FastQCFastQC Report
Thu 26 May 2016
SRR2099257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69690
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACCAGATGTCTCGTGTGCTGGTGCGCCAGCGCCACGGTGA18532.6589180657196154No Hit
CATGTCAGTCACAGCATTCTCTCCTTTTTATTCTCCCAGTGGG9531.3674845745444109No Hit
CATGTCAGATATTATCAGATCCTAGGTCTGCTAGGAAGAATCT8641.2397761515281962No Hit
CATGTCAGGCAGTGCAGAGTCTCTCTCAGGGCGGCAGAGGCTG4330.6213230018654039No Hit
CATGTCAGTTTTAGCCTGGCACCTGGTGCATAGAGATGGTCCT3140.4505667958100158No Hit
CATGTCAGATATTGATCAGATCCTAGGTCTGCTAGGAAGAATC2870.41182379107475964No Hit
CATGTCAGGGGAGGTGCGGGCTCCACGATATCCCCAAGCTGCG2230.31998852059118954No Hit
CATGGAAGTCGAGGATGCAGCTCTTCAGATGTCCGCTGGCTGT2150.3085091117807433No Hit
CATGCCAGTTTTTGGATTAGAGGCGTGAGCCACCGCACCTGGC1970.2826804419572392No Hit
CATGTCAGTATACTGAGCCCTGGACCCTCCCTTCCCTGGATCA1900.27263595924809875No Hit
CATGTCAGCCCCACAAACTCCTGGCTTCAAGAAATTCATCCAT1860.2668962548428756No Hit
CATGTCAGATATTTATCAGATCCTAGGTCTGCTAGGAAGAATC1520.21810876739847898No Hit
CATGTCAGTTGAACGTTCGTGCCCAATGATGGCTGCCTGAACT1480.21236906299325586No Hit
CATGTCAGTGTTAGGCAGGGGAGTGGGGGTGGGTGGATATGGG1460.2094992107906443No Hit
CATGTCGATTGCCGCGGCATGCGGCCCACGGCGCTGGGCGCGC1350.19371502367628068No Hit
CATGTCAGATATTCATCAGATCCTAGGTCTGCTAGGAAGAATC1340.19228009757497488No Hit
CATGTCAGATCTAGAACAGCCCACCATGAGAAGTGGAGGGTCT1280.1836705409671402No Hit
CATGTCATGCATCATCCAAGCCATGTCAGTTTTTTTTTTTTTT1190.17075620605538816No Hit
CATGTCATGGAAGGTGTAGGCCTCCACCAGCAGCATCCAGACG1140.16358157554885924No Hit
CATGTCAGTGTTAGGGCAGGGGAGTGGGGGTGGGTGGATATGG1020.14636246233318984No Hit
CATGTCAGTTAGTGGCAAAGGTGGGTGGATTTCTGAGTTCAAG1000.1434926101305783No Hit
CATGTCAGTCGTACGTATCAACCCCTCAAGGGTTGGTGAAGAG950.13631797962404937No Hit
GATCTCAGGGCATTTTTTTTCCCCTCCTCTCAATTTTATCTCT930.1334481274214378No Hit
CATACCAGATGTCTCGTGTGCTGGTGCGCCAGATGGTGTCGGT930.1334481274214378No Hit
CACGTCAGTCTTCGGTGATTGAATCCGCTTCAGACATACCCAG920.132013201320132No Hit
CATGTCAGCGAATCAGTCCGACGATCCCCTATAGTGAGTCGTA880.12627349691490888No Hit
CATGTCAGTTGGTGGTGAGGACTAGTGCCTGAAGATGTCCTCT840.12053379250968575No Hit
CATCTCTGAATGTGGTTAAGCAAAGTGTGGTATATTCCTCAAG840.12053379250968575No Hit
CACGTCATGTACACAGATCATCATGAGCGCCACTCTCGGTGAC800.11479408810446262No Hit
CATGTCAGTTTGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.11479408810446262No Hit
CATGTCAGCTTCTGTTTGCTTGGCCTGACCTTGACGATTCTAG770.11048930980054528No Hit
CATGTCAGTTTTCTCGCTCAAAGTCTCGATCTTCACATTCACG750.1076194575979337No Hit
CATGTCAGACGCTAGGACTTCTACAATCAGGCGTCTAGACCAG730.10474960539532215No Hit
CATGTCAGGTCTCGCGGGCTCAACCCTTCTGCAAGCCAAGATT700.10044482709140479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTCC900.037.04313318
TTAGAGG200.001819633237.04313317
GGATTAG200.001819633237.04313314
AGAGGCG200.001819633237.04313319
ACGTATC355.511338E-737.01652513
CTCGTGT1950.037.0165213
ACGTTCG200.001826074937.0165213
ACCTCAT200.001826074937.0165220
TGGATTA200.001826074937.0165213
GAGGCGT200.001826074937.0165220
TGTCATA308.215631E-636.9899523
GTCATAT308.215631E-636.9899524
TGTCACG355.542497E-736.9899523
TTAGGGC308.215631E-636.98995211
TTAGGCA308.215631E-636.98995211
CAGCGGA308.215631E-636.9899526
GTCGATT650.036.9899524
GAGCTCT308.215631E-636.9899528
GAGCTCG355.542497E-736.9899528
GAGCTCA308.215631E-636.9899528
Produced by FastQC (version 0.11.5)