FastQCFastQC Report
Thu 26 May 2016
SRR2099255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78185
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAGTCCCCGCGCTAGTCTTTTTTTTTTTTTTTTTTTTTTT7941.0155400652299034No Hit
AGCTAGTCACCCAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.8262454434993926No Hit
AGCTAGTCCTATACAGTCCGACGATCCGCTGAGCTAGTCTTTT6240.798107053782695No Hit
AGCTAGACACCCAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.587069130907463No Hit
GGCCAGTCACCAGCATCCACGACGCCGCGGTGGCGGGCTACCT4580.5857901131930677No Hit
AGCTAGTCAGTTGTCTACAGTCCGACGATCTCCACAGCTAGTC2960.3785892434610219No Hit
AGCTAATAAGCTGAGTCGACGCAGTTCCACATCAGACAACCAG2860.3657990663170685No Hit
AGCTAGCCCCCGAGGTCACTTGGGAAACTCACTTGACCTCTTA2010.2570825605934642No Hit
AGCTAGTCTTAATTTCTCCAGTCCGACGATCCGGATAGCTAGT1830.23406024173434803No Hit
AGCTAGTCACCCACAAAAGATGGCCTAGTCGGCCATCACTGGA1730.22127006459039458No Hit
AGCTAGTCAGGCAGGAACAAAGAAGACCTCCCACTAGGAAGAA1560.19952676344567374No Hit
AGCTAGTCCCGCGCCGACGATCATTCTAGCTAGTCTTTTTTTT1520.19441069258809235No Hit
AGCTAGTCGCCGGATTTTACATTCAATTGATGATGCAAGTGGA1420.1816205154441389No Hit
AGCTAGTCCCTGTGGGGAACCTGAGTCATGTCATGTAATGTGA1360.17394640915776685No Hit
AGCAAGTAGTGTGGGCCAGGGGAGTGCTAGCCGTGAGCCGGGA1320.16883033830018546No Hit
AGCCTGTCTGGGCGGGATGCCCACGCGGGTCAGAAGCCGCAGC1290.16499328515699943No Hit
AACCAGTCCAGGCGGTTAGCAAAAATCTCTGACATTTCATCCA1290.16499328515699943No Hit
AGTTAATCGTCATGTGTTCCGTGCCGATGCACGCCAAAAAGAG1200.15348212572744133No Hit
AGCTAGTCCGACAGGGAAGGGAGCCACAGAGTTAACGTTTTTC1170.1496450725842553No Hit
AGCTAGTCCCCGCGCTAGTCTTTTTTTTTTTTTTTTTTTTTTC1140.14580801944106928No Hit
AGCTGGTACAAGATTGATTTGCATGGTTCAACATGCAAATCAA1130.1445290017266739No Hit
AGCTAGTCTAGTGCGATCGTTCTAGCTAGTCTTTTTTTTTTTT1010.12918078915392978No Hit
AGCTAGTCCCGGTGCAGGGCAGCCGCATCCGGGCTCTGCTCGG990.1266227537251391No Hit
AGCTCGGCCTACGGCCAGTACCAGCACGACCTCGACGCGACGC960.12278570058195305No Hit
AGCTAGACCCCGCGCTAGTCTTTTTTTTTTTTTTTTTTTTTTT950.12150668286755771No Hit
AGCTAGTCTGTCTGGTGTCGGGGTGGACGCTGCTTCGGTGCAA940.12022766515316237No Hit
AGCTGGTACAAGATTTGCATGGTTCAACATGCAAATCAATCAA920.11766962972437169No Hit
AGCTAGTCACCCAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTG900.11511159429558099No Hit
AGCTGGTACAAGATGATTTGCATGGTTCAACATGCAAATCAAT890.11383257658118566No Hit
AGCAAGTGAGCTAAGGACACTGGGGTTGGTAAGTGGCAGCAGA870.11127454115239495No Hit
TGCTAGTTACAACGCTCGCACGAGCTAGTCTTTTTTTTTTTTT870.11127454115239495No Hit
AGCTAGTCACCCAGTCTTTTTTTTTTTTTTTTTTTTTTTTGCT840.10743748800920892No Hit
AGCTAGTCTAGTGGCGATCGTTCTAGCTAGTCTTTTTTTTTTT830.10615847029481358No Hit
AGCTAGTCCGCAAGAGTTCTACAGTCCGACGATCTGTCCAGCT820.10487945258041824Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCTAGGCAGCTTCCTTCCCAGCCACTGGCTGCCAGAAACTCA810.1036004348660229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTCC200.001826792337.01920714
CAACGCT200.001826792337.01920710
GCGAAGA200.001826792337.01920711
ATGTGTT200.001826792337.01920712
CTATACC251.2193908E-437.01920714
CCGGATT355.5197415E-737.01920710
GGAAGGG200.001826792337.01920715
CCCCGAC200.001826792337.01920712
TGTCTAC452.5411282E-937.01920712
GGGCGGT200.001826792337.01920710
TGTTCCG200.001826792337.01920715
CCGAGGT200.001826792337.01920710
GTTCCGT200.001826792337.01920716
AAGATTT200.001826792337.01920710
CTCCTAT251.2193908E-437.01920711
TATACCT251.2193908E-437.01920715
TATACAG551.2732926E-1137.01920710
TGTGTTC200.001826792337.01920713
CGAAGAA200.001826792337.01920712
CGCGCTA1300.037.01920311
Produced by FastQC (version 0.11.5)