269366E-437.02200313
AGCCGTC700.037.0021487
GCCCATA355.6084536E-737.0021488
ACGTTCC200.001839031637.0021489
GAGTCAA403.8136932E-837.0021488
CGGTTGG200.001839031637.0021489
TAGCGCT403.8136932E-837.0021488
TTCACGG200.001839031637.0021489
GCGCCGT200.001839031637.0021488
GGTCATC600.037.0021488
TATGCTA200.001839031637.00214812
AGAGTTG403.8136932E-837.0021487
TACATCG200.001839031637.00214810
CGTAGCA308.286199E-637.00214811
AGCATAA200.001839031637.0021487
Produced by FastQC (version 0.11.5)